1887

Abstract

SUMMARY

The fusion protein of respiratory syncytial virus was purified by affinity chromatography using a monoclonal antibody. Under various conditions the protein was recovered as a 145K dimer or a 70K monomer. The 70K monomer was composed of disulphide-linked fragments of 48K and 23K. Polyclonal rabbit serum produced to the dimerized fusion protein neutralized virus but did not inhibit fusion, while rabbit serum to the 2-mercaptoethanol-treated dimerized protein neutralized virus and inhibited fusion of infected cells. Only the latter serum strongly recognized the 23K fragment when studied by Western blot analysis.

Keyword(s): characterization , fusion protein and RSV
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/content/journal/jgv/10.1099/0022-1317-66-3-409
1985-03-01
2024-04-18
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References

  1. Belshe R. B., Van Voris L. P., Mufson M. A. 1982; Parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. Journal of Infectious Diseases 145:311–319
    [Google Scholar]
  2. Cash P., Wunner W. H., Pringle C. R. 1977; A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virus. Virology 82:369–379
    [Google Scholar]
  3. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Reviews of Infectious Diseases 2:40–61
    [Google Scholar]
  4. Dubovi E. J. 1982; Analysis of proteins synthesized in respiratory syncytial virus-infected cells. Journal of Virology 42:372–378
    [Google Scholar]
  5. Fernie B. F., Gerin J. L. 1982; Immunochemical identification of viral and non-viral proteins of respiratory syncytial virus virion. Infection and Immunity 37:243–249
    [Google Scholar]
  6. Fernie B. F., Cote P. J. Jr, Gerin J. L. 1982; Classification of hybridomas to respiratory syncytial virus glycoproteins. Proceedings of the Society for Experimental Biology and Medicine 171:266–271
    [Google Scholar]
  7. Gething M. J., White J. M., Waterfield M. D. 1978; Purification of the fusion protein of Sendai virus: analysis of the NH2-terminal sequence generated during precursor activation. Proceedings of the National Academy of Sciences, U.S.A. 75:2737–2740
    [Google Scholar]
  8. Gruber C., Levine S. 1983; Respiratory syncytial virus polypeptides. III. The envelope-associated proteins. Journal of General Virology 64:825–832
    [Google Scholar]
  9. Hsu M., Scheid A., Choppin P. W. 1981; Activation of the Sendai virus fusion protein (F) involves conformational change with exposure of a new hydrophobic region. Journal of Biological Chemistry 256:3557–3563
    [Google Scholar]
  10. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1969; Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
    [Google Scholar]
  11. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyle G., Reid J. L., Chanock R. M., Parrott R. H. 1973; Epidemiology of respiratory syncytial infection in Washington, D.C.I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  12. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  13. Merz D. C., Scheid A., Choppin P. W. 1981; Immunological studies of the function of paramyxovirus glycoproteins. Virology 109:94–105
    [Google Scholar]
  14. Peeples M., Levine S. 1979; Respiratory syncytial virus polypeptides: their location in the virion. Virology 95:137–145
    [Google Scholar]
  15. Walsh E. E., Hruska J. F. 1983; Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. Journal of Virology 47:171–177
    [Google Scholar]
  16. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. Journal of General Virology 65:761–767
    [Google Scholar]
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