1887

Abstract

Summary

Cultured human fibroblasts showed a typical fibrillar organization of microtubules in immunofluorescence, including the vimentin type of intermediate filament as well as actin-containing microfilaments. During infection with herpes simplex virus type 1 (HSV-1), the vimentin organization was maintained whereas actin, myosin and tubulin showed a progressive association with the viral glycoproteins within juxtanuclear structures. These structures could also be revealed with fluorochrome-coupled wheat germ agglutinin. Disruption of the microtubules by demecolcine treatment or their stabilization by taxol treatment did not prevent the aggregation of viral proteins in the cytoplasm. Taxol stabilization of the microtubules allowed the juxtanuclear accumulation of the glycoproteins in HSV-infected cells whereas treatment with demecolcine led to an accumulation of the glycoproteins either in small vesicles in the cytoplasm or in the focal adhesion areas of the cells. Production of infectious intracellular virus particles was reduced in cells treated with demecolcine or with taxol before and during infection. The results of this study indicate that the normal intracellular transport and distribution of the HSV glycoproteins and the formation of infectious virus are dependent on the presence of intact microtubules.

Keyword(s): cytoskeleton , HSV-1 and morphogenesis
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1986-01-01
2024-03-28
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References

  1. Badley R. A., Lloyd C. W., Woods A., Carruthers L., Allcock C., Rees D. A. 1978; Mechanisms of cellular adhesion. III. Preparation and preliminary characterization of adhesion. Experimental Cell Research 117:231–244
    [Google Scholar]
  2. Bedows E., Welsh M. J. 1983; Fate of microfilaments in Vero cells infected with measles virus and herpes simplex virus type 1. Molecular and Cellular Biology 3:712–719
    [Google Scholar]
  3. Ben-Ze’ev A., Abulafia R., Bratosin S. 1983; Herpes simplex virus and protein transport are associated with the cytoskeletal framework and the nuclear matrix in infected BSC-1 cells. Virology 129:501–507
    [Google Scholar]
  4. Braun D. K., Pereira L., Norrild B., Roizman B. 1983; Application of denatured, electrophoretically separated, and immobilized lysate of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins. Journal of Virology 46:103–112
    [Google Scholar]
  5. Debrabander M., Geuens G., Nuydens R., Willebrords R., Demay J. 1981; Taxol induces the assembly of free microtubules in living cells and blocks the organizing capacity of the centrosomes and kinetochores. Proceedings of the National Academy of Sciences, U.S.A 78:5608–5612
    [Google Scholar]
  6. Dix R. D., Courtney R. J. 1976; Effects of cytochalasin B on herpes simplex virus type 1 replication. Virology 70:127–135
    [Google Scholar]
  7. Dustin P. 1978 Microtubules Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  8. Fagraeus A., Tyrrell D. L. J., Norberg R., Norrby E. 1978; Actin filaments in paramyxovirus-infected human fibroblasts studied by indirect immunofluorescence. Archives of Virology 57:291–296
    [Google Scholar]
  9. Geiger B. 1981; Involvement of vinculin in contact-induced cytoskeletal interactions. Cold Spring Harbor Symposia on Quantitative Biology 46:671–682
    [Google Scholar]
  10. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. J. 1982; Evidence for an interaction between the membrane protein of a paramyxovirus and actin. Journal of Virology 42:963–968
    [Google Scholar]
  11. Harboe N., Ingild A. 1973; Immunization, isolation of immunoglobulins, estimation of antibody titers. Scandinavian Journal of Immunology 2: (supplement 1) 161–164
    [Google Scholar]
  12. Heeg U., Haase W., Brauer D., Falke D. 1981; Microtubules and microfilaments in HSV-infected rabbit- kidney cells. Archives of Virology 70:233–246
    [Google Scholar]
  13. Hiller G., Jungwirth C., Weber K. 1981; Fluorescence microscopical analysis of the life cycle of vaccinia virus in chick embryo fibroblasts. Experimental Cell Research 132:81–87
    [Google Scholar]
  14. Howard J. M., Eckert B. S., Bourguignon L. Y. W. 1983; Comparison of cytoskeletal organization in canine distemper virus-infected and uninfected cells. Journal of General Virology 64:2379–2385
    [Google Scholar]
  15. Johnson D. C., Spear P. G. 1982; Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface and the egress of virions from infected cells. Journal of Virology 43:1102–1112
    [Google Scholar]
  16. Lehto V. -P., Virtanen I., Kurki P. 1978; Intermediate filaments anchor the nuclei in nuclear monolayers of cultured fibroblasts. Nature, London 272:175–177
    [Google Scholar]
  17. Menko A. S., Toyama Y., Boettiger D., Holzer H. 1983; Altered cell spreading in cytochalasin B: a possible role for intermediate filaments. Molecular and Cellular Biology 3:113–125
    [Google Scholar]
  18. Meyer R. K., Burger M. M., Tschannen R., Schafer R. 1981; Actin filament bundles in vaccinia virus infected fibroblasts. Archives of Virology 67:11–18
    [Google Scholar]
  19. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  20. Murti K. G., Goorha R. 1983; Interaction of frog virus-3 with the cytoskeleton. I. Altered organization of microtubules, intermediate filaments, and microfilaments. Journal of Cell Biology 96:1248–1257
    [Google Scholar]
  21. Norrild B., Vestergaard B. F. 1977; Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel. Journal of Virology 22:113–117
    [Google Scholar]
  22. Norrild B., Virtanen I., Pedersen B., Pereira L. 1983a; Requirements for transport of HSV-1 glycoproteins to the cell surface membrane of human fibroblasts and Vero cells. Archives of Virology 77:155–166
    [Google Scholar]
  23. Norrild B., Virtanen I., Lehto V. -P., Pedersen B. 1983b; Accumulation of herpes simplex virus type 1 glycoprotein D in adhesion areas of infected cells. Journal of General Virology 64:2499–2503
    [Google Scholar]
  24. Osborn M., Weber K. 1977; The detergent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles. Experimental Cell Research 106:339–349
    [Google Scholar]
  25. Osborn M., Geisler N., Shaw G., Sharp G., Weber K. 1981; Intermediate filaments. Cold Spring Harbor Symposia on Quantitative Biology 46:413–429
    [Google Scholar]
  26. Pereira L., Klassen T., Baringer J. R. 1980; Type-common and type-specific monoclonal antibodies to herpes simplex virus type 1. Infection and Immunity 29:724–732
    [Google Scholar]
  27. Pollard T. D., Fujiwara K., Niedermann R., Maupin-Szamier P. 1976 In Cell Motility (Cold Spring Harbor Conferences on Cell Proliferation, vol. 3) pp 689–724 Edited by Goldman D. R., Pollard T. D., Rosenbaum J. L. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Quinlan M. P., Knipe D. M. 1983; Nuclear localization of herpesvirus proteins: potential role for the cellular framework. Molecular and Cellular Biology 3:315–324
    [Google Scholar]
  29. Rogalski A. A., Singer S. I. 1984; Associations of elements of the Golgi apparatus with microtubules. Journal of Cell Biology 99:1092–1100
    [Google Scholar]
  30. Rogalski A. A., Bergmann J. E., Singer S. J. 1984; Effect of microtubule assembly status on the intracellular processing and surface expression of an integral protein of the plasma membrane. Journal of Cell Biology 99:1101–1109
    [Google Scholar]
  31. Roizman B., Roane P. R. JR 1961; Studies of the determinant antigens of viable cells. I. A method, and its application in tissue culture studies, for enumeration of killed cells, based on the failure of virus multiplication following injury by cytotoxic antibody and complement. Journal of Immunology 87:714–727
    [Google Scholar]
  32. Rutter G., Mannweiler K. 1977; Alterations of actin-containing structures in BHK21 cells infected with Newcastle disease virus and vesicular stomatitis virus. Journal of General Virology 37:233–242
    [Google Scholar]
  33. Schliwa M. 1984; Mechanisms of intracellular organelle transport. In Cell and Muscle Motility vol 5 pp 1–81 Edited by Dowben R. W., Shay J. W. New York: Plenum Press;
    [Google Scholar]
  34. Sharpe A. H., Chen L. B., Fields B. N. 1982; The interaction of mammalian reoviruses with the cytoskeleton of monkey kidney CV-1 cells. Virology 120:399–411
    [Google Scholar]
  35. Thompson W. C., Wilson L., Purich D. L. 1981; Taxol induces microtubule assembly at low temperature. Cell Motility 1:445–454
    [Google Scholar]
  36. Tilney L. G. 1983; Interaction between actin filaments and membranes gives spatial organization to cells. Modern Cell Biology 2:163–199
    [Google Scholar]
  37. Vestergaard B. F., Norrild B. 1979; Crossed immunoelectrophoretic analysis and viral neutralizing activity of five monospecific antisera against five different herpes virus glycoproteins. In Oncogenesis and Herpesviruses III pp 243–248 Edited by de The G., Henle W., Rapp F. Lyon: IARC Scientific Publications No. 24;
    [Google Scholar]
  38. Virtanen I., Ekblom P., Laurila P. 1980a; Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells. Journal of Cell Biology 85:429–434
    [Google Scholar]
  39. Virtanen I., Lehto V.-P., Lehtonen E., Badley R. A. 1980b; Organization of intermediate filaments in cultured fibroblasts upon disruption of microtubules by cold treatment. European Journal of Cell Biology 23:80–84
    [Google Scholar]
  40. Virtanen I., Lehto V.-P., Lehtonen E., Vartio T., Stenman S., Kurki P., Wagner O., Small J. V., Dahl D., Badley R. A. 1981; Expression of intermediate filaments in cultured cells. Journal of Cell Science 50:45–63
    [Google Scholar]
  41. Virtanen I., Badley R. A., Paasivuo R., Lehto V. P. 1984; Distinct cytoskeletal domains revealed in sperm cells. Journal of Cell Biology 99:
    [Google Scholar]
  42. Weber K., Osborn M. 1981; Microtubule and intermediate filament networks in cells viewed by immunofluorescence microscopy. In Cytoskeletal Elements and Plasma Membrane Organization pp 1–53 Edited by Poste G., Nicolson G. L. Amsterdam: EIsevier/North-Holland;
    [Google Scholar]
  43. Weihing R. R. 1979; The cytoskeleton and plasma membrane. In Methods and Achievements in Experimental Pathology vol 8 pp 42–109 Edited by Jasmin G., Cantin M. Basel: S. Karger;
    [Google Scholar]
  44. Wenske E. A., Bratton M. W., Courtney R. J. 1982; Endo-β-N′-acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC. Journal of Virology 44:241–248
    [Google Scholar]
  45. Winkler M., Dawson G. J., Elizan T. S., Berl S. 1982; Distribution of actin and myosin in rat neuronal cell line infected with herpes simplex virus. Archives of Virology 72:95–103
    [Google Scholar]
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