1887

Abstract

Summary

Inoculation of inactivated 146S foot-and-mouth disease virus particles into guineapigs elicited the formation of neutralizing antibody and the serum had a 10-fold higher titre in radioimmunoassay (RIA) with 146S particles than with the 12S virus subunit. In contrast, a single inoculation of the 12S subunit or the isolated polypeptide VP1 elicited the formation of antibody having a much lower titre in RIA with the 146S particle than with the 12S subunit and low or undetectable neutralizing activity. However, sera from guinea-pigs given two or more inoculations of the 12S subunit or VP1 had neutralizing activity. The level in the anti-VP1 serum was lower than that in the anti-12S serum and both were much lower than that in animals receiving two inoculations of the 146S particle. The neutralizing activity elicited by the three antigens was absorbed by the homologous antigen. In contrast, neither the 12S subunit nor VP1 absorbed the anti-146S neutralizing antibody and VP1 did not absorb the anti-12S subunit neutralizing antibody. However, the 12S subunit partly absorbed the neutralizing activity elicited by VP1. The results are compatible with a model in which the 146S particle elicits a spectrum of neutralizing antibodies which are completely absorbed by the homologous particle but only partially by the 12S subunit or VP1. The results are discussed in relation to the structural features required for the production of neutralizing antibody.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-2-375
1982-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/2/JV0630020375.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-2-375&mimeType=html&fmt=ahah

References

  1. Bachrach H. L., Moore D. M., Mckercher P. D., Polatnick J. 1975; Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. Journal of Immunology 115:1636–1641
    [Google Scholar]
  2. Boothroyd J. C., Harris T. J. R., Rowlands D. J., Lowe P. A. 1982; The nucleotide sequence of cDNA coding for the structural protein of FMDV. Gene 17:153–161
    [Google Scholar]
  3. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature, London 199:1168–1170
    [Google Scholar]
  4. Cartwright B., Brown F., Chapman W. G. 1980; Serological and immunological relationships between the 146S and 12S particles of foot-and-mouth disease virus. Journal of General Virology 50:369–375
    [Google Scholar]
  5. Cartwright B., Chapman W. G., Sharpe R. T. 1982; The stimulation by heterotypic antigens of foot-and- mouth disease virus antibodies in vaccinated cattle. Research in Veterinary Science 32:338–342
    [Google Scholar]
  6. Dunker A. K. 1979; The structure of picomaviruses: classification of the bonding networks. Virology 97:141–150
    [Google Scholar]
  7. Hordern J. S., Leonard J. D., Scraba D. G. 1979; Structure of the Mengo virion. VI. Spatial relationships of the capsid polypeptides as determined by chemical cross-linking analysis. Virology 97:131–140
    [Google Scholar]
  8. Icenogle J., Gilbert S. F., Grieves J., Anderegg J., Rueckert R. 1981; A neutralizing monoclonal antibody against poliovirus: its reaction with related antigens. Virology 115:211–215
    [Google Scholar]
  9. Kaaden O. R., Adam K. H., Strohmaier K. 1977; Induction of neutralizing antibodies and immunity in vaccinated guinea pigs by cyanogen bromide peptides of VP3 of foot-and-mouth disease virus. Journal of General Virology 34:397–400
    [Google Scholar]
  10. Kleid D. G., Yansura D., Small B., Dowbenko D., Moore D. M., Grubman M. J., McKercher P. D., Morgan D. O., Robertson B. H., Bachrach H. L. 1981; Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine. Science 214:1125–1129
    [Google Scholar]
  11. Laporte J., Grosclaude J., Wantyghem J., Bernard S., Rouze P. 1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fievre aphteuse par des serums provenant de pores immunises a l'aide d’une proteine virale purifiee. Comptes rendus hebdomadaires des seances de I'Academie des sciences 276D:3399–3401
    [Google Scholar]
  12. Meloen R. H., Briaire J. 1980; A study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12S subunits with antisera against the structural proteins. Journal of General Virology 51:107–116
    [Google Scholar]
  13. Meloen R. H., Rowlands D. J., Brown F. 1979; Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polio viruses. Journal of General Virology 45:761–763
    [Google Scholar]
  14. Vasquez C., Denoya C. D., La Torre J. L., Palma E. L. 1979; Structure of foot-and-mouth disease virus capsid. Virology 97:195–200
    [Google Scholar]
  15. Wild T. F., Burroughs J. N., Brown F. 1967; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-2-375
Loading
/content/journal/jgv/10.1099/0022-1317-63-2-375
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error