1887

Abstract

A PCR assay was developed to detect known and as yet unidentified papillomaviruses (PVs). For this purpose we analysed the conserved amino acid sequences in the L1 and E1 open reading frames of 45 human and nine animal PVs. Candidate regions for the design of a primer were identified as those having the least number of amino acid and nucleotide sequence variants among the different PVs. These regions in the L1 ORF have been described previously. We modified the sequences of the backward and the forward primers, as well as the sequence of the oligonucleotide used as the degenerate probe, in order to cover a broader spectrum of PVs. The sensitivity of the assay for the human and animal PVs tested after hybridization with a P-labelled degenerate oligonucleotide probe was one genome copy per cell for integrated PV DNA and 10 genome copies per cell for plasmid PV DNA. The only exceptions were human papillomavirus (HPV) type 4, HPV60 and HPV65, for which a lower sensitivity was obtained. This group could be detected only by using additional primers. The assay was used to analyse 85 lung cancer biopsies representing different histological types. Using this system no PV DNA sequences were detected in the biopsies when compared with human placental DNA (a negative control) and PV DNA-positive standards.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-5-1149
1994-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/5/JV0750051149.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-5-1149&mimeType=html&fmt=ahah

References

  1. Byrne J. C., Tsao M. S., Fraser R. S., Howley P. M. 1987; Human papillomavirus-11 DNA in a patient with chronic laryngotracheal papillomatosis and metastatic squamous cell carcinoma of the lung. New England Journal of Medicine 317:873
    [Google Scholar]
  2. Compton T. 1990; Degenerate primers for DNA amplification. In PCR Protocols: A Guide to Methods and Applications pp 39–45 Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. Edited by San Diego: Academic Press;
    [Google Scholar]
  3. Delius H., Hofmann B. 1994; Primer-directed sequencing of human papillomavirus types. In Current Topics in Microbiology and Immunology, 186 pp 13–31 Zur Hausen H. Edited by Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  4. Dilorenzo T. P., Tamsen A., Abramson A. L., Steinberg B. M. 1992; Human papillomavirus type 6a DNA in the lung carcinoma of a patient with recurrent laryngeal papillomatosis is characterized by a partial duplication. Journal of General Virology 73:423–428
    [Google Scholar]
  5. Fujinaga Y., Shimada M., Okazawa K., Fukushima M., Kato I., Fujinaga K. 1991; Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction. Journal of General Virology 72:1039–1044
    [Google Scholar]
  6. Gregoire L., Arella M., Campione-Piccardo J., Lancaster W. D. 1989; Amplification of human papillomavirus DNA sequences by using conserved primers. Journal of Clinical Micro-biology 27:2660–2665
    [Google Scholar]
  7. Grimmel M., De Villiers E. -M., Neumann C., Pawlita M., Zur Hausen H. 1988; Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas. International Journal of Cancer 41:5–9
    [Google Scholar]
  8. Guillon L., Sahli R., Chaubert P., Monnier P., Cuttat J. F., Costa J. 1991; Squamous cell carcinoma of the lung in a nonsmoking, nonirradiated patient with juvenile laryngotracheal papillomatosis. Evidence of human papillomavirus-11 DNA in both carcinoma and papillomas. American Journal of Surgical Pathology 15:891–898
    [Google Scholar]
  9. Helmuth R. A., Strate R. W. 1987; Squamous carcinoma of the lung in a nonirradiated, nonsmoking patient with juvenile laryngotracheal papillomatosis. American Journal of Surgical Pathology 11:643
    [Google Scholar]
  10. Innis M. A., Gelfand D. H. 1990; Optimization of PCRs. In PCR Protocols: A Guide to Methods and Applications pp 1–12 Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. Edited by San Diego: Academic Press;
    [Google Scholar]
  11. Manos M. M., Ting Y., Wright D. K., Lewis A. J., Broker T. R., Wolinsky S. M. 1989; The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells 7:209–214
    [Google Scholar]
  12. Ostrow R. S., Manias D. A., Fong W. J., Zachow K. R., Faras A. J. 1987; A survey of human cancers for human papillomavirus DNA by filter hybridization. Cancer 59:429–434
    [Google Scholar]
  13. Popper H. H., Wirnsberger G., Juettner-Smolle F. M., Pongratz M. G., Sommersgutter M. 1992; The predictive value of human papillomavirus (HPV) typing in the prognosis of bronchial squamous cell papillomas. Histopatkology 21:323–330
    [Google Scholar]
  14. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., Zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical car-cinoma cells. Nature; London: 314111–114
    [Google Scholar]
  15. Snijders P. J. F., Van Den Brule A. J. C., Schrijnemakers H. F. J., Snow G., Meijer C. J. L. M., Walboomers J. M. M. 1990; The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. Journal of General Virology 71:173–181
    [Google Scholar]
  16. Snijders P. J. F., Meijer C. J. L. M., Walboomers J. M. M. 1991; Degenerate primers based on highly conserved regions of amino acid sequence in papillomaviruses can be used in a generalized polymerase chain reaction to detect productive human papillomavirus infections. Journal of General Virology 72:2781–2786
    [Google Scholar]
  17. Stremlau A., Gissmann L., Ikenberg H., Stark M., Bannasch P., Zur Hausen H. 1985; Human papillomavirus type 16 related DNA in an anaplastic carcinoma of the lung. Cancer 55:1737–1740
    [Google Scholar]
  18. Syrjänen K. J. 1980; Bronchial squamous cell carcinomas associated with epithelial changes identical to condylomatous lesions of the uterine cervix. Lung 158:131–142
    [Google Scholar]
  19. Syrjänen K. J., Syrjänen S. M. 1987; Human papillomavirus DNA in bronchial squamous cell carcinomas. Lancet i:168–169
    [Google Scholar]
  20. Van Den Brule A. J. C., Snijders P. J. F., Gordijn R. L. J., Bleker O. P., Meijer C. J. L. M., Walboomers J. M. M. 1990; General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas. International Journal of Cancer 45:644–649
    [Google Scholar]
  21. Wagatsuma M., Hashimoto K., Matsukura T. 1990; Analysis of integrated human papillomavirus type 16 DNA in cervical cancers: amplification of viral sequences together with cellular flanking sequences. Journal of Virology 64:813–821
    [Google Scholar]
  22. Yoshikawa H., Kawana T., Kitagawa K., Mizuno M., Yoshikura H., Iwamoto A. 1991; Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers. Japanese Journal of Cancer Research 82:524–531
    [Google Scholar]
  23. Yousem S. A., Ohori N. P., sonmez-alpan E. 1992; Occurrence of human papillomavirus DNA in primary lung neoplasms. Cancer 69:693–697
    [Google Scholar]
  24. Zur Hausen H. 1976; Condylomata acuminata and genital cancer. Cancer Research 36:530
    [Google Scholar]
  25. Zur Hausen H. 1989; Papillomavirus in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Research 49:4677–4681
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-5-1149
Loading
/content/journal/jgv/10.1099/0022-1317-75-5-1149
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error