1887

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Volume 77, Issue 3

Long-distance movement of cherry leaf roll virus in infected tobacco plants Free

Abstract

The long-distance movement of cherry leaf roll virus (CLRV) in tobacco plants was studied using a tissue printing technique with non-isotopic RNA probes. Time-course analysis revealed that CLRV RNA accumulated in the inoculated leaf at an early stage, such as 20 h post-inoculation. The virus accumulation reached a peak at 8–10 days post-inoculation (d.p.i.) and then progressively decreased. The virus RNA signal was detected before the appearance of symptoms. The virus invaded stem vascular tissues at 3 d.p.i., moving towards the roots before moving to the upper leaves. In systemically infected leaves, the virus appeared first in the basal regions and then moved to the distal parts through the vascular system. The distribution pattern of the virus coat protein in systemically infected leaves was parallel to that observed for the virus RNA, suggesting that CLRV requires the coat protein for long-distance movement. The movement of the virus was influenced by the phyllotactic position of the leaves. The viral symptoms and the virus RNA signal in older systemically infected leaves were asymmetrically distributed, being localized in the side of the lamina closest to the inoculated leaf. Virus distribution in infected plants as well as the susceptibility of the plant to systemic infection were also influenced by the developmental stage of the inoculated leaves. Inoculation of leaves at 95% of their final size resulted in virus replication but no systemic infection. In fully mature leaves the virus did not replicate.

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© Journal of General Virology 1996
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1996-03-01
2024-04-25
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References

  1. Allison A. F., Thompson C., Ahlquist P. 1990; Regeneration of a functional RNA virus genome by recombination between deletion mutants and requirement for cowpea chlorotic mottle virus 3a and coat genes for systemic infection. Proceedings of the National Academy of Sciences, USA 87:1820–1824
     [Google Scholar]
  2. Atabekov J. G., Dorokhov Y. L. 1984; Plant virus-specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
     [Google Scholar]
  3. Atchison B. A., Francki R. I. B. 1972; The source of tobacco ringspot virus in root-tip tissue of bean plants. Physiological Plant Pathology 2:105–111
     [Google Scholar]
  4. Bennett C. W. 1940; Relation of food translocation to movement of virus of tobacco mosaic. Journal of Agricultural Research 60:361–390
     [Google Scholar]
  5. Chapman S., Hills G., Watts J., Baulcombe D. C. 1992; Mutational analysis of the coat protein gene of potato virus X: effects on virion morphology and viral pathogenicity. Virology 191:223–230
     [Google Scholar]
  6. Citovsky V., Zambryski P. 1991; How do plant virus nucleic acids move through intercellular connections?. BioEssays 13:373–379
     [Google Scholar]
  7. Dalmay T., Rubino L., Burgyan J., Russo M. 1992; Replication and movement of a coat protein mutant of Cymbidium ringspot tombusvirus. Molecular Plant–Microbe Interactions 5:379–383
     [Google Scholar]
  8. Dawson W. O., Bubrick P., Grantham G. L. 1988; Modifications of the tobacco mosaic virus coat protein gene affecting replication, movement and symptomatology. Phytopathology 78:783–789
     [Google Scholar]
  9. Deom C. M., Lapidot M., Beachy R. N. 1992; Plant virus movement proteins. Cell 69:221–224
     [Google Scholar]
  10. Ding B., Parthasarathy M. V., Niklas K., Turgeon R. 1988; A morphometric analysis of the phloem-unloading pathway in developing tobacco leaves. Planta 176:307–318
     [Google Scholar]
  11. Dolja V. V., McBride H. J., Carrington J. C. 1992; Tagging of plant potyvirus replication and movement by insertion of b-glucuronidase into the viral polyprotein. Proceedings of the National Academy of Sciences, USA 89:10208–10212
     [Google Scholar]
  12. Dolja V. V., Hadelman R., Robertson N. L., Dougherty W. G., Carrington J. C. 1994; Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants. EMBO Journal 13:1482–1491
     [Google Scholar]
  13. Flasinski S., Dzianott A., Pratt S., Bujarski J. J. 1995; Mutational analysis of the coat protein gene of brome mosaic virus: effects on replication and movement in barley and chenopodium hybridum. Molecular Plant–Microbe Interactions 8:23–31
     [Google Scholar]
  14. Gal-On A., Kaplan I., Roossinck M. J., Palukaitis P. 1994; The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 205:280–289
     [Google Scholar]
  15. Harrison B. D., Robinson D. J. 1986; Tobraviruses. In The Plant Viruses pp 339–369 Edited by van Regenmortel M. H. V., Fraenkel-Conrat H. New York: Plenum Press;
     [Google Scholar]
  16. Heaton L. A., Lee T. C., Wei N., Morris T. J. 1991; Point mutations in the turnip crinkle virus capsid protein affect the symptoms expressed by Nicotiana benthamiana . Virology 183:143–150
     [Google Scholar]
  17. Hellen C. U. T., Cooper J. I. 1987; The genome-linked protein of cherry leaf roll virus. Journal of General Virology 68:2913–2917
     [Google Scholar]
  18. Hull R. 1989; The movement of viruses in plants. Annual Review of Phytopathology 27:213–240
     [Google Scholar]
  19. Jones A. T. 1985; CLRV. CM1/AA Descriptions of Plant Viruses number 306 B
     [Google Scholar]
  20. Jones A. T., Kinninmonth A. M., Roberts I. M. 1973; Ultra-structural changes in differentiated leaf cells infected with cherry leaf roll virus. Journal of General Virology 18:61–64
     [Google Scholar]
  21. Leisner M., Turgeon R., Howell S. H. 1992; Long distance movement of cauliflower mosaic virus in infected turnip plants. Molecular Plant-Microbe Interactions 5:41–47
     [Google Scholar]
  22. Leisner R. M., Turgeon R., Howell S. H. 1993; Effects of host plant development and genetic determinants on the long-distance movement of cauliflower mosaic virus in Arabidopsis. Plant Cell 5:191–202
     [Google Scholar]
  23. Más P., Pallás V. 1995; Non-isotopic tissue-printing hybridization: a new technique to study long-distance plant virus movement. Journal of Virological Methods 52:317–326
     [Google Scholar]
  24. Más P., Sánchez-Navarro J. A., Sánchez-Pina M. A., Pallás V. 1993; Chemiluminescent and colorigenic detection of cherry leaf roll virus with digoxigenin-labeled RNA probes. Journal of Virological Methods 45:93–102
     [Google Scholar]
  25. Matthews R. E. F. 1991 Plant Virology 3rd edn New York: Academic Press;
     [Google Scholar]
  26. Maule A. J. 1991; Virus movement in infected plants. Critical Review in Plant Sciences 9:457–473
     [Google Scholar]
  27. Mircetich S. M., Samborn R. R., Ramos D. E. 1980; Natural spread, graft transmission, and possible etiology of walnut blackline disease. Phytopathology 75:962–968
     [Google Scholar]
  28. Mise K., Allison R. F., Janda M., Ahlquist P. 1993; Bromovirus movement protein genes play a crucial role in host specificity. Journal of Virology 67:2815–2823
     [Google Scholar]
  29. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Insights from amino acid sequence comparisons of movement proteins and from analogies with cellular transport systems. Archives of Virology 133:239–257
     [Google Scholar]
  30. Palukaitis P. 1987; Potato spindle tuber viroid: investigation of the long-distance, intra-plant transport route. Virology 158:239–241
     [Google Scholar]
  31. Petty I. T. D., Jackson A. O. 1990; Mutational analysis of barley stripe mosaic virus RNA β . Virology 179:712–718
     [Google Scholar]
  32. Rochon D. M., Johnston J. C., Riviere C. J. 1991; Molecular analysis of the cucumber necrosis virus genome. Canadian Journal of Plant Pathology 13:142–154
     [Google Scholar]
  33. Rowhani A., Mircetich S. M., Shepherd R. J., Cucuzza J. D. 1985; Serological detection of cherry leafroll virus in English walnut trees. Phytopathology 75:48–52
     [Google Scholar]
  34. Saito T., Yamanaka K., Okada Y. 1990; Long-distance movement and viral assembly of tobacco mosaic virus mutants. Virology 176:329–336
     [Google Scholar]
  35. Samuel G. 1934; The movement of tobacco mosaic virus within the plant. Annals of Applied Biology 21:90–111
     [Google Scholar]
  36. Schneider I. R. 1965; Introduction, translocation, and distribution of viruses in plants. Advances in Virus Research 11:163–221
     [Google Scholar]
  37. Scholthof H. B., Morris T. J., Jackson A. O. 1993; The capsid protein gene of tomato bushy stunt virus is dispensable for systemic movement and can be replaced for localized expression of foreign genes. Molecular Plant–Microbe Interactions 6:309–322
     [Google Scholar]
  38. Siegel A., Zaitlin M., Seghal O. P. 1962; The isolation of defective tobacco mosaic virus strains. Proceedings of the National Academy of Sciences, USA 48:1845–1851
     [Google Scholar]
  39. Song Y. R., Ye Z. H., Varner J. E. 1993; Tissue-printing hybridization on membrane for localization of mRNA in plant tissue. Methods in Enzymology 218:671–681
     [Google Scholar]
  40. Suzuki M., Kuwata S., Kataoka J., Masuta C., Nitta N., Takanami Y. 1991; Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113
     [Google Scholar]
  41. Takamatsu N., Ishikawa M., Meshi T., Okada Y. 1987; Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV–RNA. EM BO Journal 6:307–311
     [Google Scholar]
  42. Taliansky M. E., García-Arenal F. 1995; Role of cucumovirus capsid protein in long distance movement within the infected plant. Journal of Virology 69:916–922
     [Google Scholar]
  43. Traynor P., Young B. M., Ahlquist P. 1991; Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread. Journal of Virology 65:2807–2815
     [Google Scholar]
  44. Wellink J., van Kammen A. 1989; Cell-to-cell transport of cowpea mosaic virus requires both the 58K/48K proteins and the capsid proteins. Journal of General Virology 70:2279–2286
     [Google Scholar]
  45. White B. A., Bancroft F. C. 1982; Cytoplasmic dot hybridization. Journal of Biological Chemistry 257:8569–8572
     [Google Scholar]
  46. Wisniewski L. A., Powell Abel P., Nelson A., Beachy R. N. 1990; Local and systemic spread of tobacco mosaic virus in transgenic tobacco. Plant Cell 2:559–567
     [Google Scholar]
  47. Xiong Z., Kim K. H., Giesman-Cookmeyer D., Lommel S. A. 1993; The roles of the red clover necrotic mosaic virus capsid and cell-to-cell movement proteins in systemic infection. Virology 192:27–32
     [Google Scholar]
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Long-distance movement of cherry leaf roll virus in infected tobacco plants
J. Gen. Virol. 77, 531 (1996); https://doi.org/10.1099/0022-1317-77-3-531
/content/journal/jgv/10.1099/0022-1317-77-3-531
/content/journal/jgv/10.1099/0022-1317-77-3-531
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