1887

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Volume 93, Issue 1

Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding Free

Abstract

The dengue virus (DENV) envelope (E) protein mediates virus entry into cells via interaction with a range of cell-surface receptor molecules. Cell-surface glycosaminoglycans (GAGs) have been shown to play an early role in this interaction, and charged oligosaccharides such as heparin bind to the E protein. We have examined this interaction using site-directed mutagenesis of a recombinant form of the putative receptor-binding domain III of the DENV-2E protein expressed as an MBP (maltose-binding protein)-fusion protein. Using an ELISA-based GAG-binding assay, cell-based binding analysis and antiviral-activity assays, we have identified two critical residues, K291 and K295, that are involved in GAG interactions. These studies have also demonstrated differential binding between mosquito and human cells.

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© 2012 SGM
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2012-01-01
2024-04-18
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Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding
J. Gen. Virol. 93, 72 (2012); https://doi.org/10.1099/vir.0.037317-0
/content/journal/jgv/10.1099/vir.0.037317-0
/content/journal/jgv/10.1099/vir.0.037317-0
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