1887

Abstract

Susceptibility to scrapie in sheep is influenced by polymorphisms of the prion protein () gene, whereas no strong association between genetics and scrapie has yet been determined in goats due to the limited number of studies on these animals. In this case–control study on 177 goats from six Italian scrapie outbreaks, the association between alleles and the occurrence of scrapie was studied. Three silent mutations and 11 polymorphisms were identified, of which two polymorphisms (L133Q and M137I) and one silent mutation (T202T) have not been reported previously. Twelve alleles were determined by cloning. Statistical analysis suggested a possible protective role against scrapie for the glutamine to lysine mutation at codon 222.

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2006-04-01
2024-03-28
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References

  1. Acutis P. L., Sbaiz L., Verburg F., Riina M. V., Ru G., Moda G., Caramelli M., Bossers A. 2004; Low frequency of the scrapie resistance-associated allele and presence of lysine-171 allele of the prion protein gene in Italian Biellese ovine breed. J Gen Virol 85:3165–3172 [CrossRef]
    [Google Scholar]
  2. Agrimi U., Ru G., Cardone F., Pocchiari M., Caramelli M. 1999; Epidemic of transmissible spongiform encephalopathy in sheep and goats in Italy. Lancet 353:560–561
    [Google Scholar]
  3. Agrimi U., Conte M., Morelli L. & 9 other authors 2003; Animal transmissible spongiform encephalopathies and genetics. Vet Res Commun 27 (Suppl. 1):31–38 [CrossRef]
    [Google Scholar]
  4. Bartz J. C., McKenzie D. I., Bessen R. A., Marsh R. F., Aiken J. M. 1994; Transmissible mink encephalopathy species barrier effect between ferret and mink: PrP gene and protein analysis. J Gen Virol 75:2947–2953 [CrossRef]
    [Google Scholar]
  5. Baylis M., Goldmann W. 2004; The genetics of scrapie in sheep and goats. Curr Mol Med 4:385–396 [CrossRef]
    [Google Scholar]
  6. Belt P. B. G. M., Muileman I. H., Schreuder B. E. C., Bos-de Ruijter J., Gielkens A. L. J., Smits M. A. 1995; Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. J Gen Virol 76:509–517 [CrossRef]
    [Google Scholar]
  7. Billinis C., Panagiotidis C. H., Psychas V., Argyroudis S., Nicolaou A., Leontides S., Papadopoulos O., Sklaviadis T. 2002; Prion protein gene polymorphisms in natural goat scrapie. J Gen Virol 83:713–721
    [Google Scholar]
  8. Bossers A., Schreuder B. E. C., Muileman I. H., Belt P. B. G. M., Smits M. A. 1996; PrP genotype contributes to determining survival times of sheep with natural scrapie. J Gen Virol 77:2669–2673 [CrossRef]
    [Google Scholar]
  9. Bossers A., de Vries R., Smits M. A. 2000; Susceptibility of sheep for scrapie as assessed by in vitro conversion of nine naturally occurring variants of PrP. J Virol 74:1407–1414 [CrossRef]
    [Google Scholar]
  10. Capucchio M. T., Guarda F., Isaia M. C., Caracappa S., Di Marco V. 1998; Natural occurrence of scrapie in goats in Italy. Vet Rec 143:452–453 [CrossRef]
    [Google Scholar]
  11. Caramelli M., Ru G., Casalone C., Bozzetta E., Acutis P. L., Calella A., Forloni G. 2001; Evidence for the transmission of scrapie to sheep and goats from a vaccine against Mycoplasma agalactiae . Vet Rec 148:531–536 [CrossRef]
    [Google Scholar]
  12. Dawson M., Hoinville L. J., Hosie B. D., Hunter N. 1998; Guidance on the use of PrP genotyping as an aid to the control of clinical scrapie. Scrapie Information Group. Vet Rec 142:623–625
    [Google Scholar]
  13. Goldmann W., Martin T., Foster J., Hughes S., Smith G., Hughes K., Dawson M., Hunter N. 1996; Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period. J Gen Virol 77:2885–2891 [CrossRef]
    [Google Scholar]
  14. Goldmann W., Chong A., Foster J., Hope J., Hunter N. 1998; The shortest known prion protein gene allele occurs in goats, has only three octapeptide repeats and is non-pathogenic. J Gen Virol 79:3173–3176
    [Google Scholar]
  15. Goldmann W., Perucchini M., Smith A., Hunter N. 2004; Genetic variability of the PrP gene in a goat herd in the UK. Vet Rec 155:177–178 [CrossRef]
    [Google Scholar]
  16. Gombojav A., Ishiguro N., Horiuchi M., Serjmyadag D., Byambaa B., Shinagawa M. 2003; Amino acid polymorphisms of PrP gene in Mongolian sheep. J Vet Med Sci 65:75–81 [CrossRef]
    [Google Scholar]
  17. Hunter N., Foster J. D., Goldmann W., Stear M. J., Hope J., Bostock C. 1996; Natural scrapie in a closed flock of Cheviot sheep occurs only in specific PrP genotypes. Arch Virol 141:809–824 [CrossRef]
    [Google Scholar]
  18. Hunter N., Goldmann W., Foster J. D., Cairns D., Smith G. 1997; Natural scrapie and PrP genotype: case-control studies in British sheep. Vet Rec 141:137–140 [CrossRef]
    [Google Scholar]
  19. Kurosaki Y., Ishiguro N., Horiuchi M., Shinagawa M. 2005; Polymorphisms of caprine PrP gene detected in Japan. J Vet Med Sci 67:321–323 [CrossRef]
    [Google Scholar]
  20. Shibuya S., Higuchi J., Shin R. W., Tateishi J., Kitamoto T. 1998; Codon 219 Lys allele of PRNP is not found in sporadic Creutzfeldt–Jakob disease. Ann Neurol 43:826–828 [CrossRef]
    [Google Scholar]
  21. Thorgeirsdottir S., Sigurdarson S., Thorisson H. M., Georgsson G., Palsdottir A. 1999; PrP gene polymorphism and natural scrapie in Icelandic sheep. J Gen Virol 80:2527–2534
    [Google Scholar]
  22. Vascellari M., Aufiero G. M., Nonno R. & 7 other authors 2005; Diagnosis and PrP genotype target of scrapie in clinically healthy sheep of Massese breed in the framework of a scrapie eradication programme. Arch Virol 150:1959–1976 [CrossRef]
    [Google Scholar]
  23. Wopfner F., Weidenhöfer G., Schneider R., von Brunn A., Gilch S., Schwarz T. F., Werner T., Schätzl H. M. 1999; Analysis of 27 mammalian and 9 avian PrPs reveals high conservation of flexible regions of the prion protein. J Mol Biol 289:1163–1178 [CrossRef]
    [Google Scholar]
  24. Zhang L., Li N., Fan B., Fang M., Xu W. 2004; PRNP polymorphisms in Chinese ovine, caprine and bovine breeds. Anim Genet 35:457–461 [CrossRef]
    [Google Scholar]
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