1887

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Volume 83, Issue 3

Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus Free

Abstract

To identify new T-cell epitopes of classical swine fever virus (CSFV), 573 overlapping, synthetic pentadecapeptides spanning 82% of the CSFV (strain Glentorf) genome sequence were synthesized and screened. In proliferation assays, 26 peptides distributed throughout the CSFV viral protein sequences were able to induce specific T-cell responses in PBMCs from a CSFV-Glentorf-infected d/d haplotype pig. Of these 26 peptides, 18 were also recognized by PBMCs from a CSFV-Alfort/187-infected d/d haplotype pig. In further experiments, it could be shown that peptide 290 (KHKVRNEVMVHWFDD), which corresponds to amino acid residues 1446–1460 of the CSFV non-structural protein NS2–3 could induce interferon-γ secretion after secondary restimulation. The major histocompatibility complex (MHC) restriction for stimulation of T-cells by this pentadecapeptide was identified as being mainly MHC class II and partially MHC class I. In cytolytic assays, CSFV-specific cytotoxic T-lymphocytes (CTLs) were able to lyse peptide 290-loaded target cells. These findings indicate the existence of a CSFV-specific helper T-cell epitope and a CTL epitope in this peptide.

  • Received:
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© Microbiology Society
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2002-03-01
2024-04-24
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Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus
J. Gen. Virol. 83, 551 (2002); https://doi.org/10.1099/0022-1317-83-3-551
/content/journal/jgv/10.1099/0022-1317-83-3-551
/content/journal/jgv/10.1099/0022-1317-83-3-551
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