1887

Abstract

SUMMARY

An infectious recombinant human adenovirus type 5 (Ad5) vector, AdG12, which carries the glycoprotein gene of vesicular stomatitis virus (VSV) and expresses that gene in cultured HeLa cells was used to examine the host range of insert expression by human Ad vectors. The VSV glycoprotein was expressed in bovine, canine and murine cells when infected with AdG 12 in culture. These cell lines are respectively permissive, non-permissive and semi-permissive for human Ad5 replication. Administration of the AdG 12 vector to calves, piglets or dogs by either the subcutaneous or oral route resulted in the production of high titres of neutralizing antibodies to VSV. Mice injected intraperitoneally with the vector produced neutralizing antibodies and were protected against subsequent intravenous challenge with normally lethal doses of VSV. This work demonstrates the utility of human adenoviral vectors for antigen expression in a number of non-human cell lines and for the induction of an immune response to the delivered antigen in a number of species.

Keyword(s): adenovirus , vectors and VSV

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/content/journal/jgv/10.1099/0022-1317-70-2-429
1989-02-01
2024-03-28
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References

  1. Ballay A., Levrero M., Buendia M. A., Tiollais P., Perricaudet M. 1985; In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses. EMBO Journal 4:3861–3865
    [Google Scholar]
  2. Davidson D., Hassell J. A. 1987; Overproduction of polyomavirus middle T antigen in mammalian cells through the use of an adenovirus vector. Journal of Virology 61:1226–1239
    [Google Scholar]
  3. Haj-Ahmad Y., Graham F. L. 1986; Development of a helper-independent human adenovirus vector and its use in the transfer of the herpes simplex virus thymidine kinase gene. Journal of Virology 57:267–274
    [Google Scholar]
  4. Johnson D. C., Ghosh-Choudhury G., Smiley J. R., Fallis L., Graham F. L. 1988; Abundant expression of herpes simplex virus glycoprotein gB using an adenovirus vector. Virology 163:1–14
    [Google Scholar]
  5. Jones N., Shenk T. 1979; Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell 16:683–689
    [Google Scholar]
  6. Kang C. Y., Prevec L. 1969; Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. Journal of Virology 3:404–413
    [Google Scholar]
  7. Morin J. E., Lubeck M. D., Barton J. E., Conley A. J., Davis A. R., Hang P. P. 1987; Recombinant adenovirus induces antibody response to hepatitis B virus surface antigen in hamsters. Proceedings of the National Academy of SciencesU.S.A 84:4626–4630
    [Google Scholar]
  8. Nichol S. T. 1987; Molecular epizootiology and evolution of vesicular stomatitis virus New Jersey. Journal of Virology 61:1029–1036
    [Google Scholar]
  9. Nichol S. T. 1988; Genetic diversity of enzootic isolates of vesicular stomatitis virus New Jersey. Journal of Virology 62:572–579
    [Google Scholar]
  10. Prevec L., Kang C. Y. 1970; Homotypic and heterotypic interference by defective particles of vesicular stomatitis virus. Nature, London 228:25–27
    [Google Scholar]
  11. Rosenthal K. L., Oldstone M. B. A., Hengartner H., Zinkernagel R. M. 1983; Specificity of in vitro cytotoxic T cell clones directed against vesicular stomatitis virus. Journal of Immunology 131:475–478
    [Google Scholar]
  12. Ruether J. E., Maderious A., Lavery D., Logan J., Fu S. M., Chen-Kiang S. 1986; Cell-type-specific synthesis of murine immunoglobulin mu RNA from an adenovirus vector. Molecular and Cellular Biology 6:123–133
    [Google Scholar]
  13. Schneider M., Graham F. L., Prevec L. 1989; Expression of the glycoprotein of vesicular stomatitis virus by infectious adenovirus vectors. Journal of General Virology 70:417–427
    [Google Scholar]
  14. Smith J. S., Reid-Sanden F. L., Roumillat L. F., Trimarchi C., Clark K., Baer G. M., Winkler W. G. 1986; Demonstration of antigenic variation among rabies virus isolates by using monoclonal antibodies to nucleocapsid proteins. Journal of Clinical Microbiology 24:573–580
    [Google Scholar]
  15. Tremblay M. L., Yee S.-P., Persson R. H., Bacchetti S., Smiley J. R., Branton P. E. 1985; Activation and inhibition of expression of the 72,000-Da early protein of adenovirus type 5 in mouse cells constitutively expressing an immediate early protein of herpes simplex virus type 1. Virology 144:35–45
    [Google Scholar]
  16. Webster W. A., Casey G. A., Charlton K. M. 1986; Major antigenic groups of rabies virus in Canada determined by anti-nucleocapsid monoclonal antibodies. Comparative Immunology, Microbiology and Infectious Diseases 9:59–69
    [Google Scholar]
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