1887

Abstract

Until June 2004, thirty-eight scrapie cases with unusual features, designated Nor98, have been diagnosed in Norway. This study investigated the distribution of PrP genotypes among Nor98 cases, their flock-mates and a random sample of Norwegian slaughtered sheep. The PrP genotype distribution of Nor98 cases differed markedly from that of previous cases of classical scrapie. A leucine/phenylalanine polymorphism at codon 141 with hitherto unknown significance to scrapie was strongly associated with Nor98 cases. Twenty of 38 (52·6 %) cases were either homozygous or heterozygous for phenylalanine at codon 141. In contrast, this allele was present in only 10·5 % of the flock-mates and 4·5 % of the random sample of slaughtered sheep. Moreover, the H allele was represented in 24 of 38 (63·2 %) of Nor98 cases, as opposed to 27·0 % of Nor98 flock-mates and 17·0 % of the slaughtered sheep.

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2005-01-01
2024-04-19
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References

  1. Acín C., Martín-Burriel I., Goldmann W. 7 other authors 2004; Prion protein gene polymorphisms in healthy and scrapie-affected Spanish sheep. J Gen Virol 85:2103–2110 [CrossRef]
    [Google Scholar]
  2. Baylis M., Goldmann W., Houston F., Cairns D., Chong A., Ross A., Smith A., Hunter N., McLean A. R. 2002a; Scrapie epidemic in a fully PrP-genotyped sheep flock. J Gen Virol 83:2907–2914
    [Google Scholar]
  3. Baylis M., Houston F., Kao R. R., McLean A. R., Hunter N., Gravenor M. B. 2002b; BSE – a wolf in sheep's clothing?. Trends Microbiol 10:563–570 [CrossRef]
    [Google Scholar]
  4. Benestad S. L., Sarradin P., Thu B., Schönheit J., Tranulis M. A., Bratberg B. 2003; Cases of scrapie with unusual features in Norway and the designation of a new type, Nor98. Vet Rec 153:202–208 [CrossRef]
    [Google Scholar]
  5. Bossers A., Schreuder B. E. C., Muileman I. H., Belt P. B. G. M., Smits M. A. 1996; PrP genotype contributes to determining survival times of sheep with natural scrapie. J Gen Virol 77:2669–2673 [CrossRef]
    [Google Scholar]
  6. Buschmann A., Biacabe A.-G., Ziegler U., Bencsik A., Madec J.-Y., Erhardt G., Lühken G., Baron T., Groschup M. H. 2004; Atypical scrapie cases in Germany and France are identified by discrepant reaction patterns in BSE rapid tests. J Virol Methods 117:27–36 [CrossRef]
    [Google Scholar]
  7. Dawson M., Hoinville L. J., Hosie B. D., Hunter N. 1998; Guidance on the use of PrP genotyping as an aid to the control of clinical scrapie. Vet Rec 142:623–625
    [Google Scholar]
  8. Elsen J.-M., Amigues Y., Schelcher F. 7 other authors 1999; Genetic susceptibility and transmission factors in scrapie: detailed analysis of an epidemic in a closed flock of Romanov. Arch Virol 144:431–445 [CrossRef]
    [Google Scholar]
  9. Goldmann W., Hunter N., Smith G., Foster J., Hope J. 1994; PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. J Gen Virol 75:989–995 [CrossRef]
    [Google Scholar]
  10. Heaton M. P., Leymaster K. A., Freking B. A. 7 other authors 2003; Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer. Mamm Genome 14:765–777 [CrossRef]
    [Google Scholar]
  11. Hopp P., Ulvund M. J., Jarp J. 2001; A case-control study on scrapie in Norwegian sheep flocks. Prev Vet Med 51:183–198 [CrossRef]
    [Google Scholar]
  12. Hunter N. 1997; PrP genetics in sheep and the implications for scrapie and BSE. Trends Microbiol 5:331–334 [CrossRef]
    [Google Scholar]
  13. Hunter N., Goldmann W., Smith G., Hope J. 1994; The association of a codon 136 PrP gene variant with the occurrence of natural scrapie. Arch Virol 137:171–177 [CrossRef]
    [Google Scholar]
  14. Hunter N., Foster J. D., Goldmann W., Stear M. J., Hope J., Bostock C. 1996; Natural scrapie in a closed flock of Cheviot sheep occurs only in specific PrP genotypes. Arch Virol 141:809–824 [CrossRef]
    [Google Scholar]
  15. Kutzer T., Pfeiffer I., Brenig B. 2002; Identification of new allelic variants in the ovine prion protein (PrP) gene. J Anim Breed Genet 119:201–208 [CrossRef]
    [Google Scholar]
  16. Lühken G., Buschmann A., Groschup M. H., Erhardt G. 2004; Prion protein allele A136H154Q171 is associated with high susceptibility to scrapie in purebred and crossbred German Merinoland sheep. Arch Virol 149:1571–1580
    [Google Scholar]
  17. O'Doherty E., Healy A., Aherne M., Hanrahan J. P., Weavers E., Doherty M., Roche J. F., Gunn M., Sweeney T. 2002; Prion protein (PrP) gene polymorphisms associated with natural scrapie cases and their flock-mates in Ireland. Res Vet Sci 73:243–250 [CrossRef]
    [Google Scholar]
  18. O'Rourke K. I., Holyoak G. R., Clark W. W., Mickelson J. R., Wang S., Melco R. P., Besser T. E., Foote W. C. 1997; PrP genotypes and experimental scrapie in orally inoculated Suffolk sheep in the United States. J Gen Virol 78:975–978
    [Google Scholar]
  19. Thorgeirsdottir S., Sigurdarson S., Thorisson H. M., Georgsson G., Palsdottir A. 1999; PrP gene polymorphism and natural scrapie in Icelandic sheep. J Gen Virol 80:2527–2534
    [Google Scholar]
  20. Tranulis M. A., Osland A., Bratberg B., Ulvund M. J. 1999; Prion protein gene polymorphisms in sheep with natural scrapie and healthy controls in Norway. J Gen Virol 80:1073–1077
    [Google Scholar]
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