1887

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Volume 78, Issue 8

Analysis of the biochemical properties of, and complex formation between, glycoproteins H and L of the γ herpesvirus bovine herpesvirus-4. Free

Abstract

Genes encoding glycoprotein gH and gL homo- logues were localized in the genome of the gamma- herpesvirus bovine herpesvirus-4 (BHV-4). Both genes were sequenced and glutathione S-trans- ferase fusion proteins were produced and used to immunize rabbits against the translation products of the two genes. The anti-gH serum recognized a protein with an apparent molecular mass (MM) of 110 kDa both in infected cells and in virions. This protein was sensitive to endo- - -acetylglucos- aminase-H (endoH) and endoglycosidase F--gly- cosidase F (endoF-PNGaseF) digestion. A protein with the same relative mobility was immunopre- cipitated from infected cells radiolabelled with [H]glucosamine which confirmed that this product (gp110), now designated BHV-4 gH, was glycosylated. Western blotting with the anti-gL serum detected in infected cells a product with an apparent MM ranging from 31–35 kDa and diffusely migrating proteinspeciesrangingfrom45–65 kDa.Tunica- mycin, monensin, endoH or endoF-PNGaseF treatments showed that both the 31–35 kDa and the 45–65 kDa proteins were glycosylated, gp31–35 being a precursor of the 45–65 kDa glycoprotein species. In radioimmunoprecipitation assays, the anti-gL serum immunoprecipitated from infected cells two glycosylated proteins with apparent MMs of 31–35 kDa (gp31–35) and 45–55 kDa (gp45–55). However a third glycoprotein, gp110, was also immunoprecipitated together with gp31–35 and gp45–55. gp110 and gp45–55 were subsequently confirmed to be virion glycoproteins corresponding to mature forms of BHV-4 gH and gL respectively. In addition, the present study clearly demonstrated complex formation between BHV-4 gH and gL both in virions and in infected cells.

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© Society for General Microbiology 1997
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1997-08-01
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Analysis of the biochemical properties of, and complex formation between, glycoproteins H and L of the γ2 herpesvirus bovine herpesvirus-4.
J. Gen. Virol. 78, 2015 (1997); https://doi.org/10.1099/0022-1317-78-8-2015
/content/journal/jgv/10.1099/0022-1317-78-8-2015
/content/journal/jgv/10.1099/0022-1317-78-8-2015
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