1887

Abstract

The antigenic sites A and C (the G-H loop and the C terminus, respectively) in VP1 of foot-and-mouth disease virus (FMDV) have been considered the immunodominant regions of the virus involved in the induction of protection. Other antigenic sites have been described but their involvement in protection has not been established. Here we report that two closely related but serologically different FMDVs (the field isolate C Argentina/84 and the vaccine strain C Resende Br/55) have identical A and C sites but differ at other antigenic sites. Such differences have been documented by reactivity with a panel of 28 monoclonal antibodies (MAbs). The two viruses reacted to the same extent with each of 13 MAbs which recognized epitopes within sites A or C, but reacted differently with six out of 15 MAbs that recognized other sites. Accordingly, sequencing of the entire region coding for the capsid proteins, for both viruses, revealed four amino acid substitutions at three antigenic sites other than A and C. The results suggest that identity of sites A and C may not be sufficient to induce cross-protection, and provide the first evidence of significant antigenic diversification of FMDV in the field mediated by amino acid substitutions outside sites A or C.

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1992-12-01
2024-04-23
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References

  1. Acharya R., Fry E., Stuart D., Fox G., Rowlands D., Brown F. 1989; The three-dimensional structure of foot-and-mouth disease virus at 2.9 A resolution. Nature, London 337:709–715
    [Google Scholar]
  2. Alonso Fernandez A., Casas Olascoaga R., Augé de Mello P., Fernandez G., Mazzuca G. 1986; The evolution of the FMDV virus C3 Argentina in the field. Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease pp 42–48 Lanham, Maryland: Beman-Unipub;
    [Google Scholar]
  3. Bergmann I. E., Tiraboschi B., Mazzuca G., Fernandez E., Michailoff C. A., Scodeller E. A., La Torre J. L. 1988; Serological and biochemical analysis of foot-and-mouth disease virus (serotype C3) isolated in Argentina between 1981 and 1986. Vaccine 6:245–252
    [Google Scholar]
  4. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J, Brown F. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 298:30–33
    [Google Scholar]
  5. Bolwell C., Clarke B. E., Parry N. R., Ouldridge E. J., Brown F., Rowlands D. J. 1989; Epitope mapping of foot-and-mouth disease virus with neutralizing monoclonal antibodies. Journal of General Virology 70:59–68
    [Google Scholar]
  6. Bolwell C., Parry N. R., Rowlands D. J. 1992; Comparison between in vitro neutralization titres and in vivo protection against homologous and heterologous challenge induced by vaccines prepared from two serologically distinct variants of foot-and-mouth disease virus, serotype A22. Journal of General Virology 73:727–731
    [Google Scholar]
  7. Clarke B. E., Carroll A. R., Rowlands D. J., Nicholson B. H., Houghten R. A., Lerner R. A., Brown F. 1983; Synthetic peptides mimic subtype specificity of foot-and-mouth disease virus. FEBS Letters 157:261–264
    [Google Scholar]
  8. Doel T. R., Gale C., Do Amaral C. M. C. F., Mulcahy G., Di Marchi R. 1990; Heterotypic protection induced by synthetic peptides corresponding to three serotypes of foot-and-mouth disease virus. Journal of Virology 64:2260–2264
    [Google Scholar]
  9. Domingo E., Davila M., Ortin J. 1980; Nucleotide sequence heterogeneity of the RNA from a natural population of foot-and-mouth disease virus. Gene 11:333–346
    [Google Scholar]
  10. Domingo E., Mateu M. G., Martínez M. A., Dopazo J., Moya A., Sobrino F. 1990; Genetic variability and antigenic diversity of foot-and-mouth disease virus. In Applied Virology Research: Virus Variability, Epidemiology and Control vol 2 pp. 233–266 Edited by Kurstak E., Marusyk R. G., Murphy F. A., Van Regenmortel M. H. V. New York: Plenum Press;
    [Google Scholar]
  11. Domingo E., Escarmis C., Martinez M. A., Martínez-Salas E., Mateu M. G. 1992; Foot-and-mouth disease virus populations are quasispecies. Current Topics in Microbiology and Immunology 176:33–47
    [Google Scholar]
  12. Giavedoni L. D., Kaplan G., Marcovecchio F., Piccone M. E., Palma E. L. 1991; Protection conferred by TrpE fusion proteins containing portions of the C-terminal region of capsid protein VP 1 of foot-and-mouth disease virus. Journal of General Virology 72:967–971
    [Google Scholar]
  13. Kitson J. D. A., McCahon D., Belsham G. J. 1990; Sequence analysis of monoclonal antibody resistant mutants of type O foot-and-mouth disease virus: evidence for the involvement of the three surface exposed capsid proteins in four antigenic sites. Virology 179:26–34
    [Google Scholar]
  14. Kitson J. D. A., Burke K. L., Pullen L. A., Belsham G. J., Almond J. W. 1991; Chimeric polioviruses that include sequences derived from two independent antigenic sites of foot-and-mouth disease virus (FMDV) induce neutralizing antibodies against FMDV in guinea pigs. Journal of Virology 65:3068–3075
    [Google Scholar]
  15. Martínez M. A., Carrillo C., Plana J., Mascarella R., Bergada J., Palma E. L., Domingo E., Sobrino F. 1988; Genetic and immunogenic variations among closely related isolates of foot-and-mouth disease virus. Gene 62:75–84
    [Google Scholar]
  16. Martínez M. A., Hernandez J., Piccone M. E., Palma E. L., Domingo E., Knowles N. J., Mateu M. G. 1991; Two mechanisms of antigenic diversification of foot-and-mouth disease virus. Virology 184:695–706
    [Google Scholar]
  17. Martínez M. A., Dopazo J., Hernandez J., Mateu M. G., Sobrino F., Domingo E., Knowles N. J. 1992; Evolution of the capsid protein genes of foot-and-mouth disease virus: antigenic variation without accumulation of amino acid substitutions over six decades. Journal of Virology 66:3557–3565
    [Google Scholar]
  18. Mateu M. G., Rocha E., Vicente O., Vayreda F., Navalpotro C., Andreu A., Pedroso E., Giralt E., Enjuanes L., Domingo E. 1987; Reactivity with monoclonal antibodies of viruses from an episode of foot-and-mouth disease. Virus Research 8:261–274
    [Google Scholar]
  19. Mateu M. G., Da Silva J. L., Rocha E., De Brum D. L., Alonso A., Enjuanes L., Domingo E., Barahona H. 1988; Extensive antigenic heterogeneity of foot-and-mouth disease virus of serotype C. Virology 167:113–124
    [Google Scholar]
  20. Mateu M. G., Martínez M. A., Rocha E., Andreu D., Parejo J., Giralt E., Sobrino F., Domingo E. 1989; Implications of a quasispecies genome structure: effect of frequent, naturally occurring amino acid substitutions on the antigenicity of foot-and-mouth disease virus. Proceedings of the National Academy of Sciences, U.S.A. 86:5883–5887
    [Google Scholar]
  21. Mateu M. G., Martínez M. A., Capucci L., Andreu D., Giralt E., Sobrino F., Brocchi E., Domingo E. 1990; A single amino acid substitution affects multiple overlapping epitopes in the major antigenic site of foot-and-mouth disease virus of serotype C. Journal of General Virology 71:629–637
    [Google Scholar]
  22. Mateu M. G., Andreu D., Carreno C., Roig X., Cairo J. J., Camarero J. A., Giralt E., Domingo E. 1992; Non-additive effects of multiple amino acid substitutions on antigen-antibody recognition. European Journal of Immunology 22:1385–1389
    [Google Scholar]
  23. Minor P. D. 1990; Antigenic structure of picomaviruses. Current Topics in Microbiology and Immunology 161:121–154
    [Google Scholar]
  24. Ouldridge E. J., Parry N. R., Barnett P. V., Bolwell C., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1986; Comparison of the structures of the major antigenic sites of foot-and-mouth disease viruses of two different serotypes. In Vaccines 86: New Approaches to Immunization pp. 45–50 Edited by Brown F., Chanock R. M., Lerner R. A. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Parry N. R., Barnett P. V., Ouldridge E. J., Rowlands D. J., Brown F. 1989a; Neutralizing epitopes of type O foot-and-mouth disease virus. II. Mapping three conformational sites with synthetic peptide reagents. Journal of General Virology 70:1493–1503
    [Google Scholar]
  26. Parry N. R., Ouldridge E. J., Barnett P. V., Clarke B. E., Francis M. J., Fox J. D., Rowlands D. J., Brown F. 1989b; Serological prospects for peptide vaccines against foot-and-mouth disease virus. Journal of General Virology 70:2919–2930
    [Google Scholar]
  27. Parry N. R., Fox G., Rowlands D. J., Brown F., Fry E., Acharya R., Logan D., Stuart D. 1990; Structural and serological evidence for a novel mechanism of antigenic variation in foot-and-mouth disease virus. Nature, London 347:569–572
    [Google Scholar]
  28. Pereira H. G. 1977; Subtyping of foot-and-mouth disease virus. In Developments in Biological Standardization vol 35 pp. 167–174 Edited by Mackowiak C., Regamey R. H. Basel: S. Karger;
    [Google Scholar]
  29. Pfaff E., Mussgay M., Böhm H. O., Schulz G. E., Schaller H. 1982; Antibodies against a preselected peptide recognize and neutralize foot-and-mouth disease virus. EMBO Journal 1:869–874
    [Google Scholar]
  30. Piccone M. E. 1989 Caracterizacion molecular de cepas del virus de la fiebre aftosa actuantes en Argentina PhD. thesis Universidad de Buenos Aires;
    [Google Scholar]
  31. Piccone M. E., Kaplan G., Giavedoni L., Domingo E., Palma E. L. 1988; VP1 of serotype C foot-and-mouth disease virus: long-term conservation of sequences. Journal of Virology 62:1469–1473
    [Google Scholar]
  32. Rowlands D. J., Clarke B. E., Carroll A. R., Brown F., Nicholson B. H., Bittle J. L., Houghten R. A., Lerner R. A. 1983; Chemical basis of antigenic variation in foot-and-mouth disease virus. Nature, London 306:694–697
    [Google Scholar]
  33. Sobrino F., Martínez M. A., Carrillo C., Beck E. 1989; Antigenic variation of foot-and-mouth disease virus of serotype C during propagation in the field is mainly restricted to only one structural protein (VP1). Virus Research 14:273–280
    [Google Scholar]
  34. Strohmaier K., Franze R., Adam K.-H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  35. Thomas A. M., Woortmeijer R. J., Puijk W., Barteling S. J. 1988; Antigenic sites of foot-and-mouth disease virus type A10. Journal of Virology 62:2782–2789
    [Google Scholar]
  36. Xie Q.-C., McCahon D., Crowther J. R., Belsham G. J., McCullough K. C. 1987; Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate antigenic sites. Journal of General Virology 681637–1647
    [Google Scholar]
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