1887

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Volume 96, Issue 4

Molecular and immunological characterization of a DNA-launched yellow fever virus 17D infectious clone Free

Abstract

Yellow fever virus (YFV)-17D is an empirically developed, highly effective live-attenuated vaccine that has been administered to human beings for almost a century. YFV-17D has stood as a paradigm for a successful viral vaccine, and has been exploited as a potential virus vector for the development of recombinant vaccines against other diseases. In this study, a DNA-launched YFV-17D construct (pBeloBAC-FLYF) was explored as a new modality to the standard vaccine to combine the commendable features of both DNA vaccine and live-attenuated viral vaccine. The DNA-launched YFV-17D construct was characterized extensively both in cell culture and in mice. High titres of YFV-17D were generated upon transfection of the DNA into cells, whereas a mutant with deletion in the capsid-coding region (pBeloBAC-YF/ΔC) was restricted to a single round of infection, with no release of progeny virus. Homologous prime–boost immunization of AAD mice with both pBeloBAC-FLYF and pBeloBAC-YF/ΔC elicited specific dose-dependent cellular immune response against YFV-17D. Vaccination of A129 mice with pBeloBAC-FLYF resulted in the induction of YFV-specific neutralizing antibodies in all vaccinated subjects. These promising results underlined the potential of the DNA-launched YFV both as an alternative to standard YFV-17D vaccination and as a vaccine platform for the development of DNA-based recombinant YFV vaccines.

  • Received:
  • Accepted:
  • Published Online:
© 2015
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2015-04-01
2024-04-19
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Molecular and immunological characterization of a DNA-launched yellow fever virus 17D infectious clone
J. Gen. Virol. 96, 804 (2015); https://doi.org/10.1099/jgv.0.000026
/content/journal/jgv/10.1099/jgv.0.000026
/content/journal/jgv/10.1099/jgv.0.000026
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