1887

Abstract

T cell proliferative responses against human papillomavirus type 16 (HPV-16) E7 protein were studied in relation to HPV status over time in 51 women originally diagnosed with abnormal cervical cytology and participating in a follow-up study. HPV-16-positive patients were grouped as having either a persistent, a cleared or a fluctuating HPV-16 infection as determined by PCR in consecutive cervical smears up until the moment of testing. Positive proliferative responses against HPV-16 E7 were found in 15/26 patients with a persistent, cleared or fluctuating HPV-16 infection (57.7%). In contrast, 0/15 patients who had been typed HPV-negative during follow-up showed positive responses ( = 0.0005). Further analysis showed positive responses to be more frequent in patients with persistent HPV-16 infections and stable or progressing cervical lesions (8/9 patients reactive, 88.9%) as compared to patients with cleared or fluctuating HPV-16 infections and stable or regressing cervical lesions (7/17, 41.1%, = 0.04). The relatively strong T cell proliferative responses against HPV-16 E7 observed in patients with a persistent HPV-16 infection and progressive cervical lesions indicate that the effectivity of such responses cannot be predicted and apparently depends on additional factors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2183
1996-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/9/JV0770092183.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-9-2183&mimeType=html&fmt=ahah

References

  1. Altmann A., Jochmus-Kudielka J., Frank R., Gausepohl H., Moebius U., Gissmann L., Meuer S. C. 1992; Definition of immunogenic determinants of the human papillomavirus type 16 nucleoprotein E7. European Journal of Cancer 28:326–333
    [Google Scholar]
  2. Apple R. J., Erlich H. A., Klitz W., Manos M. M., Becker T. M., Wheeler C. M. 1994; HLA-DR-DQ associations with cervical carcinoma show papillomavirus-type specificity. Nature Genetics 6:157–162
    [Google Scholar]
  3. Bal V., Mclndoe A., Denton G., Hudson D., Lombardi G., Lamb J., Lechler R. 1990; Antigen presentation by keratinocytes induces tolerance in human T cells. European Journal of Immunology 20:1893–1897
    [Google Scholar]
  4. Biron C. 1994; Cytokines in the generation of immune responses to and resolution of virus infection. Current Opinions in Immunology 6:530–538
    [Google Scholar]
  5. Chen L., Kinney Thomas E., Hu S.-L., Hellstrom I., Hellsrom K. E. 1991; Human papillomavirus type 16 nucleoprotein E7 is a tumor rejection antigen. Proceedings of the National Academy of Sciences, USA 88:110–114
    [Google Scholar]
  6. Clerici M., Shearer G. M. 1993; A Th1/Th2 switch is a critical step in the etiology of HIV infection. Immunology Today 14:107–111
    [Google Scholar]
  7. Comerford S. A., McCance D. J., Dougan G., Tite J. P. 1991; Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16. Journal of Virology 65:4681–4690
    [Google Scholar]
  8. Cromme F. V., Meijer C. J. L. M., Snijders P. J. F., Uyterlinde A., Kenemans P., Helmerhorst Th., Stern P. L., van den Brule A. J. C., Walboomers J. M. M. 1993; Analysis of MHC class I and II expression in relation to presence of HPV genotype in premalignant and malignant cervical lesions. British Journal of Cancer 68:1372–1380
    [Google Scholar]
  9. Cubie H. A., Norval M. 1988; Humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. Journal of Medical Virology 24:85–95
    [Google Scholar]
  10. Cubie H. A., Norval M., Crawford L., Banks L., Crook T. 1989; Lymphoproliferative response to fusion proteins of human papillomaviruses in patients with cervical intraepithelial neoplasia. Epidemiology and Infection 103:625–632
    [Google Scholar]
  11. De Roda Husman A. M., Walboomers J. M. M., Meijer C. J. L. M., Risse E. K. J., Schipper M. E. I., Helmerhorst Th. J. M., Bleker O. P., van den Brule A. J. C., Snijders P. J. F. 1994; Analysis of cytomorphologically abnormal cervical scrapes for the presence of 27 mucosotropic human papillomavirus genotypes using polymerase chain reaction. International Journal of Cancer 56:802–806
    [Google Scholar]
  12. Duggan-Keen M., Keating P. J., Cromme F. V., Walboomers J. M. M., Stern P. L. 1994; Alterations in major histocompatibility complex expression in cervical cancer: possible consequences for immunotherapy. Papillomaviruses Report 5:3–9
    [Google Scholar]
  13. Feltkamp M. C. W., Smits H. L., Vierboom M. P. M., Minnaar R. P., de Jongh B. M., Drijfhout J. W., ter Schegget J., Melief C. J. M., Kast M. W. 1993; Vaccination with cytotoxic T lymphocyte epitope-containing peptide protects against a tumor induced by human papillomavirus type 16-transformed cells. European Journal of Immunology 23:2242–2249
    [Google Scholar]
  14. Glew S. S., Duggan-Keen M., Cabrera T., Stern P. L. 1992; HLAclass II antigen expression in human papillomavirus-associated cervical cancer. Cancer Research 52:4009–4006
    [Google Scholar]
  15. Goodman R. E., Nestle F., Yathi M. N., Green J. M., Thompson C. B., Nickoloff B. J., Turka L. A. 1994; Keratinocyte-derived T cell costimulation induces preferential production of IL-2 and IL-4 but not IFN-y. Journal of Immunology 152:5189–5198
    [Google Scholar]
  16. Halpert R., Fruchter R. G., Sedlis A., Butt K., Boyce J. G., Sillman H. 1986; Human papillomavirus and lower genital neoplasia in renal transplant patients. Obstetrics and Gynecology 68:251–258
    [Google Scholar]
  17. Kadish A. S., Romney S. L., Ledwidge R., Tindle R., Fernando J. P., Zee S. Y., van Ranst M. A., Burk R. D. 1994; Cell-mediated immune responses to E7 peptides of human papillomavirus (HPV) type 16 are dependent on the HPV type infecting the cervix whereas serological reactivity is not type-specific. Journal of General Virology 75:2277–2284
    [Google Scholar]
  18. Laga M., Icenogle J. P., Marsella R., Manoka A. T., Nzila N., Ryder R. W., Vermund S. H., Heyward W. L., Nelson A., Reeve W. C. 1992; Genital papillomavirus infection and cervical dysplasia-opportunistic complications of HIV infection. International Journal of Cancer 48:682–688
    [Google Scholar]
  19. McLean C. S., Sterling J. S., Mowat J., Nash A. A., Stanley M. A. 1993; Delayed-type hypersensitivity response to the human papillomavirus type 16 E 7 protein in a mouse model. Journal of General Virology 74:239–245
    [Google Scholar]
  20. Manca F., Li Pira G., Fenoglio D., Valle M. T., Kunkl A., Ferraris A., Lancia F., Saverino D., Martara L., Balderas R., Arp J., Dekaban G., Dalgleish A. G., Lozzi L., Theofilopoulos A. N. 1995; Recognition of human T-leukemia virus (HTLV-1) envelope by human CD4+ T-cell lines from HTLV-1 seronegative individuals: specificity and clonal heterogeneity. Blood 84:1547–1554
    [Google Scholar]
  21. Muller G., Saloga J., Germann T., Bellinghausen I., Mohamadzadeh M., Knop J., Enk H. 1994; Identification and induction of human keratinocyte-derived IL-12. Journal of Clinical Investigations 94:1799–1805
    [Google Scholar]
  22. Müller M., Viscidi R. P., Sun Y., Guerrero E., Hill P. M., Shah F., Bosch F. X., Munoz N., Gissmann L., Shah K. 1992; Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16 associated invasive cervical cancer. Virology 187:508–514
    [Google Scholar]
  23. Remmink A. J., Walboomers J. M. M., Helmerhorst Th. J. M., Voorhorst F. J., Rozendaal L., Risse E. K. J., Meijer C. J. L. M., Kenemans P. 1995; The presence of persistent high-risk HPV genotypes in dysplastic cervical lesions is associated with progressive disease: natural history up to 36 months. International Journal of Cancer 61:306–311
    [Google Scholar]
  24. Schäfer A., Friedmann W., Mielke M., Schwartlander B., Koch M. A. 1991; The increased frequency of cervical dysplasia-neoplasia in women infected with the human immunodeficiency virus is related to the degree of immunosuppression. American Journal of Obstetrics and Gynecology 164:593–599
    [Google Scholar]
  25. Selvakumar R., Borenstein L. A., Lin Y.-L., Ahmed R., Wettstein F. O. 1994; T-cell response to cottontail rabbit papillomavirus structural proteins in infected rabbits. Journal of Virology 68:4043–4048
    [Google Scholar]
  26. Shepherd P. S., Tran T. T. T., Rowe A. J., Cridland J. C., Comerford S. A., Chapman M. G., Rayfield L. S. 1992; T cell responses to the human papillomavirus type 16 E7 protein in mice of different haplotypes. Journal of General Virology 73:1269–1274
    [Google Scholar]
  27. Smith D. B., Johnson K. S. 1988; Single-step purification of polypeptide expressed in E. coli as fusions with glutathione-S-transferase. Gene 67:31–40
    [Google Scholar]
  28. Steele J. C., Stankovic T., Gallimore P. H. 1993; Production and characterization of human proliferative T-cell clones specific for human papillomavirus type-1 E4 protein. Journal of Virology 67:2799–2806
    [Google Scholar]
  29. Strang G., Hickling J. K., Mclndoe G. A. J., Howland K., Wilkinson D., Ikeda H., Rothbard J. B. 1990; Human T cell responses to human papillomavirus type 16 LI and E6 synthetic peptides: identification of T cell determinants, HLA-DR restriction, and virus type specificity. Journal of General Virology 71:423–431
    [Google Scholar]
  30. Suchankova A., Krcmar M., Krchnak V., Hamsikova E., Kanka J., Vagner J., Vonka V. 1992; Range of HPV-16 E7 antibodies in cervical cancer patients and healthy subjects. International Journal of Cancer 51:837–838
    [Google Scholar]
  31. Tindle R. W., Fernando G. J. P., Sterling J. C., Frazer I. H. 1991; A ‘public’ T-helper epitope of the E7 transforming protein of human papillomavirus 16 provides cognate help for several E7 B-cell epitopes from cervical cancer-associated human papillomavirus genotypes. Proceedings of the National Academy of Sciences, USA 88:5887–5891
    [Google Scholar]
  32. Van den Brule A. J. C., Meijer C. J. L. M., Bakels V., Kenemans P., Walboomers J. M. M. 1990; Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction. Journal of Clinical Microbiology 28:2739–2743
    [Google Scholar]
  33. Viscidi R. P., Sun Y., Tsuzaki B., Bosch F. X., Munoz N., Shah K. V. 1993; Serologic response in human papillomavirus-associated invasive cervical cancer. International Journal of Cancer 55:780–784
    [Google Scholar]
  34. Zur Hausen H. 1994; Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types. In Human Pathogenic Papillomaviruses pp 131–156 Edited by Zur Hausen H. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2183
Loading
/content/journal/jgv/10.1099/0022-1317-77-9-2183
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error