Domain III of NS5A contributes to both RNA replication and assembly of hepatitis C virus particles

References

1. Aoubala M., Holt J., Clegg R. A., Rowlands D. J., Harris M. 2001; The inhibition of cAMP-dependent protein kinase by full-length hepatitis C virus NS3/4A complex is due to ATP hydrolysis. J Gen Virol 82:1637–1646
   [Google Scholar]
2. Appel N., Pietschmann T., Bartenschlager R. 2005; Mutational analysis of hepatitis C virus nonstructural protein 5A: potential role of differential phosphorylation in RNA replication and identification of a genetically flexible domain. J Virol 79:3187–3194 [CrossRef]
   [Google Scholar]
3. Appel N., Zayas M., Miller S., Krijnse-Locker J., Schaller T., Friebe P., Kallis S., Engel U., Bartenschlager R. 2008; Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly. PLoS Pathog 4:e1000035 [CrossRef]
   [Google Scholar]
4. Barba G., Harper F., Harada T., Kohara M., Goulinet S., Matsuura Y., Eder G., Schaff Z., Chapman M. J. other authors 1997; Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc Natl Acad Sci U S A 94:1200–1205 [CrossRef]
   [Google Scholar]
5. Harris M., Coates K. 1993; Identification of cellular proteins that bind to the human immunodeficiency virus type 1 nef gene product in vitro : a role for myristylation. J Gen Virol 74:1581–1589 [CrossRef]
   [Google Scholar]
6. Jones D. M., Gretton S. N., McLauchlan J., Targett-Adams P. 2007; Mobility analysis of an NS5A-GFP fusion protein in cells actively replicating hepatitis C virus subgenomic RNA. J Gen Virol 88:470–475 [CrossRef]
   [Google Scholar]
7. Katze M. G., Kwieciszewski B., Goodlett D. R., Blakely C. M., Neddermann P., Tan S. L., Aebersold R. 2000; Ser(2194) is a highly conserved major phosphorylation site of the hepatitis C virus nonstructural protein NS5A. Virology 278:501–513 [CrossRef]
   [Google Scholar]
8. Kuiken C., Yusim K., Boykin L., Richardson R. 2005; The Los Alamos hepatitis C sequence database. Bioinformatics 21:379–384 [CrossRef]
   [Google Scholar]
9. Macdonald A., Crowder K., Street A., McCormick C., Saksela K., Harris M. 2003; The hepatitis C virus NS5A protein inhibits activating protein-1 (AP1) function by perturbing Ras–ERK pathway signalling. J Biol Chem 278:17775–17784 [CrossRef]
   [Google Scholar]
10. Masaki T., Suzuki R., Murakami K., Aizaki H., Ishii K., Murayama A., Date T., Matsuura Y., Miyamura T. other authors 2008; Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles. J Virol 82:7964–7976 [CrossRef]
    [Google Scholar]
11. McCormick C. J., Brown D., Griffin S., Challinor L., Rowlands D. J., Harris M. 2006a; A link between translation of the hepatitis C virus polyprotein and polymerase function; possible consequences for hyperphosphorylation of NS5A. J Gen Virol 87:93–102 [CrossRef]
    [Google Scholar]
12. McCormick C. J., Maucourant S., Griffin S., Rowlands D. J., Harris M. 2006b; Tagging of NS5A expressed from a functional hepatitis C virus replicon. J Gen Virol 87:635–640 [CrossRef]
    [Google Scholar]
13. Miyanari Y., Atsuzawa K., Usuda N., Watashi K., Hishiki T., Zayas M., Bartenschlager R., Wakita T., Hijikata M., Shimotohno K. 2007; The lipid droplet is an important organelle for hepatitis C virus production. Nat Cell Biol 9:1089–1097 [CrossRef]
    [Google Scholar]
14. Moradpour D., Evans M. J., Gosert R., Yuan Z., Blum H. E., Goff S. P., Lindenbach B. D., Rice C. M. 2004; Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes. J Virol 78:7400–7409 [CrossRef]
    [Google Scholar]
15. Neddermann P., Quintavalle M., Di Pietro C., Clementi A., Cerretani M., Altamura S., Bartholomew L., De Francesco R. 2004; Reduction of hepatitis C virus NS5A hyperphosphorylation by selective inhibition of cellular kinases activates viral RNA replication in cell culture. J Virol 78:13306–13314 [CrossRef]
    [Google Scholar]
16. Pietschmann T., Kaul A., Koutsoudakis G., Shavinskaya A., Kallis S., Steinmann E., Abid K., Negro F., Dreux M. other authors 2006; Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras. Proc Natl Acad Sci U S A 103:7408–7413 [CrossRef]
    [Google Scholar]
17. Quintavalle M., Sambucini S., Summa V., Orsatti L., Talamo F., De F. R., Neddermann P. 2007; Hepatitis C virus NS5A is a direct substrate of casein kinase I- α , a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors. J Biol Chem 282:5536–5544 [CrossRef]
    [Google Scholar]
18. Reed K. E., Xu J., Rice C. M. 1997; Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo : properties of the NS5A-associated kinase. J Virol 71:7187–7197
    [Google Scholar]
19. Shepard C. W., Finelli L., Alter M. J. 2005; Global epidemiology of hepatitis C virus infection. Lancet Infect Dis 5:558–567 [CrossRef]
    [Google Scholar]
20. Tanji Y., Kaneko T., Satoh S., Shimotohno K. 1995; Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A. J Virol 69:3980–3986
    [Google Scholar]
21. Tellinghuisen T. L., Marcotrigiano J., Gorbalenya A. E., Rice C. M. 2004; The NS5A protein of hepatitis C virus is a zinc metalloprotein. J Biol Chem 279:48576–48587 [CrossRef]
    [Google Scholar]
22. Tellinghuisen T. L., Marcotrigiano J., Rice C. M. 2005; Structure of the zinc-binding domain of an essential component of the hepatitis C virus replicase. Nature 435:374–379 [CrossRef]
    [Google Scholar]
23. Tellinghuisen T. L., Foss K. L., Treadaway J. 2008a; Regulation of hepatitis C virion production via phosphorylation of the NS5A protein. PLoS Pathog 4:e1000032 [CrossRef]
    [Google Scholar]
24. Tellinghuisen T. L., Foss K. L., Treadaway J. C., Rice C. M. 2008b; Identification of residues required for RNA replication in domains II and III of the hepatitis C virus NS5A protein. J Virol 82:1073–1083 [CrossRef]
    [Google Scholar]
25. Wakita T., Pietschmann T., Kato T., Date T., Miyamoto M., Zhao Z., Murthy K., Habermann A., Kräusslich H. G. other authors 2005; Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 11:791–796 [CrossRef]
    [Google Scholar]