1887

Abstract

SUMMARY

The genome of the prototype strain of coxsackievirus B4 (J.V.B. Benschoten) has been cloned in and its complete nucleotide sequence determined. Excluding the poly(A) tract, the RNA genome is 7395 nucleotides in length and appears to encode a single polyprotein of 2183 amino acids. The predicted amino acid sequence of the polyprotein shows close homology (88%) to that of the previously sequenced coxsackievirus B3 and to certain regions of the polyproteins of the polioviruses and human rhinovirus 14. This allows identification of putative polyprotein cleavage signals, antigenic domains and other structural features likely to be important to the biological integrity of the virus.

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1987-07-01
2024-04-25
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References

  1. Argos P., Kamer G., Nicklen M. J. h., Wimmer E. 1984; Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest a common ancestry of these virus families. Nucleic Acids Research 12:7251–7267
    [Google Scholar]
  2. Barret-Connor E. 1985; Is insulin-dependent diabetes mellitus caused by coxsackievirus B infection? A review of the epidemiologic evidence. Review of Infectious Diseases 7:207–215
    [Google Scholar]
  3. Cann A. J., Stanway G., Hauptmann R., Minor P. D., Schild G. C., Clarke L. D., Mountford R. C., Almond J. W. 1983; Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins. Nucleic Acids Research 11:1267–1281
    [Google Scholar]
  4. Cann A. J., Stanway G., Hughes P. J., Minor P. D., Evans D. M. A., Schild G. C., Almond J. W. 1984; Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine. Nucleic Acids Research 12:7787–7792
    [Google Scholar]
  5. Crowell R. L., Landau B. J., Siak J. S. 1981; Picornavirus receptors in pathogenesis. In Virus Receptors part II pp. 169–184 Lonberg-Holm K., Philipson L. Edited by London: Chapman & Hall;
    [Google Scholar]
  6. Dalldorf G. 1950; The coxsackie viruses. Bulletin of the New York Academy of Medicine 26:329–335
    [Google Scholar]
  7. Evans D. M. A., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V. 1985; Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature; London: 314548–550
    [Google Scholar]
  8. Fellner P. 1979; General organisation and structure of the picornavirus genome. In The Molecular Biology of Picornaviruses pp. 25–48 Perez-Bercoff R. Edited by New York: Plenum Press;
    [Google Scholar]
  9. Frisk G., Fohlman J., Kobbah M., Ewald U., Tuvemo T., Diderholm H., Friman G. 1985; High frequency of coxsackie-B-virus specific IgM in children developing type 1 diabetes during a period of high diabetes morbidity. Journal of Medical Virology 17:219–227
    [Google Scholar]
  10. Grist N. R., Bell E. J., Assaad F. 1978; Enteroviruses in human disease. Progress in Medical Virology 24:114–157
    [Google Scholar]
  11. Hewlett M. J., Florkiewicz R. Z. 1980; Sequence of picornavirus RNAs containing a radioiodinated 5′ linked peptide reveals a conserved 5′ sequence. Proceedings of the National Academy of Sciences, U.S.A 77:303–307
    [Google Scholar]
  12. Hogle J. M., Chow M., Filman D. J. 1985; The three-dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  13. King M. L., Shaikh A., Bidwell D., Voller A., Banatvala J. E. 1983; Coxsackie B virus specific IgM responses in children with insulin dependent (juvenile-onset; type 1) diabetes mellitus. Lancet i:1397–1399
    [Google Scholar]
  14. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., Van Der Werf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature; London: 291547–553
    [Google Scholar]
  15. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  16. Kuhn R. J., Wimmer E. 1987; The replication of picornaviruses. In The Molecular Biology of Positive Stranded RNA Viruses Rowlands D. J., Mahy B. W. J., Mayo M. A. Edited by London & Orlando: Academic Press; in press
    [Google Scholar]
  17. La Monica N., Meriam C., Racaniello V. R. 1986; Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing. Journal of Virology 57:515–525
    [Google Scholar]
  18. Larsen G. R., Semler B. L., Wimmer E. 1981; Stable hairpin structure within the 5′-terminal 85 nucleotides of poliovirus RNA. Journal of Virology 37:328–335
    [Google Scholar]
  19. Larsen G. R., Anderson C. W., Dorner A. J., Semler B. L., Wimmer E. 1982; Cleavage sites within the poliovirus capsid protein precursors. Journal of Virology 41:340–344
    [Google Scholar]
  20. Lindberg A. M., Stalhandske P. O. K., Pettersson U. 1987; Genome of coxsackievirus B3 . Virology 156:50–63
    [Google Scholar]
  21. Melnick J. L. 1985; Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Virology pp. 739–794 Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. Edited by New York: Raven Press;
    [Google Scholar]
  22. Minor P. D. 1980; Comparative biochemical studies of type 3 poliovirus. Journal of Virology 34:73–84
    [Google Scholar]
  23. Minor P. D., Schild G. C., Bootman J., Evans D. M. A., Ferguson M., Reeve P., Spitz M., Stanway G., Cann A. J., Hauptmann R., Clarke L. D., Mountford R. C., Almond J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature; London: 301674–679
    [Google Scholar]
  24. Minor P. d., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  25. Newton S. E., Carroll A. R., Campbell R. O., Clarke B. E., Rowlands D. J. 1985; The sequence of foot-and-mouth disease virus RNA to the 5′ side of the poly(C) tract. Gene 40:331–336
    [Google Scholar]
  26. Pallansch M. A., Kew O. M., Semler B. L., Omilianowski D. R., Anderson C. W., Wimmer E., Rueckert R. 1984; Protein processing map of poliovirus. Journal of Virology 49:873–880
    [Google Scholar]
  27. Pincus S. E., Diamond D. C., Emini E. A., Wimmer E. 1986; Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C. Journal of Virology 57:638–646
    [Google Scholar]
  28. Racaniello V. R., Meriam C. 1987; Polio temperature-sensitive mutant containing a single nucleotide deletion in the 5′-noncoding region of the viral RNA. Virology in press
    [Google Scholar]
  29. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; The structure of a human common cold virus (rhinovirus 14) and its functional relations to other picornaviruses. Nature; London: 317145–153
    [Google Scholar]
  30. Rueckert R. R. 1985; Picornaviruses and their replication. In Virology pp. 705–738 Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. Edited by New York: Raven Press;
    [Google Scholar]
  31. Ryan M. D. 1985 Molecular cloning and nucleotide sequence studies on enterovirus 70 Ph.D. dissertation University of Leicester:
    [Google Scholar]
  32. Sarnow P., Bernstein H. D., Baltimore D. 1986; A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome. Proceedings of the National Academy of Sciences, U.S.A 83:571–575
    [Google Scholar]
  33. Sherry B., Rueckert R. 1985; Evidence for at least two dominant neutralisation antigens on human rhinovirus 14. Journal of Virology 53:137–143
    [Google Scholar]
  34. Skern T., Sommergruber W., Blaas D., Gründler P., Fraundorfer F., Pieler C., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  35. Skern T., Neubauer C., Frasel L., Gründler P., Sommergruber W., Zorn M., Kuechler E., Blaas D. 1987; A neutralizing epitope on human rhinovirus type 2 includes amino acid residues between 153 and 164 of viral capsid protein VP2. Journal of General Virology 68:315–323
    [Google Scholar]
  36. Staden R. 1980; A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Research 8:3673–3694
    [Google Scholar]
  37. Stalhandske P. O. K., Lindberg M., Pettersson U. 1984; Replicase gene of coxsackievirus B3. Journal of Virology 51:742–746
    [Google Scholar]
  38. Stanway G., Cann A. J., Hauptmann R., Hughes P., Clarke L. D., Mountford R. C., Minor P. D., Schild G. C., Almond J. W. 1983; The nucleotide sequence of poliovirus type 3 Leon 12 a1b: comparison with poliovirus type 1. Nucleic Acids Research 11:5629–5643
    [Google Scholar]
  39. Stanway G., Hughes P. J., Mountford R.C., Minor P. D., Almond J. W. 1984a; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
  40. Stanway G., Hughes P. J., Mountford R. C., Reeve P., Minor P. D., Schild G. C., Almond J. W. 1984b; Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon/12a1 b. Proceedings of the National Academy of Sciences, U.S.A 81:1539–1543
    [Google Scholar]
  41. Stanway G., Mountford R. C., Cox S. D. J., Schild G. C., Minor P. D., Almond J. W. 1984c; Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method. Archives of Virology 81:67–78
    [Google Scholar]
  42. Takegami T., Semler B. L., Anderson C. w., Wimmer E. 1983; Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides. Virology 128:33–41
    [Google Scholar]
  43. Toyoda H., Kohara M., Kataoka Y., Suganuma T., Omata T., Imura N., Nomoto A. 1984; Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationships, gene function and antigenic determinants. Journal of Molecular Biology 174:561–586
    [Google Scholar]
  44. Toyoda H., Nicklin M.J.H., Murray M. G., Anderson C. W., Dunn J. J., Studier F.W., Wimmer E. 1986; A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell 45:761–770
    [Google Scholar]
  45. Tracy S., Liu H. L., Chapman N. M. 1985; Coxsackie B3: primary structure of the 5′ non-coding and capsid protein coding regions of the genome. Virus Research 3:263–270
    [Google Scholar]
  46. Yoon J. W., Austin M., Onodera T., Notkins A. L. 1979; Virus-induced diabetes mellitus. Isolation of a virus from the pancreas of a child with diabetic ketoacidosis. New England Journal of Medicine 300:1173–1179
    [Google Scholar]
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