1887

Journal of General Virology header logo

Volume 77, Issue 8

Feline immunodeficiency virus can infect a human cell line (MOLT-4) but establishes a state of latency in the cells Free

Abstract

Infectivity of feline immunodeficiency virus (FIV) in feline and human lymphoblastoid cell lines was examined using homogeneous populations of FIV derived from infectious molecular clones of strains TM2 and Petaluma, and two recombinant chimeric clones carrying and ORF-A from the heterologous virus. FIV from the clones with the region of the Petaluma strain was shown to infect and establish provirus in a human lymphoid cell line (MOLT-4), although the FIV-infected cells did not produce any infectious viruses. By treatment of the infected MOLT-4 cells with a phorbol ester, infectious virus was rescued. To examine which stage of the life-cycle of FIV is blocked in these cells, we analysed transcription of FIV-14 in the cells by RT-PCR. FIV-specific RNA expression could not be detected. These results strongly suggest that latency of the virus in MOLT-4 cells is due to a failure in transcription.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1996
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-8-1623
1996-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/8/JV0770081623.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-8-1623&mimeType=html&fmt=ahah

References

  1. Brunner D., Pedersen N. C. 1989; Infection of peritoneal macrophages in vitro and in vivo with feline immunodeficiency virus. Journal of Virology 63:5483–5488
     [Google Scholar]
  2. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
     [Google Scholar]
  3. Dow S. W., Poss M. L., Hoover E. A. 1990; Feline immunodeficiency vims: a neurotropic lentivirus. Journal of Acquired Immune Deficiency Syndromes 3:658–668
     [Google Scholar]
  4. Ishida T., Washizu T., Toriyabe K., Motohashi S. 1988; Detection of feline T-lymphotropic lentivirus (FTLV) infection in Japanese domestic cats. Japanese Journal of Veterinary Science 50:39–44
     [Google Scholar]
  5. Ishida T., Washizu T., Toriyabe K., Motoyoshi S., Tomoda I., Pedersen N. C. 1989; Feline immunodeficiency vims infection in cats of Japan. Journal of the American Veterinary Medical Association 194:221–225
     [Google Scholar]
  6. Kawaguchi Y., Miyazawa T., Horimoto T., Itagaki T., Fukasawa M., Takahashi E., Mikami T. 1991; Activation of feline immunodeficiency vims long terminal repeat by feline herpesvims type 1. Virology 184:449–454
     [Google Scholar]
  7. Kawaguchi Y., Maeda K., Tohya Y., Furuya T., Miyazawa T., Horimoto T., Norimine J., Kai C., Mikami T. 1992a; Replication difference in early-passage feline brain cells among feline immunodeficiency vims isolates. Archives of Virology 125:347–354
     [Google Scholar]
  8. Kawaguchi Y., Norimine J., Miyazawa T., Kai C., Mikami T. 1992b; Sequences within the feline immunodeficiency vims long terminal repeat that control transcriptional activity and respond to activation by feline herpesvims type 1. Archives of Virology 190:465–468
     [Google Scholar]
  9. Kiyomasu T., Miyazawa T., Furuya T., Shibata R., Sakai H., Sakuragi J., Fukasawa M., Maki N., Hasegawa A., Mikami T. 1991; Identification of feline immunodeficiency vims rev gene activity. Journal of Virology 65:4539–4542
     [Google Scholar]
  10. Kohmoto M., Miyazawa T., Tomonaga K., Kawaguchi Y., Mori T., Tohya Y., Kai C., Mikami T. 1994; Comparison of biological properties of feline immunodeficiency vims isolates using recombinant chimeric viruses. Journal of General Virology 75:1935–1942
     [Google Scholar]
  11. Maki N., Miyazawa T., Fukasawa M., Hasegawa A., Hayami M., Miki K., Mikami T. 1992; Molecular characterization and heterogeneity of feline immunodeficiency vims isolates. Archives of Virology 123:29–45
     [Google Scholar]
  12. Miyazawa T., Furuya T., Itagaki S., Tohya Y., Takahashi E., Mikami T. 1989a; Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency vims. Archives of Virology 108:131–135
     [Google Scholar]
  13. Miyazawa T., Furuya T., Itagaki S., Tohya Y., Nakano K., Takahashi E., Mikami T. 1989b; Preliminary comparison of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States. Archives of Virology 108:59–68
     [Google Scholar]
  14. Miyazawa T., Fukasawa M., Hasegawa A., Maki N., Ikuta K., Takahashi E., Mikami T. 1991; Molecular cloning of a novel isolate of feline immunodeficiency vims biologically and genetically different from the original U.S. isolate. Journal of Virology 65:1572–1577
     [Google Scholar]
  15. Miyazawa T., Kawaguchi Y., Kohmoto M., Sakuragi J., Adachi A., Fukasawa M., Mikami T. 1992; Production of feline immunodeficiency vims in feline and non-feline non-lymphoid cell lines by transfection of an infectious molecular clone. Journal of General Virology 73:1543–1546
     [Google Scholar]
  16. Miyoshi I., Taguchi H., Kubonishi I. 1982; Type-C virus- producing cell lines derived from adult T-cell leukemia. Gann Monographs 28:219–228
     [Google Scholar]
  17. Olmsted R. A., Barnes A. K., Yamamoto J. K., Hirsch V. M., Purcell R. H., Johnson P. R. 1989; Molecular cloning of feline immunodeficiency vims. Proceedings of the National Academy of Sciences, USA 86:2448–2452
     [Google Scholar]
  18. Pedersen N. C., Ho E. W., Brown M. L., Yamamoto J. K. 1987; Isolation of a T-Iymphotropic vims from domestic cats with an immunodeficiency-like syndrome. Science 235:790–793
     [Google Scholar]
  19. Phillips T. R., Talbott R. L., Lamont C., Muir S., Lovelace K., Elder J. H. 1990; Comparison of two host cell range variants of feline immunodeficiency vims. Journal of Virology 64:4605–4613
     [Google Scholar]
  20. Phillips T. R., Lamont C., Konings D. A. M., Shacklett B. L., Hamson C. A., Luciw P. A., Elder J. H. 1992; Identification of the Rev transactivation and Rev-responsive elements of feline immunodeficiency vims. Journal of Virology 66:5464–5471
     [Google Scholar]
  21. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
     [Google Scholar]
  22. Simon J. H. M., Schockmel G. A., Illei P., James W. 1994; A rodent cell line permissive for entry and reverse transcription of human immunodeficiency virus type X has a pre-integration block to productive infection. Journal of General Virology 75:2615–2623
     [Google Scholar]
  23. Sparger E. E., Shacklett B. L., Renshaw G. L., Barry P. A., Pedersen N. C., Elder J. H., Luciw P. A. 1992; Regulation of gene expression directed by the long terminal repeat of the feline immunodeficiency virus. Virology 187:165–177
     [Google Scholar]
  24. Theilen G. H., Kawakami T. G., Rush J. D., Munn R. J. 1969; Replication of the cat leukemia virus in cell suspension cultures. Nature 222:589–590
     [Google Scholar]
  25. Tochikura S. T., Tochikura A. T., Hays K. A., Lazo A., Bailer R. T., Blakeslee J. R., Lafrado L. J., Burman P. R., Pandey R., Olsen R. G., Mathes L. E. 1993; Fusion activity dissociated from replication ability in feline immunodeficiency virus (FIV) in human cells. Journal of Acquired Immune Deficiency Syndromes 6:1301–1310
     [Google Scholar]
  26. Tomonaga K., Norimine J., Shin Y. -S., Fukasawa M., Miyazawa T., Adachi A., Toyosaki T., Kawaguchi Y., Kai C., Mikami T. 1992; Identification of a feline immunodeficiency virus gene which is essential for cell-free infectivity. Journal of Virology 66:6181–6185
     [Google Scholar]
  27. Tomonaga K., Shin Y.-S., Fukasawa M., Miyazawa T., Adachi A., Mikami T. 1993; Feline immunodeficiency virus gene expression: analysis of the RNA splicing pattern and the monocistronirev mRNA. Journal of General Virology 74:2409–2417
     [Google Scholar]
  28. Tomonaga K., Miyazawa T., Kawaguchi Y., Kohmoto M., Inoshima Y., Mikami T. 1994; Comparison of the Rev transactivation of feline immunodeficiency virus in feline and non-feline cell lines. Japanese Journal of Veterinary Medical Science 56:199–201
     [Google Scholar]
  29. Willey R. L., Smith D. H., Lasky L. A., Theodora T. S., Earl P. L., Moss B., Capon D. J., Martin M. A. 1988; In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. Journal of Virology 62:139–147
     [Google Scholar]
  30. Yamamoto J. K., Sparger E. E., Ho E. W., Andersen P. R., O’Connor T. P., Mandell C. P., Lowenstine L., Munn R., Pedersen N. C. 1988; Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. American Journal of Veterinary Research 49:1246–1258
     [Google Scholar]
  31. Yamamoto J. K., Hansen H., Ho E. W., Morishita T. Y., Okuda T., Sawa T. R., Nakamura R. M., Pedersen N. C. 1989; Epidemiologic and clinical aspects of feline immunodeficiency virus infection in cats from the continental United States and Canada and possible mode of transmission. Journal of the American Veterinary Medical Association 194:213–220
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-8-1623
Loading
Feline immunodeficiency virus can infect a human cell line (MOLT-4) but establishes a state of latency in the cells
J. Gen. Virol. 77, 1623 (1996); https://doi.org/10.1099/0022-1317-77-8-1623
/content/journal/jgv/10.1099/0022-1317-77-8-1623
/content/journal/jgv/10.1099/0022-1317-77-8-1623
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error