1887

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Volume 96, Issue 3

Temporal expression profiling of novel nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells Free

Abstract

Baculoviruses are arthropod-specific pathogens, and find extensive applications in pest control strategies and recombinant protein expression. nucleopolyhedrovirus (SpltNPV) infects the tropical armyworm , which is an important polyphagous crop pest widely distributed in regions of Asia and Oceania. Using next-generation sequencing, we report stage-specific profiling of SpltNPV-encoded microRNAs (miRNAs) at different time intervals post-infection (p.i.) of Sf21 cells. Sequence length distribution analysis of the small RNA libraries revealed a significant increase in 20 nt reads and a reduction of other size fractions during late phases of infection. miRNA prediction tools identified 48 novel SpltNPV-encoded miRNAs, of which 10 were validated experimentally in Sf21 cells using Northern blot analysis and TaqMan quantitative real-time (qRT)-PCR. The viral miRNAs were also found to be expressed in fat-body and mid-gut tissues of infected fifth-instar larva. qRT-PCR analysis confirmed that expression of most viral miRNAs was triggered 12 h p.i. and continued thereafter. Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotation of computationally predicted targets of the reported miRNAs suggested a major impact of these miRNAs on cell signalling, protein translation and metabolic processes.

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© 2015 The Authors

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Erratum: Temporal expression profiling of novel nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells
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2015-03-01
2024-04-20
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Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells
J. Gen. Virol. 96, 688 (2015); https://doi.org/10.1099/jgv.0.000008
/content/journal/jgv/10.1099/jgv.0.000008
/content/journal/jgv/10.1099/jgv.0.000008
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