%0 Journal Article %A Zuber, Chantal %A Mitteregger, Gerda %A Schuhmann, Natascha %A Rey, Clémence %A Knackmuss, Stefan %A Rupprecht, Wolfgang %A Reusch, Uwe %A Pace, Claudia %A Little, Melvyn %A Kretzschmar, Hans A. %A Hallek, Michael %A Büning, Hildegard %A Weiss, Stefan %T Delivery of single-chain antibodies (scFvs) directed against the 37/67 kDa laminin receptor into mice via recombinant adeno-associated viral vectors for prion disease gene therapy %D 2008 %J Journal of General Virology, %V 89 %N 8 %P 2055-2061 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.83670-0 %I Microbiology Society, %X The 37/67 kDa laminin receptor (LRP/LR) acts as a receptor for prions providing a promising target for the treatment of prion diseases. Recently, we selected anti-LRP/LR single-chain antibodies (scFvs) and proved a reduction of the peripheral PrPSc propagation by passive immunotransfer into scrapie-infected mice. Here, we report the development of an in vivo gene delivery system based on adeno-associated virus (AAV) vectors expressing scFvs-S18 and -N3 directed against LRP/LR. Transduction of neuronal and non-neuronal cells with recombinant (r)AAV serotype 2 vectors encoding scFv-S18, -N3 and -C9 verified the efficient secretion of the antibodies. These vectors were administered via stereotactic intracerebral microinjection into the hippocampus of C57BL/6 mice, followed by intracerebral inoculation with 10 % RML at the same site 2 weeks post-injection of rAAV. After 90 days post-infection, scFv-S18 and -N3 expression resulted in the reduction of peripheral PrPSc propagation by approximately 60 and 32 %, respectively, without a significant prolongation of incubation times and survival. Proof of rAAV vector DNA in spleen samples by real-time PCR strongly suggests a transport or trafficking of rAAV from the brain to the spleen, resulting in rAAV-mediated expression of scFv followed by reduced PrPSc levels in the spleen most likely due to the blockage of the prion receptor LRP/LR by scFv. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83670-0