1887

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Volume 89, Issue 8

Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (G) protein in human serum Free

Abstract

Human metapneumovirus (hMPV) strains are classified into two genetic groups, A and B, each of which is further divided in two genetic subgroups, A1, A2, B1 and B2. hMPV encodes two major surface glycoproteins, the fusion (F) and attachment (G) proteins, which may be immunogenic and protective antigens. Although the amino acid sequences of hMPV F protein are highly conserved, those of the G protein are highly variable with low amino acid identity between the two groups. To address the antigenic variation between the genetic subgroups, we developed an immunofluorescence assay (IFA) method using (Tn5) insect cells infected with each recombinant baculovirus-expressed hMPV G (Bac-G) protein of the four genetic subgroups. The titre of each antibody to the four Bac-G proteins was measured by the IFA in 12 paired serum samples obtained from children infected with hMPV of each genetic subgroup. Although 11 of the 12 acute-phase serum samples in paired samples were negative for the antibody to any Bac-G proteins, all of the convalescent-phase serum samples in those paired samples were positive for the antibody to only one of the four Bac-G proteins of the infecting genotype of hMPV. Since the antibody response to hMPV G protein was transient and genetic subgroup-specific without cross-reactivity, four genetic subgroups on the basis of hMPV G protein could be identified as different serotypes. This assay may be useful for the study of immune responses of humans to different hMPV strains, especially for clarifying the risk of reinfection with hMPV.

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2008-08-01
2024-04-19
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References

  1. Agapov E., Sumino K. C., Gaudreault-Keener M., Storch G. A., Holtzman M. J. 2006; Genetic variability of human metapneumovirus infection: evidence of a shift in viral genotype without a change in illness. J Infect Dis 193:396–403 [CrossRef]
     [Google Scholar]
  2. Anderson L. J., Bingham P., Hierholzer J. C. 1988; Neutralization of respiratory syncytial virus by individual and mixtures of F and G protein monoclonal antibodies. J Virol 62:4232–4238
     [Google Scholar]
  3. Bastien N., Liu L., Ward D., Taylor T., Li Y. 2004; Genetic variability of the G glycoprotein gene of human metapneumovirus. J Clin Microbiol 42:3532–3537 [CrossRef]
     [Google Scholar]
  4. Biacchesi S., Skiadopoulos M. H., Boivin G., Hanson C. T., Murphy B. R., Collins P. L., Buchholz U. J. 2003; Genetic diversity between human metapneumovirus subgroups. Virology 315:1–9 [CrossRef]
     [Google Scholar]
  5. Biacchesi S., Skiadopoulos M. H., Yang L., Lamirande E. W., Tran K. C., Murphy B. R., Collins P. L., Buchholz U. J. 2004; Recombinant human metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate. J Virol 78:12877–12887 [CrossRef]
     [Google Scholar]
  6. Biacchesi S., Pham Q. N., Skiadopoulos M. H., Murphy B. R., Collins P. L., Buchholz U. J. 2005; Infection of nonhuman primates with recombinant human metapneumovirus lacking the SH, G, or M2–2 protein categorizes each as a nonessential accessory protein and identifies vaccine candidates. J Virol 79:12608–12613 [CrossRef]
     [Google Scholar]
  7. Ebihara T., Endo R., Kikuta H., Ishiguro N., Ishiko H., Hara M., Takahashi Y., Kobayashi K. 2004a; Human metapneumovirus infection in Japanese children. J Clin Microbiol 42:126–132 [CrossRef]
     [Google Scholar]
  8. Ebihara T., Endo R., Ishiguro N., Nakayama T., Sawada H., Kikuta H. 2004b; Early reinfection with human metapneumovirus in an infant. J Clin Microbiol 42:5944–5946 [CrossRef]
     [Google Scholar]
  9. Gerna G., Sarasini A., Percivalle E., Genini E., Campanini G., Grazia Revello M. 2005; Simultaneous detection and typing of human metapneumovirus strains in nasopharyngeal secretions and cell cultures by monoclonal antibodies. J Clin Virol 35:113–116
     [Google Scholar]
  10. Hancock G. E., Smith J. D., Heers K. M. 2000; Serum neutralizing antibody titers of seropositive chimpanzees immunized with vaccines coformulated with natural fusion and attachment proteins of respiratory syncytial virus. J Infect Dis 181:1768–1771 [CrossRef]
     [Google Scholar]
  11. Ishiguro N., Ebihara T., Endo R., Ma X., Kikuta H., Ishiko H., Kobayashi K. 2004; High genetic diversity of the attachment (G) protein of human metapneumovirus. J Clin Microbiol 42:3406–3414 [CrossRef]
     [Google Scholar]
  12. Ishiguro N., Ebihara T., Endo R., Ma X., Shirotsuki R., Ochiai S., Ishiko H., Kikuta H. 2005; Immunofluorescence assay for detection of human metapneumovirus-specific antibodies by use of baculovirus-expressed fusion protein. Clin Diagn Lab Immunol 12:202–205
     [Google Scholar]
  13. Leung J., Esper F., Weibel C., Kahn J. S. 2005; Seroepidemiology of human metapneumovirus (hMPV) on the basis of a novel enzyme-linked immunosorbent assay utilizing hMPV fusion protein expressed in recombinant vesicular stomatitis virus. J Clin Microbiol 43:1213–1219 [CrossRef]
     [Google Scholar]
  14. Ludewick H. P., Abed Y., van Niekerk N., Boivin G., Klugman K. P., Madhi S. A. 2005; Human metapneumovirus genetic variability, South Africa. Emerg Infect Dis 11:1074–1078 [CrossRef]
     [Google Scholar]
  15. Ma X., Endo R., Ebihara T., Ishiguro N., Ishiko H., Kikuta H. 2005; Production and characterization of neutralizing monoclonal antibodies against human metapneumovirus F protein. Hybridoma (Larchmt) 24:201–205 [CrossRef]
     [Google Scholar]
  16. Mackay I. M., Bialasiewicz S., Waliuzzaman Z., Chidlow G. R., Fegredo D. C., Laingam S., Adamson P., Harnett G. B., Rawlinson W. other authors 2004; Use of the P gene to genotype human metapneumovirus identifies 4 viral subtypes. J Infect Dis 190:1913–1918 [CrossRef]
     [Google Scholar]
  17. Mackay I. M., Bialasiewicz S., Jacob K. C., McQueen E., Arden K. E., Nissen M. D., Sloots T. P. 2006; Genetic diversity of human metapneumovirus over 4 consecutive years in Australia. J Infect Dis 193:1630–1633 [CrossRef]
     [Google Scholar]
  18. Maertzdorf J., Wang C. K., Brown J. B., Quinto J. D., Chu M., de Graaf M., van den Hoogen B. G., Spaete R., Osterhaus A. D., Fouchier R. A. 2004; Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J Clin Microbiol 42:981–986 [CrossRef]
     [Google Scholar]
  19. Peret T. C., Abed Y., Anderson L. J., Erdman D. D., Boivin G. 2004; Sequence polymorphism of the predicted human metapneumovirus G glycoprotein. J Gen Virol 85:679–686 [CrossRef]
     [Google Scholar]
  20. Skiadopoulos M. H., Biacchesi S., Buchholz U. J., Riggs J. M., Surman S. R., Amaro-Carambot E., McAuliffe J. M., Elkins W. R., St Claire M. other authors 2004; The two major human metapneumovirus genetic lineages are highly related antigenically, and the fusion (F) protein is a major contributor to this antigenic relatedness. J Virol 78:6927–6937 [CrossRef]
     [Google Scholar]
  21. Skiadopoulos M. H., Biacchesi S., Buchholz U. J., Amaro-Carambot E., Surman S. R., Collins P. L., Murphy B. R. 2006; Individual contributions of the human metapneumovirus F, G, and SH surface glycoproteins to the induction of neutralizing antibodies and protective immunity. Virology 345:492–501 [CrossRef]
     [Google Scholar]
  22. van den Hoogen B. G., de Jong J. C., Groen J., Kuiken T., de Groot R., Fouchier R. A., Osterhaus A. D. 2001; A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7:719–724 [CrossRef]
     [Google Scholar]
  23. van den Hoogen B. G., Herfst S., Sprong L., Cane P. A., Forleo-Neto E., de Swart R. L., Osterhaus A. D., Fouchier R. A. 2004; Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis 10:658–666 [CrossRef]
     [Google Scholar]
  24. van den Hoogen B. G., Herfst S., de Graaf M., Sprong L., van Lavieren R., van Amerongen G., Yuksel S., Fouchier R. A., Osterhaus A. D., de Swart R. L. 2007; Experimental infection of macaques with human metapneumovirus induces transient protective immunity. J Gen Virol 88:1251–1259 [CrossRef]
     [Google Scholar]
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Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (G) protein in human serum
J. Gen. Virol. 89, 1970 (2008); https://doi.org/10.1099/vir.0.83679-0
/content/journal/jgv/10.1099/vir.0.83679-0
/content/journal/jgv/10.1099/vir.0.83679-0
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