1887

Abstract

The complete nucleotide sequence of the S RNA of tomato spotted wilt virus (TSWV) was determined. The RNA is 2916 nucleotides long and has an ambisense coding strategy. The sequence contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted of 52·4K and one in the viral complementary sense which encodes the viral nucleocapsid protein of 28·8K. Both proteins are expressed by translation of two subgenomic RNA species that possibly terminate at a long stable hairpin structure, located at the intergenic region. The structure of this RNA segment resembles that of the arthropod-borne phleboviruses (family Bunyaviridae). The absence of significant sequence homology between TSWV and bunyaviruses infecting animals suggests that TSWV should be considered as a representative of a new genus within the Bunyaviridae.

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1990-05-01
2024-04-19
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References

  1. Auperin D. D., Romanowski V., Galinski M., Bishop D. H. L. 1984; Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, ambisense viral S RNA. Journal of Virology 52:897–904
    [Google Scholar]
  2. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  3. Bernstein J. M., Hruska J. F. 1981; Respiratory syncytial virus proteins: identification by immunoprecipitation. Journal of Virology 38:278–285
    [Google Scholar]
  4. Clegg J, Oram J. D. 1985; Molecular cloning of Lassa virus RNA: nucleotide sequence and expression of the nucleocapsid protein gene. Virology 144:363–372
    [Google Scholar]
  5. De Haan P., Wagemakers L., Goldbach R., Peters D. 1989a; Tomato spotted wilt virus, a new member of the Bunyaviridae?. In Genetics and Pathogenicity of Negative Strand Viruses pp 287–290 Kolakofsky D., Mahy B. W. J. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  6. De Haan P., Wagemakers L., Peters D., Goldbach R. 1989b; Molecular cloning and terminal sequence determination of the S and M RN As of tomato spotted wilt virus. Journal of General Virology 70:3469–3473
    [Google Scholar]
  7. De Vries S. C., Springer J., Wessels J. G. H. 1982; Diversity of abundant mRNA sequences and patterns of protein synthesis in etiolated and greened pea seedlings. Planta 156:129–135
    [Google Scholar]
  8. Emery V. C., Bishop D. H. L. 1987; Characterization of Punta Toro S mRNA species and identification of an inverted complementary sequence in the intergenic region of Punta Toro phlebovirus S RNA that is involved in mRNA transcription termination. Virology 156:1–11
    [Google Scholar]
  9. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences U.S.A.: 783824–3828
    [Google Scholar]
  10. Ie T. S. 1970; Tomato spotted wilt virus.. CMIjAAB Descriptions of Plant Viruses39
    [Google Scholar]
  11. Ihara T., Akashi H., Bishop D. H. L. 1984; Novel coding strategy (ambisense genomic RNA) revealed by sequence analysis of Punta Toro phlebovirus S RNA. Virology 136:293–306
    [Google Scholar]
  12. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Habor Laboratory;
    [Google Scholar]
  14. Marriott A. C., Ward V. K., Nuttall P. A. 1989; The S RNA segment of sandfly fever Sicilian virus: evidence for an ambisense genome. Virology 169:341–345
    [Google Scholar]
  15. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  16. Milne R. G., Francki R. I. B. 1984; Should tomato spotted wilt virus be considered as a possible member of the family Bunyaviridae?. Intervirology 22:72–76
    [Google Scholar]
  17. Mohamed N. A. 1981; Isolation and characterization of subviral structures from tomato spotted wilt virus. Journal of General Virology 53:197–206
    [Google Scholar]
  18. Mohamed N. A., Randles J. W., Francki R. I. B. 1973; Protein composition of tomato spotted wilt virus. Virology 56:12–21
    [Google Scholar]
  19. Paliwal Y. C. 1974; Some properties and thrip transmission of tomato spotted wilt virus in Canada. Canadian Journal of Botany 52:1177–1182
    [Google Scholar]
  20. Romanowski V., Atsura Y., Bishop D. H. L. 1985; Complete sequence of the S RNA of lymphocytic choriomeningitis virus (WE strain) compared to that of Pichinde arenavirus. Virus Research 3:101–114
    [Google Scholar]
  21. Sakimura K. 1962; The present status of thrips-borne viruses. In Biological Transmission of Disease Agents pp 33–40 Maramorosch K. Edited by New York: Academic Press;
    [Google Scholar]
  22. Salvato M. 1989; Ambisense nature of the L genomic segment of LCMV. In Genetics and Pathogenicity of Negative Strand Viruses pp 168–173 Kolakofsky D., Mahy B. W. J. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  24. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  25. Strauss J. H., Strauss E. G. 1988; Evolution of RNA viruses. Annual Review of Microbiology 42:657–683
    [Google Scholar]
  26. Tas P. W. L., Boerjan M. L., Peters D. 1977; The structural proteins of tomato spotted wilt virus. Journal of General Virology 36:267–279
    [Google Scholar]
  27. Van Den Hurk J., Tas P. W. L., Peters D. 1977; The ribonucleic acid of tomato spotted wilt virus. Journal of General Virology 36:81–91
    [Google Scholar]
  28. Verkleij F. N., Peters D. 1983; Characterization of a defective form of tomato spotted wilt virus. Journal of General Virology 64:677–686
    [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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