1887

Abstract

Summary

Three disulphide cross-bridged glycoprotein complexes were immunoprecipitated from purified human cytomegalovirus envelopes using a monoclonal antibody with a specificity for a glycoprotein of mol. wt. 52 × 10. These complexes were isolated by electroelution after polyacrylamide gel electrophoresis (PAGE) under non-reducing conditions. Compositional analysis of each complex by PAGE under reducing conditions showed that at least two distinct complexes,one containing glycoproteins with mol. wt. of 52 × 10 and 95 × 10 and the other with glycoproteins of 52 × 10 and 130 × 10, were present. The results obtained indicated that one of these complexes could also exist as a dimer.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-7-1469
1986-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/7/JV0670071469.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-7-1469&mimeType=html&fmt=ahah

References

  1. Britt W. J. 1984; Neutralizing antibodies detect a disulphide-linked glycoprotein complex within the envelope of human cytomegalovirus. Virology 135:369–378
    [Google Scholar]
  2. Eisenberg R. J., Cerini C. P., Heilman C. J., Joseph A. D., Dietzschold B., Golub E., Long D., Ponce De Leon M., Cohen G. H. 1985; Synthetic glycoprotein D-related peptides protect mice against herpes simplex virus challenge. Journal of Virology 56:1014–1017
    [Google Scholar]
  3. Farrar G. H., Oram J. D. 1984; Characterization of the human cytomegalovirus envelope glycoproteins. Journal of General Virology 65:1991–2001
    [Google Scholar]
  4. Furukawa T., Gonczol E., Starr S., Tolpin M. D., Arbeter A., Plotkin S. A. 1984; HCMV envelope antigens induce both humoral and cellular immunity in guinea pigs. Proceedings of the Society for Experimental Biology and Medicine 175:243–250
    [Google Scholar]
  5. Greenaway P. J., Levine D. 1973; Amino acid compositions of simian virus 40 structural proteins. Biochemical and Biophysical Research Communications 52:1221–1227
    [Google Scholar]
  6. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Law K. M., Wilton-Smith P., Farrar G. H. 1985; A murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex. Journal of Medical Virology 17:255–266
    [Google Scholar]
  9. Middledorp J. M., Jongsma J., The T. J. 1985; Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera. Journal of Virology 54:240–244
    [Google Scholar]
  10. North J. R., Morgan A. J., Thompson L., Epstein M. A. 1982; Purified Epstein-Barr virus M 340, 000 glycoprotein induces potent virus-neutralizing antibodies when incorporated in liposomes. Proceedings of the National Academy of Sciences, U.S.A. 79:7504–7508
    [Google Scholar]
  11. Nowak B., Sullivan C., Sarnow P., Thomas R., Bricout F., Nicolas J. C., Fleckenstein B., Levine A. J. 1984; Characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins. Virology 132:325–338
    [Google Scholar]
  12. Pereira L., Hoffman M., Cremer N. 1982a; Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus infected cell extracts by human sera. Infection and Immunity 36:933–942
    [Google Scholar]
  13. Pereira L., Hoffman M., Gallo D., Cremer N. 1982b; Monoclonal antibodies to human cytomegalovirus. Three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infection and Immunity 36:924–932
    [Google Scholar]
  14. Pereira L., Hoffman M., Tatsuno M., Dondero D. 1984; Polymorphism of human cytomegalovirus glycoproteins characterized by monoclonal antibodies. Virology 139:73–86
    [Google Scholar]
  15. Rasmussen L., Mullenax J., Nelson R., Merigan T. C. 1985; Viral polypeptides detected by a complement-dependent neutralizing murine monoclonal antibody to human cytomegalovirus. Journal of Virology 55:274–280
    [Google Scholar]
  16. Stinski M. F. 1976; Human cytomegalovirus: glycoproteins associated with virions and dense bodies. Journal of Virology 19:594–609
    [Google Scholar]
  17. Talbot P., Almeida J. D. 1977; Human cytomegalovirus: purification of enveloped virions and dense bodies. Journal of General Virology 36345–349
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-7-1469
Loading
/content/journal/jgv/10.1099/0022-1317-67-7-1469
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error