1887

Abstract

Infection with hepatitis G virus (HGV) or GB virus-C (GBV-C) is widely distributed in human populations. Viruses related to GBV-C/HGV have been recovered from several New World primate species, including tamarins, owl monkeys and marmosets. To understand more about the relationship between GB viruses and their hosts, we used primers from the 5′ non-coding (5NC), non-structural 3 (NS3) and NS5 regions in nested polymerase chain reactions to screen for related viruses infecting non-captive chimpanzees ( and subspecies). Sequences from the 5NCR and NS5 regions were amplified from samples taken from 3 of 39 chimpanzees, and from one chimpanzee in the NS3 region. Sequence comparisons of each region revealed that the GB virus infecting chimpanzees was distinct from both GBV-C/HGV and from any of the known GBV-A sequences, but was more closely related to human viruses. GB viruses recovered from different chimpanzees were more diverse than variants of GBV-C/HGV found in humans, with 25% sequence divergence in the 5′NCR and 20% (9·5% amino acid) sequence divergence in NS5 between variants recovered from the and subspecies, compared with 7·4% and 10·4% (1·9% amino acid) divergence amongst GBV-C/HGV variants infecting humans. Finding GBV-C/HGV-related viruses in an Old World monkey species suggests that GB-like viruses may be widely distributed in simians, and suggests a close evolutionary relationship with their natural hosts.

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1998-08-01
2024-04-24
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References

  1. Bukh J., Apgar C. L. 1997; Five new or recently discovered (GBV-A) virus species are indigenous to new world monkeys and may constitute a separate genus of the Flaviviridae. Virology 229:429–436
    [Google Scholar]
  2. Choo Q. L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., MedinaSelby R., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, USA 88:2451–2455
    [Google Scholar]
  3. Erker J. C., Desai S. M., Leary T. P., Chalmers M. L., Montes C. C., Mushahwar I. K. 1998; Genomic analysis of two GB virus A variants isolated from captive monkeys. Journal of General Virology 79:41–45
    [Google Scholar]
  4. Jarvis L. M., Watson H. G., McOmish F., Peutherer J. F., Ludlam C. A., Simmonds P. 1994; Frequent reinfection and reactivation of hepatitis C virus genotypes in multitransfused hemophiliacs. Journal of Infectious Diseases 170:1018–1022
    [Google Scholar]
  5. Jones S., Martin R., Pilbeam D. 1992 Human Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  6. Kumar S., Tamura K., Nei M. 1993; MEGA: Molecular Evolutionary Genetics Analysis. version 1.0 Pennsylvania: Pennsylvania State University;
    [Google Scholar]
  7. Leary T. P., Desai S. M., Yamaguchi J., Chalmers M. L., Schlauder G. G., Dawson G. J., Mushahwar I. K. 1996a; Species-specific variants of GB virus A in captive monkeys. Journal of Virology 70:9028–9030
    [Google Scholar]
  8. Leary T. P., Muerhoff A. S., Simons J. N., Pilot-Matias T. J., Erker J. C., Chalmers M. L., Schlauder G. G., Dawson G. J., Desai S. M., Mushahwar I. K. 1996b; Sequence and genomic organization of GBV-C: a novel member of the flaviviridae associated with human non-A-E hepatitis. Journal of Medical Virology 48:60–67
    [Google Scholar]
  9. Leary T. P., Desai S. M., Erker J. C., Mushahwar I. K. 1997; The sequence and genomic organization of a GB virus A variant isolated from captive tamarins. Journal of General Virology 78:2307–2313
    [Google Scholar]
  10. Linnen J., Wages J., Zhangkeck Z. Y., Fry K. E., Krawczynski K. Z., Alter H., Koonin E., Gallagher M., Alter M., Hadziyannis S., Karayiannis P., Fung K., Nakatsuji Y., Shih J. W. K., Young L., Piatak M., Hoover C., Fernandez J., Chen S., Zou J. C., Morris T., Hyams K. C., Ismay S., Lifson J. D., Hess G., Foung S. K. H., Thomas H., Bradley D., Margolis H., Kim J. P. 1996; Molecular cloning and disease association of hepatitis G virus : a transfusion- transmissible agent. Science 271:505–508
    [Google Scholar]
  11. Nakao H., Okamoto H., Fukuda M., Tsuda F., Mitsui T., Masuko K., Lizuka H., Miyakawa Y., Mayumi M. 1997; Mutation rate of GB virus C hepatitis G virus over the entire genome and in subgenomic regions. Virology 233:43–50
    [Google Scholar]
  12. Nishizawa T., Okamoto H., Konishi K., Yoshizawa H., Miyakawa Y., Mayumi M. 1997; A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown aetiology. Biochemical and Biophysical Research Communications 241:92–97
    [Google Scholar]
  13. Schlauder G. G., Dawson G. J., Simons J. N., Pilot-Matias T. J., Guiterrez R. A., Heynen C. A., Knigge M. F., Kurpieqski G. S., Buijk S. L., Leary T. P., Muerhoff A. S., Desai S. M., Mushahwar I. K. 1995; Molecular and serologic analysis in the transmission of the GB hepatitis agents. Journal of Medical Virology 46:81–90
    [Google Scholar]
  14. Simons J. N., Pilot-Matias T. J., Leary T. P., Dawson G. J., Desai S. M., Schlauder G. G., Muerhoff A. S., Erker J. C., Buijk S. L., Chalmers M. L., Vansant C. L., Mushahwar I. K. 1995; Identification of two flavivirus-like genomes in the GB hepatitis agent. Proceedings of the National Academy of Sciences, USA 92:3401–3405
    [Google Scholar]
  15. Simons J. N., Desai S. M., Schultz D. E., Lemon S. M., Mushahwar I. K. 1996; Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization. Journal of Virology 70:6126–6135
    [Google Scholar]
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