1887

Abstract

Little sequence information is available for human immunodeficiency virus type 1 (HIV-1) genes of African origin. Here we describe 37 new complete genes of 18 AIDS patients from Uganda and showthat hasa high genetic variability. proviral DNA sequences of peripheral blood cells were determined by direct sequencing of PCR products. Only 52% of the deduced Vif amino acids were absolutely conserved; when Vif sequences previously analysed were considered, only 32% of the Vif consensus sequence comprised conserved and as such possibly functionally important motifs. The high inter-individual variability was in contrast to a very low intra-individual variability. One patient carried a gene with a stable C-terminal deletion, but N-terminal truncations were not found in patients’ predominant sequences. The genes analysed comprised subtypes A, D and an A/D mosaic. Phylogenetic analyses additionally showed that HIV-1 in Uganda has spread across the boundaries of ethnic groups.

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1997-02-01
2024-04-16
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References

  1. Bachmann M. H., Delwart E. L., Shpaer E. G., Lingenfelter P., Singal R., Mullins J. I. The WHO Network for HIV Isolation andCharacterization 1994; Rapid genetic characterization of HIV type 1 strains from four World Health Organization-sponsored vaccine evaluation sites using a heteroduplex mobility assay. AIDS Research and Human Retroviruses 10:1345–1353
    [Google Scholar]
  2. Bruce C., Clegg C., Featherstone A., Smith J., Biryahawaho B., Downing R., Oram J. 1994; Presence of multiple genetic subtypes of human immunodeficiency virus type 1 proviruses in Uganda. AIDS Research and Human Retroviruses 10:1543–1550
    [Google Scholar]
  3. Buonaguro L., Del Gaudio E., Monaco M., Greco D., Corti P., Beth-Giraldo E., Buonaguro F. M., Giraldo G. the Italian-Ugandan Cooperation AIDS Program 1995; Heteroduplex mobility assay and phylogenetic analysis of V3 region sequences of human immunodeficiency virus type 1 isolates from Gulu, Northern Uganda. Journal of Virology 69:7971–7981
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution 17:368–376
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Goncalves J., Shi B., Yang X., Gabuzda D. 1995; Biological activity of human immunodeficiency virus type 1 vif requires membrane targetingby C-terminal basic domains. Journal of Virology 69:7196–7204
    [Google Scholar]
  7. Karczewski M., Strebel K. 1996; Cytoskeleton association and virion incorporation of the human immunodeficiency virus type 1 vif protein. Journal of Virology 70:494–507
    [Google Scholar]
  8. Kunanusont C., Foy H. M., Kreiss J. K., Rerks-Ngarm S., Phanuphak P., Raktham S., Pau C. P., Young N. L. 1995; HIV-1 subtypes and male-to-female transmission in Thailand. Lancet 345:1078–1083
    [Google Scholar]
  9. Kishino H., Hasegawa M. 1989; Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. Journal of Molecular Evolution 29:170–179
    [Google Scholar]
  10. Ma X. Y., Pavel S., Chao W., Volsky D. J. 1994; Cysteine residues in the vif protein of human immunodeficiency virus type 1 are essential for viral infectivity. Journal of Virology 68:1714–1720
    [Google Scholar]
  11. Michael N. L., Chang G., D’Arcy L. A., Ehrenberg P. K., Mariani R., Busch M. P., Birx D. L., Schwartz D. H. 1995; Defective accessory genes in a human immunodeficiency virus type 1-infected long-term survivor lacking recoverable virus. Journal of Virology 69:4228–4236
    [Google Scholar]
  12. Mulder J., McKinney N., Christopherson C., Sninsky J., Greenfield L., Kwok S. 1994; Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma : application to acute retroviral infection. Journal of Clinical Microbiology 32:292–300
    [Google Scholar]
  13. Myers G., Korber B., Hahn B. H., Jeang K. T., Mellors J. W., McCutchan F. E., Henderson L. E., Pavlakis G. N.editors 1995 Human Retroviruses and AIDS 1995 Los Alamos, N. Mex: Los Alamos National Laboratory;
    [Google Scholar]
  14. Ochsenbauer C., Bosch V., Oelze I., Wieland U. 1996; Unimpaired function of a naturally occurring C terminally truncated vif gene product of human immunodeficiency virus type 1. Journal of General Virology 77:1389–1395
    [Google Scholar]
  15. Olivares I., Menendez-Arias L., Rodriguez-Bernabe A., Martin M. J., Dopazo J., Lopez-Galindez C. 1995; Sequence analysis ofHIV-1 vif gene in Spanish isolates. Virus Genes 9:283–288
    [Google Scholar]
  16. Robertson D. L., Hahn B. H., Sharp P. M. 1995a; Recombination in AIDS viruses. Journal of Molecular Evolution 40:249–259
    [Google Scholar]
  17. Robertson D. L., Sharp P. M., McCutchan F. E., Hahn B. H. 1995b; Recombination in HIV-1. Nature 374:124–126
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  19. Salminen M. O., Carr J. K., Burke D. S., McCutchan F. E. 1995; Genotyping of HIV-1. In Human Retroviruses and AIDS 1995 pp. III-30–III-34 Myers G., Korber B., Hahn B. H., Jeang K. T., Mellors J. W., McCutchan F. E., Henderson L. E., Pavlakis G. N., Los Alamos N. Edited by Mex: Los Alamos National Laboratory;
    [Google Scholar]
  20. Schwander S., Rüsch-Gerdes S., Mateega A., Lutalo T., Tugume S., Aisu T., Kityo C., Rubaramira R., Mugerwa R., Okwera R., Moser R., Ochen K., Mugyenyi P., M’Bonye B., Dietrich M. 1995; A pilot study of antituberculous combinations comparing rifabutin with ri- fampicin in the treatment of HIV-associated tuberculosis. A single blinded randomized evaluation in Ugandan patients with HIV-1 infection and pulmonary tuberculosis. Tubercle and Lung Disease 76:210–218
    [Google Scholar]
  21. Sova P., van Ranst M., Gupta P., Balachandran R., Chao W., Itescu S., McKinley G., Volsky D. J. 1995; Conservation of an intact human immunodeficiency virus type 1 vif gene in vitro and in vivo. Journal of Virology 69:2557–2564
    [Google Scholar]
  22. Subbramanian R., Cohen E. A. 1994; Molecular biology of the human immunodeficiency virus accessory proteins. Journal of Virology 68:6831–6835
    [Google Scholar]
  23. Wieland U., Hartmann J., Suhr H., Salzberger B., Eggers H. J., Kühn J. E. 1994; In vivo genetic variability of the HIV-1 vif gene. Virology 203:43–51
    [Google Scholar]
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