1887

Abstract

The complete nucleotide sequence of the simian virus 41 (SV41) large (L) protein gene was determined. The L gene spanned 6883 nucleotides including a putative trailer RNA, and the L mRNA contained a single large open reading frame encoding a polypeptide of 2269 amino acids. Dot-matrix comparisons under stringent conditions identified domains highly conserved among paramyxoviruses. Domain 3 is the most highly conserved, and has been hypothesized to be the RNA polymerase active site. A phylogenetic tree was constructed from the sequences of the L proteins of seven paramyxoviruses. SV41 was most closely related to human parainfluenza virus type 2 (HPIV-2), and SV41, HPIV-2 and SV5 form a subgroup. The intergenic sequences at the nucleocapsid protein-phosphoprotein and haemagglutinin—neuraminidase-L protein gene junctions, and the 5′ trailer sequence of SV41 were also determined, and it was shown that the first 13 nucleotides of the 5′ trailer sequence are complementary to those of the 3′ leader sequence. The intergenic, and gene-start and -end sequences of SV41, HPIV-2 and SV5 are shown.

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1992-10-01
2024-03-29
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References

  1. Blumberg B. M., Crowley J. C., Silverman J. I., Menonna J., Cook S. D., Dowling P. C. 1988; Measles virus L protein evidences elements of ancestral RNA polymerase. Virology 164:487–497
    [Google Scholar]
  2. Elango N., Kovamees J., Varsanyi T. M., Norrby E. 1989; mRNA sequence and deduced amino acid sequence of the mumps virus small hydrophobic protein gene. Journal of Virology 63:1413–1415
    [Google Scholar]
  3. Galinski M. S., Mink M. A., Pons M. W. 1988; Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein. Virology 165:499–510
    [Google Scholar]
  4. Higuchi Y., Miyahara Y., Kawano M., Tsurudome M., Matsu-mura H., Kusagawa S., Komada H., Nishio M., Ito Y. 1992; Sequence analysis of the large (L) protein of simian virus 5. Journal of General Virology 73:1005–1010
    [Google Scholar]
  5. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1990a; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174:308–313
    [Google Scholar]
  6. Kawano M., Bando H., Ohgimoto S., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. 1990b; Complete nucleotide sequence of the matrix gene of human parainfluenza type 2 virus and expression of the M protein in bacteria. Virology 179:857–861
    [Google Scholar]
  7. Kawano M., Okamoto K., Bando H., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1991; Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences. Nucleic Acids Research 19:2739–2776
    [Google Scholar]
  8. Kimura M. 1983; Rate of evolution at the molecular level. Comparative studies of protein sequences. In The Natural Theory of Molecular Evolution pp. 65–76 Cambridge: Cambridge University Press;
    [Google Scholar]
  9. Kondo K., Fujii M., Nakamura T., Bando , Kawano M., Tsurudome M., Komada H., Kusagawa S., Nishio M., Ito Y. 1991; Sequence characterization of the matrix protein genes of parainfluenza virus type 4A and 4B. Journal of General Virology 72:2283–2287
    [Google Scholar]
  10. Miller R. H., Pursell A. R., Mitchell F. E., Johnson K. M. 1964; A newly discovered myxovirus (S.V.41) isolated from cell cultures of cynomolgus monkey kidney. American Journal of Hygiene 80:365–376
    [Google Scholar]
  11. Miyahara K., Kitada S., Yoshimoto M., Matsumura H., Kawano M., Komada H., Tsurudome M., Kusagawa S., Nishio M., Ito Y. 1992; Molecular evolution of human paramyxoviruses–nucleotide sequence analyses of the human parainfluenza type 1 virus NP and M protein genes and construction of phylogenetic trees for all the human paramyxoviruses. Journal of Virology 124:255–268
    [Google Scholar]
  12. Nishio M., Tsurudome M., Bando H., Ito Y. 1990; Immunological relationships of simian virus 41 (SV41) to other paramyxoviruses and serological evidence of SV41 infection in human populations. Journal of General Virology 71:2093–2097
    [Google Scholar]
  13. Poch O., Blumberg B. M., Bougueleret L., Tordo N. 1990; Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. Journal of General Virology 71:1153–1162
    [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  15. Sheshberadaran H., Lamb R. A. 1990; Sequence characterization of the membrane protein gene of paramyxovirus simian virus 5. Virology 176:234–243
    [Google Scholar]
  16. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequence of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  17. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
  18. Tsurudome M., Bando H., Nishio M., Iwamoto Y., Kawano M., Kondo K., Komada H., Ito Y. 1990; Antigenic and structural properties of a paramyxovirus simian virus 41 (SV41) reveal a close relationship with human parainfluenza type 2 virus. Virology 179:738–748
    [Google Scholar]
  19. Tsurudome M., Bando H., Kawano M., Matsumura H., Komada H., Nishio M., Ito Y. 1991a; Transcripts of simian virus 41 (SV41) matrix gene are exclusively dicistronic with the fusion gene which is also transcribed as a monocistron. Virology 184:93–100
    [Google Scholar]
  20. Tsurudome M., Oki N., Higuchi Y., Miyahara K., Yoshimoto M., Mutsuga N., Kitada S., Ogawa M., Miyahara Y., Okamoto K., Kawano M., Komada H., Matsumura H., Kusagawa S., Nishio M., Ito Y. 1991b; Molecular relationships between parainfluenza virus type 2, and simian viruses 41 and 5: determination of nucleoprotein gene sequences of simian viruses 41 and 5. Journal of General Virology 72:2289–2292
    [Google Scholar]
  21. Yuasa T., Bando H., Kawano M., Tsurudome M., Nishio M., Kondo K., Komada H., ITo Y. 1990; Sequence analysis of the 3′ genome end and NP gene of human parainfluenza type 2 virus: sequence variation of the gene-starting signal and the conserved 3′ end. Virology 179:777–784
    [Google Scholar]
  22. Yusoff K., Miller S. N., Chambers P., Emmerson P. T. 1987; Nucleotide sequence analysis of the L gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses. Nucleic Acids Research 15:3961–3976
    [Google Scholar]
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