1887

Abstract

SUMMARY

Using an ELISA for the detection of virus-specific immune complexes, ten cows were found to be shedding bovine enteric coronavirus. The shedding patterns from five of these animals were followed for a period of 12 weeks, and all were found to be chronically shedding virus. Despite the presence of both faecal and serum antibody the infection was not cleared; therefore, the role of cell-mediated immunity (CMI) was investigated by immunosuppressing the chronically shedding cows with dexamethasone. No major role for CMI in maintaining the chronic infection could be determined, although immunosuppression did result in a temporary reduction in the shedding of virus-specific immune complexes.

Keyword(s): antibody , coronavirus and cows
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1489
1985-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071489.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1489&mimeType=html&fmt=ahah

References

  1. Alexander D. J., Gough R. E., Pattison M. 1978; A long term study of the pathogenesis of infection of chickens with three strains of avian infectious bronchitis virus. Research in Veterinary Science 24:228–235
    [Google Scholar]
  2. Bailey O. T., Pappenheimer A. M., Cheever F. S., Daniels J. B. 1949; A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. Journal of Experimental Medicine 90:195–212
    [Google Scholar]
  3. Bielefeldt Ohmann H., Babiuk L. A. 1984a; Effect of bovine recombinant alpha interferon on inflammatory response of bovine phagocytes. Journal of Interferon Research 4:249–263
    [Google Scholar]
  4. Bielefeldt Ohmann H., Babiuk L. A. 1984b; In vitro generation of hydrogen peroxide and of superoxide by bovine polymorphonuclear neutrophilic granulocytes, blood monocytes and alveolar macrophages. Inflammation 8:251–275
    [Google Scholar]
  5. Bielefeldt Ohmann H., Filion L. G., Babiuk L. A. 1983; Bovine monocytes and macrophages: an accessory role in suppressor cell generation by Con A and in lectin-induced proliferation. Immunology 50:189–199
    [Google Scholar]
  6. Chretien J. H., Garagusi V. F. 1972; Corticosteroid effect on phagocytosis and NBT reduction by human polymorphonuclear neutrophils. Journal of the Reticuloendothelial Society 11:358–367
    [Google Scholar]
  7. Claman H. N. 1975; How corticosteroids work. Journal of Allergy and Clinical Immunology 55:145–151
    [Google Scholar]
  8. Crabtre G. R., Gillis S., Smith K. A., Munck A. 1980; Mechanisms of glucocorticoid-induced immunosuppression: inhibitory effects on expression of the receptors and production of T-cell growth factor. Journal of Steroid Biochemistry 12:445–449
    [Google Scholar]
  9. Crouch C. F., Acres S. D. 1984; Prevalence of rotavirus and coronavirus antigens in feces of normal cows. Canadian Journal of Comparative Medicine 48:340–342
    [Google Scholar]
  10. Crouch C. F., Raybould T. J. G. 1983; Comparison of different antigen preparations as substrates for use in passive haemagglutination and enzyme-linked immunosorbent assays for the detection of antibody against bovine enteric coronavirus. Journal of Clinical Microbiology 18:146–149
    [Google Scholar]
  11. Crouch C. F., Raybould T. J. G., Acres S. D. 1984; Monoclonal antibody capture-enzyme-linked immunosorbent assay for the detection of bovine enteric coronavirus. Journal of Clinical Microbiology 19:388–393
    [Google Scholar]
  12. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay. III. Quantitation of specific antibodies by enzyme labelled anti-immunoglobulin in antigen coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  13. Fauci A. S. 1980; Glucocorticoid effects on circulatory human mononuclear cells. Journal of the Reticuloendothelial Society 27:727–738
    [Google Scholar]
  14. Gielkens A. L. F., Ellens D. J. 1981; Application of purified 5-aminosalicylic acid as substrate in enzyme-linked immunosorbent assay (ELISA). In Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea (Current Topics in Veterinary Medicine and Animal Science, vol. 13) pp 33–39 Edited by de Leeuw P. W., Guinee P. A. M. Boston: Martinus Nijhoff;
    [Google Scholar]
  15. Lai Y. L., Jacoby R. O., Bhatt P. N., Jonas A. M. 1976; Keratoconjunctivitis associated with sialodacryoadenitis in rats. Investigative Ophthalmology 15:538–541
    [Google Scholar]
  16. Langpap T. J., Bergeland M. E., Reed D. E. 1979; Coronaviral enteritis of young calves: virologic and pathologic findings in naturally occurring infections. American Journal of Veterinary Research 40:1476–1478
    [Google Scholar]
  17. Marsolais G., Assaf R., Montpetit C., Marois P. 1978; Diagnosis of viral agents associated with neonatal calf diarrhea. Canadian Journal of Comparative Medicine 42:168–171
    [Google Scholar]
  18. Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. 1973; Pathology of neonatal calf diarrhea induced by a corona-like agent. Veterinary Pathology 10:45–64
    [Google Scholar]
  19. Mengeling W. L., Cutlip R. C. 1976; Pathogenicity of field isolates of hemagglutinating encephalomyelitis virus for neonatal pigs. Journal of the American Veterinary Medical Association 168:236–239
    [Google Scholar]
  20. Moore B., Lee P., Hewish M., Dixon B., Mukherjee T. 1977; Coronaviruses in a training centre for intellectually retarded. Lancet i:261
    [Google Scholar]
  21. Morin M., Lariviere S., Lallier R. 1976; Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea. Canadian Journal of Comparative Medicine 40:228–240
    [Google Scholar]
  22. Raybould T. J. G., Chantler S. 1979; Comparison of activation and blocking procedures in the use of defined antigen substrate spheres (DASS) for quantitative and visual assessment of conjugate activity. Journal of Immunological Methods 27:309–318
    [Google Scholar]
  23. Rinehart J. J., Wuest D., Ackerman G. A. 1982; Corticosteroid alteration of human monocyte to macrophage differentiation. Journal of Immunology 129:1436–1440
    [Google Scholar]
  24. Rodak L., Babiuk L. A., Acres S. D. 1982; Detection by radioimmunoassay and enzyme-linked immunosorbent assay of coronavirus antibodies in bovine serum and lacteal secretions. Journal of Clinical Microbiology 16:34–40
    [Google Scholar]
  25. Roth J. A., Kaeberle M. L. 1981; Effects of in vivo dexamethasone administration on in vitro bovine polymorphonuclear leukocyte function. Infection and Immunity 33:434–441
    [Google Scholar]
  26. Roth J. A., Kaeberle M. L., Hsu W. H. 1982; Effects of ACTH administration on bovine polymorphonuclear leukocyte function and lymphocyte blastogenesis. American Journal of Veterinary Research 43:412–416
    [Google Scholar]
  27. Scherrer R., Corthier G., L’Haridon R. 1981; ELISA for the detection of rotavirus and rotavirus/antibody complexes in faeces. In Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea (Current Topics in Veterinary Medicine and Animal Science, vol. 13) pp 40–48 Edited by de Leeuw P. W., Guinee P. A. M. Boston: Martinus Nijhoff;
    [Google Scholar]
  28. Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cinningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Mcnaughton M. R., Mcintosh K. 1978; Coronaviridae: second report. Intervirology 10:321–328
    [Google Scholar]
  29. Underdahl R., Mebus C. A., Torres-Medina A. 1975; Recovery of transmissible gastroenteritis virus from chronically infected experimental pigs. American Journal of Veterinary Research 36:1473–1476
    [Google Scholar]
  30. Voller A., Bidwell D. 1975; A simple method for detecting antibodies to rubella. British Journal of Experimental Pathology 56:338–339
    [Google Scholar]
  31. Vonderfecht S. L., Osburn B. I. 1982; Immunity to rotavirus in conventional neonatal calves. Journal of Clinical Microbiology 16:935–942
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1489
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1489
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error