1887

Abstract

Summary

The antigenic relationship between a recently isolated porcine respiratory coronavirus (TLM 83) and transmissible gastroenteritis (TGE) virus of swine was studied by neutralization, immunoblotting and radioimmunoassay (RIA) using TGE virus-specific monoclonal antibodies (MAbs) and polyclonal antibodies specific for both viruses. A complete two-way neutralization activity between the two viruses was found. Immunoblotting revealed cross-reactions between TLM 83 and TGE virus antigens at the level of the envelope protein (E1), the nucleoprotein (N) and the peplomer protein (E2). By virus neutralization assays and RIA with TGE virus-specific MAbs, the presence of similar epitopes in the E1 and N proteins and in the neutralization-mediating antigenic site of the E2 protein were demonstrated. E2 protein-specific MAbs, without neutralizing activity and reacting with antigenic sites B, C and D (previously defined), failed to recognize TLM 83. These results indicated a close antigenic relationship and structural similarity between TLM 83 and TGE viruses and also suggested potential ways of differentiating between the two viruses.

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/content/journal/jgv/10.1099/0022-1317-69-7-1725
1988-07-01
2024-04-20
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References

  1. BERNARDI G. 1971; Chromatography of proteins on hydroxy apatite. Methods in Enzymology 22:325–339
    [Google Scholar]
  2. BOHL E. H. 1981 Transmissible gastroenteritis. Diseases of Swine195–201 Edited by Leman A. D., Glock R. D., Mengeling W. L., Penny R. H. C., Scholl E., Straw B. Ames: Iowa State University Press;
    [Google Scholar]
  3. CALLEBAUT P. E., PENSAERT M. B. 1980; Characterization and isolation of structural polypeptides in haemagglutinating encephalomyelitis virus. Journal of General Virology 48:193–204
    [Google Scholar]
  4. CORREA I., JIMENEZ G., SUNE C, BULLIDO M. J., ENJUANES L. 1988; Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Research in press
    [Google Scholar]
  5. DEBOUCK P., PENSAERT M., COUSSEMENT W. 1981; The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777. Veterinary Microbiology 6:157–165
    [Google Scholar]
  6. DOYLE L. P., HUTCHINGS L. M. 1946; A transmissible gastroenteritis in pigs. Journal of the American Veterinary Medical Association 108:257–259
    [Google Scholar]
  7. ENJUANES L., CORREA I., JIMENEZ G., MELGOSA M. P., BULLIDO M. J. 1987 Critical epitopes in transmissible gastroenteritis virus neutralization. Biochemistry and Biology of Coronaviruses351–363 Edited by Lai M. M. C., Stohlman S. A. New York & London: Plenum Press;
    [Google Scholar]
  8. FURUUCHI S., SHIMIZU Y., KUMAGAI T. 1978/1979; Multiplication of low and high cell culture passaged strains of transmissible gastroenteritis virus in organs of newborn piglets. Veterinary Microbiology 3:169–178
    [Google Scholar]
  9. GARWES D. J., POCOCK D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  10. HAELTERMAN E. O. 1972; On the pathogenesis of transmissible gastroenteritis of swine. Journal of the American Veterinary Medical Association 160:534–540
    [Google Scholar]
  11. HORZINEK M. C, LUTZ H., PEDERSEN N. C. 1982; Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses. Infection and Immunity 37:1148–1155
    [Google Scholar]
  12. KEMENY L. J., WILTSEY V. L., RILEY J. L. 1975; Upper respiratory infection of lactating sows with transmissible gastroenteritis virus following contact exposure to infected piglets. Cornell Veterinarian 65:352–362
    [Google Scholar]
  13. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. LAUDE H., CHAPSAL J.-M., GELFI J., LABIAU S., GROSCLAUDE J. 1986; Antigenic structure of monoclonal antibodies directed against virion proteins. Journal of General Virology 67:119–130
    [Google Scholar]
  15. PENSAERT M. B., CALLEBAUT P. E. 1974; Characteristics of a coronavirus causing vomition and wasting in pigs. Archiv für die gesamte Virusforschung 44:35–40
    [Google Scholar]
  16. PENSAERT M. B., DEBOUCK P., REYNOLDS D. J. 1981; An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses. Archives of Virology 68:45–62
    [Google Scholar]
  17. PENSAERT M., CALLEBAUT P., VERGOTE J. 1986; Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Veterinary Quarterly 8:257–261
    [Google Scholar]
  18. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  19. VOETS M. T., PENSAERT M., RONDHUIS P. R. 1980; Vaccination of pregnant sows against transmissible gastroenteritis using two attenuated virus strains and different inoculation routes. Veterinary Quarterly 2:211–219
    [Google Scholar]
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