1887

Abstract

Measles virus (MV)-induced immune suppression during acute measles often leads to secondary viral, bacterial and parasitic infections which severely complicate the course of disease. Previously, we have shown that cotton rats are a good animal model to study MV-induced immune suppression, where proliferation inhibition after stimulation of cotton rat spleen cells is induced by the viral glycoproteins (fusion and haemagglutinin proteins). We have now tested a variety of putative mechanisms of MV-induced immune suppression in this animal model. Proliferation inhibition is not due to fusion mediated by the MV glycoproteins and subsequent lysis of cells. Other putative mechanisms like classical anergy (unresponsiveness towards IL-2) or apoptosis do not seem to play a role in MV-induced immune suppression. In contrast, it was shown that spleen cells from infected animals preferentially accumulate in the G/G phase and progress more slowly through the cell cycle after mitogen stimulation in comparison to cells from non-infected animals. These data indicate a retardation of the cell cycle which is correlated with proliferation inhibition and might have severe consequences in mounting an effective immune response.

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1999-08-01
2024-04-18
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References

  1. Auwaerter P. G., Kaneshima H., McCune J. M., Wiegand G., Griffin D. E. 1996; Measles virus infection of thymic epithelium in the SCID-hu mouse leads to thymocyte apoptosis. Journal of Virology 70:3734–3740
    [Google Scholar]
  2. Esolen L. M., Ward B. J., Moench T. R., Griffin D. E. 1993; Infection of monocytes during measles. Journal of Infectious Diseases 168:47–52
    [Google Scholar]
  3. Esolen M. E., Park S. W., Hardwick J. M., Griffin D. E. 1995; Apoptosis as a cause of death in measles virus-infected cells. Journal of Virology 69:3955–3958
    [Google Scholar]
  4. Fugier-Vivier I., Servet-Delphrat C., Rivallier P., Rissoan M. C., Liu Y. J., Rabourdin-Combe C. 1997; Measles virus suppresses cell-mediated immunity by interfering with the survival and functions of dendritic and T cells. Journal of Experimental Medicine 186:813–823
    [Google Scholar]
  5. Griffin D. E. 1995; Immune responses during measles virus infection. In Measles virus pp 117–134 Edited by Billeter M., ter Meulen V. Berlin: Springer;
    [Google Scholar]
  6. Griffin D. E., Johnson R. T., Tamashiro V. G., Moench T. R., Jauregi E., Lindo de Sonariano I., Vaisberg A. 1987; In vitro studies of the role of monocytes in the immune suppression associated with natural measles virus infection. Clinical Immunology and Immunopathology 45:375–383
    [Google Scholar]
  7. Karp C. L., Wysocka M., Wahl L. M., Ahearn J. M., Cuomo P. J., Sherry B., Trinchieri G., Griffin D. E. 1996; Mechanism of suppression of cell-mediated immunity by measles virus. Science 273:228–231
    [Google Scholar]
  8. Leopardi R., Ilonen J., Mattila L., Salmi A. 1993; Effect of measles virus infection on MHC class II expression and antigen presentation in human monocytes. Cellular Immunology 147:388–396
    [Google Scholar]
  9. Lyons A. B., Parish C. R. 1994; Determination of lymphocyte division by flow cytometry. Journal of Immunological Methods 171:131–137
    [Google Scholar]
  10. McChesney M. B., Kehrl J. H., Valsamakis A., Fauci A. S., Oldstone M. B. A. 1987; Measles virus infection of B lymphocytes permits cellular activation but blocks progression through the cell cycle. Journal of Virology 61:3441–3447
    [Google Scholar]
  11. McChesney M. B., Altmann A., Oldstone M. B. A. 1988; Suppression of T lymphocyte function by measles virus is due to cell cycle arrest in G1. Journal of Virology 140:1269–1273
    [Google Scholar]
  12. Nakayama T., Mori T., Yamaguchi S., Sonoda S., Asamura S., Yamashity R., Takeuchi Y., Urano T. 1995; Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability. Virus Research 35:1–16
    [Google Scholar]
  13. Niewiesk S., Eisenhuth I., Fooks A., Clegg J. C. S., Schnorr J.-J., Schneider-Schaulies S., ter Meulen V. 1997a; Measles virus-induced immune suppression in the cotton rat ( Sigmodon hispidus ) model depends on viral glycoproteins. Journal of Virology 71:7214–7219
    [Google Scholar]
  14. Niewiesk S., Schneider-Schaulies J., Ohnimus H., Jassoy C., Schneider-Schaulies S., Diamond L., Logan J. S., ter Meulen V. 1997b; CD46 expression does not overcome the intracellular block of measles virus replication in transgenic rats. Journal of Virology 71:7969–7973
    [Google Scholar]
  15. Nussbaum O., Broder C. C., Moss B., Stern L. B., Rozenblatt S., Berger E. A. 1995; Functional and structural interactions between measles virus hemagglutinin and CD46. Journal of Virology 69:3341–3349
    [Google Scholar]
  16. Ohnimus H., Heinkelein M., Jassoy C. 1997; Apoptotic cell death upon contact of CD4+ T lymphocytes with HIV glycoprotein-expressing cells is mediated by caspases but bypasses CD95 (Fas/APO-1) and TNF receptor 1. Journal of Immunology 159:5246–5252
    [Google Scholar]
  17. Ravanel K., Castelle C., Defrance T., Wild T. F., Charron D., Lotteau V., Rabourdin-Combe C. 1997; Measles virus nucleocapsid protein binds to FcγRII and inhibits human B cell antibody production. Journal of Experimental Medicine 186:269–278
    [Google Scholar]
  18. Rodriguez I., Matsuura K., Khatib K., Reed J. C., Nagata S., Vassalli P. 1996; A bcl-2 transgene expressed in hepatocytes protects mice from fulminant liver destruction but not from rapid death induced by anti-Fas antibody injection. Journal of Experimental Medicine 183:1031–1036
    [Google Scholar]
  19. Sanchez-Lanier M., Guerlin P., McLaren L. C., Bankhurst A. D. 1988; Measles-induced suppression of lymphocyte proliferation. Cellular Immunology 116:367–381
    [Google Scholar]
  20. Sarin A., Wu M. L., Henkart P. A. 1996; Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli. Journal of Experimental Medicine 184:2445–2450
    [Google Scholar]
  21. Schlender J., Schnorr J. J., Cattomen T., Cattaneo R., Billeter M. A., ter Meulen V., Schneider-Schaulies S. 1996; Surface interaction of measles virus glycoproteins is necessary and sufficient for the induction of proliferative inhibition of human peripheral blood mononuclear cells. Proceedings of the National Academy of Sciences USA 93:13194–13199
    [Google Scholar]
  22. Schnorr J.-J., Seufert M., Schlender J., Borst J., Johnson I. C. D., ter Meulen V., Schneider-Schaulies S. 1997; Cell cycle arrest rather than apoptosis is associated with measles virus contact-mediated immunosuppression in vitro. Journal of General Virology 78:3217–3226
    [Google Scholar]
  23. Sum X., Burns J. B., Howell J. M., Fujinami R. S. 1998; Suppression of antigen-specific T cell proliferation by measles virus infection: role of a soluble factor in suppression. Virology 246:24–33
    [Google Scholar]
  24. Taylor I. W., Milthorpe B. K. 1980; An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. Journal of Histochemistry and Cytochemistry 28:1224–1232
    [Google Scholar]
  25. Tishon A., Manchester M., Scheiflinger F., Oldstone M. B. A. 1996; A model of measles virus-induced immunosuppression: enhanced susceptibility of neonatal human peripheral blood lymphocytes. Nature Medicine 2:1250–1254
    [Google Scholar]
  26. von Pirquet C. 1908; Das Verhalten der kutanen Tuberkulin-Reaktion während der Masern. Deutsche Medizinische Wochenschrift 34:1297–1300
    [Google Scholar]
  27. Walker B. D., Flexner C., Paradis T. J., Fuller T. C., Hirsch M. S., Schooley R. T., Moss B. 1988; HIV-1 reverse transcriptase is a target for cytotoxic T lymphocytes in infected individuals. Science 240:64–66
    [Google Scholar]
  28. Ward B. J., Griffin D. E. 1991; Changes in cytokine production after measles virus vaccination: predominant production of IL-4 suggests induction of Th2 response. Clinical Immunology and Immunopathology 67:171–177
    [Google Scholar]
  29. Wild T. F., Bernard A., Spehner D., Drillien R. 1992; Construction of vaccinia virus recombinants expressing several measles virus proteins and analysis of their efficacy in vaccination of mice. Journal of General Virology 73:359–367
    [Google Scholar]
  30. Yanagi Y., Cubitt B., Oldstone M. B. A. 1992; Measles virus inhibits mitogen-induced T cell proliferation but does not directly perturb the T cell activation process inside the cell. Virology 187:280–289
    [Google Scholar]
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