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Volume 83, Issue 5

The products of the UL10 (gM) and the UL49.5 genes of Marek’s disease virus serotype 1 are essential for virus growth in cultured cells Free

Abstract

The role of the products of the UL10 and the UL49.5 homologous genes of Marek’s disease virus serotype 1 (MDV-1) in virus replication was investigated. Deletion of either open reading frame in an infectious bacterial artificial chromosome clone (BAC20) of MDV-1 resulted in progeny viruses that were unable to spread from cell to cell. After transfection of UL10- or UL49.5-negative BAC20 DNA into chicken or quail cells, only single infected cells were observed by indirect immunofluorescence analysis. In contrast, plaque formation was restored when mutant BAC DNAs were co-transfected with the corresponding expression plasmid encoding either the UL10-encoded gM or the UL49.5 gene product. These data demonstrate that gM and its putative complex partner, the UL49.5 homologous protein, are essential for MDV-1 growth in cultured cells. Thus, MDV-1 represents the first example of a member of the family for which the highly conserved membrane proteins are indispensable for cell-to-cell spread.

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© Microbiology Society
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2002-05-01
2024-04-18
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The products of the UL10 (gM) and the UL49.5 genes of Marek’s disease virus serotype 1 are essential for virus growth in cultured cells
J. Gen. Virol. 83, 997 (2002); https://doi.org/10.1099/0022-1317-83-5-997
/content/journal/jgv/10.1099/0022-1317-83-5-997
/content/journal/jgv/10.1099/0022-1317-83-5-997
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