1887

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Volume 86, Issue 4

Gene organization and sequencing of the defective nucleopolyhedrovirus genome Free

Abstract

Two distinct nucleopolyhedrovirus species of the eastern spruce budworm, , exist in a symbiont-like relationship. defective nucleopolyhedrovirus (CfDEFNPV) only infects larvae in the presence of nucleopolyhedrovirus Ireland strain (CfMNPV), but is infective when injected into the haemolymph. CfDEFNPV synergizes CfMNPV in infections and CfMNPV is always the predominant progeny. This study was undertaken to report the genomic makeup and organization of CfDEFNPV in an attempt to identify its defect and understand its synergistic role. The genome was mapped, sequenced, characterized and compared to other baculoviruses. The CfDEFNPV genome was 131 160 nt long with 149 putative open reading frames (ORFs) and a G+C content of 45·8 mol%. Homologues of all 62 conserved lepidopteran baculovirus genes were found including those implicated in infectivity, , () and . Although no obvious deletions were observed to explain the defect, two ORFs, Cfdef79 and Cfdef99 (), contained potential deletions. Cfdef50 ()/Cfdef51 () and Cfdef76/Cfdef77 () had large overlaps and a potential homologue to ac105/ was split. Four baculovirus repeat ORFs were present, as were two unique genes, but no enhancins were identified. CfDEFNPV contained 13 homologous regions, each with one to five palindromes. Comparison with fully sequenced baculovirus genomes identified CfDEFNPV as a group I NPV with the closest average amino acid identity to , followed by and CfMNPV, with its closest matches being to individual gene sequences.

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  • Accepted:
  • Published Online:
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2005-04-01
2024-04-25
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Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome
J. Gen. Virol. 86, 945 (2005); https://doi.org/10.1099/vir.0.80489-0
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