1887

Abstract

SUMMARY

HeLa cells infected with radioactive poliovirus type 2 were disrupted with ultrasonic treatment, followed by addition of a non-ionic detergent. Two types of virus particles were found to sediment at 80 to 90% the rate of native virus. The first of these appeared to be a complex of native virus particles and membrane components, since treatment with 0.2% SDS released infectious native particles. The second was non-infectious and its sedimentation rate was not greatly altered by . One hour after infection this non-infectious particle was the major product of cell-mediated eclipse.

We have confirmed that 10 to 30 μg/ml -7, a substituted thiopyrimidine, blocks infection of cells by poliovirus in a specific manner. Analysis of cells infected with radioactive poliovirus type 2 in the presence of -7 showed that the virus particles remained as the complex which can be disrupted with . In addition to blocking cell-mediated eclipse, -7 stabilizes poliovirus against heat inactivation at the same concentrations which block infection. This action resembles the effect of 10 -glutathione, which is also known to block cell-mediated eclipse of poliovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-27-3-329
1975-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/27/3/JV0270030329.html?itemId=/content/journal/jgv/10.1099/0022-1317-27-3-329&mimeType=html&fmt=ahah

References

  1. Briendl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  2. Butterworth B. E. 1973; A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus 1A, and poliovirus. Virology 56:439–453
    [Google Scholar]
  3. Chi Y. F., Wu Y.-L. 1957; The action of amines and hydrazine on ethyl 2-methylthio-4-methyl pyrimidine5-carboxylate. Hua Hsüeh Hsüeh Pao 23:145–149 ACS Chemical Abstracts 52, 14626
    [Google Scholar]
  4. Crowell R. L., Philipson L. 1971; Specific alterations of Coxsackie B3 eluted from HeLa cells. Journal of Virology 8:509–515
    [Google Scholar]
  5. Eggers H. J. 1970; Inhibition of early stages of virus-cell interaction. Annals of the New York Academy of Sciences 173:417–419
    [Google Scholar]
  6. Fenwick M. L., Cooper P. D. 1962; Early interactions between poliovirus and ERK cells. Some observations on the nature and significance of the rejected particles. Virology 18:212–223
    [Google Scholar]
  7. Fenwick M. L., Wall M. J. 1973; Factors determining the site of synthesis of poliovirus proteins: the early attachment of virus particles to endoplasmic membranes. Journal of Cell Science 13:403–413
    [Google Scholar]
  8. Habermehl K.-O., Diefenthal W., Bucholz M. 1974; Distribution of parental viral constituents in the course of polio infection, in. Advances in the Biosciences 11 pp. 41–64 Edited by Raspe G. Oxford: Pergamon Press;
    [Google Scholar]
  9. Halsted C. C., Seto D. S. Y., Simkins J., Carver D. H. 1970; Protection of enteroviruses against heat inactivation by sulfhydryl reducing substances. Virology 40:751–754
    [Google Scholar]
  10. Holland J. J. 1962; Irreversible eclipse of poliovirus by HeLa cells. Virology 16:163–176
    [Google Scholar]
  11. Hirst G. K. 1960; Reversible inactivation of poliovirus through exposure to sulfhydryl compounds. International Poliomyelitis Conference 5th (Copenhagen) pp. 11–12
    [Google Scholar]
  12. Joklik N. K., Darnell J. E. 1961; The adsorption and early fate of purified poliovirus in HeLa cells. Virology 13:439–447
    [Google Scholar]
  13. Korant B. D., Lonberg-Holm K., Noble J., Stasny J. T. 1972; Naturally occurring and artificially produced components of three rhinoviruses. Virology 48:71–86
    [Google Scholar]
  14. Lacolla P., Marcialis M. A., Mereu G. P., Loddo B. 1972; Specific inhibition of poliovirus induced blockade of cell protein synthesis by a thiopyrimidine derivative. Journal of General Virology 17:13–18
    [Google Scholar]
  15. Lonberg-Holm K., Korant B. D. 1972; Early interaction of rhinoviruses with host cells. Journal of Virology 9:29–40
    [Google Scholar]
  16. Lonberg-Holm K., Noble-Harvey J. 1973; Comparison of in vitro and cell-mediated alteration of a human rhinovirus inhibition by sodium dodecyl sulfate. Journal of Virology 12:819–826
    [Google Scholar]
  17. Lonberg-Holm K., Philipson L. 1974 Early interaction between animal viruses and cells. In Monographs in Virology 9 pp. 1–148 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  18. Mandel B. 1962; The use of sodium dodecyl sulfate in studies on the interaction of poliovirus and HeLa cells. Virology 17:288–294
    [Google Scholar]
  19. Mandel B. 1967; The relationship between penetration and uncoating of poliovirus in HeLa cells. Virology 31:702–712
    [Google Scholar]
  20. Pohjanpelto P. 1958; Stabilization of poliovirus by cystine. Virology 6:472–487
    [Google Scholar]
  21. Steele F. M., Black F. L. 1967; Inactivation and heat stabilization of poliovirus by 2-thiouracil. Journal of Virology 1:653–658
    [Google Scholar]
  22. Yamazi Y., Miyanaga Y., Todome Y., Sekiya S., Akamatsu H. 1966; Inhibition of cytopathic effect and multiplication of poliovirus by ethyl-2-methylthio-4-methyl-5-pyrimidine-carboxylate (S-7) and ethyl-2ethylthio-4-methyl-5-pyrimidine-carboxylate (S-9). Nippon Kagaku Ryohogakukai Zasshi 14:444–448
    [Google Scholar]
  23. Yamazi Y., Takahashi M., Todome Y. 1970; Inhibition of poliovirus by effect of a methylthiopyrimidine derivative. Proceedings of the Society for Experimental Biology and Medicine 133:674–677
    [Google Scholar]
  24. Zajac I., Crowell R. c. 1969; Differential inhibition of attachment and eclipse activities of HeLa cells for enteroviruses. Journal of Virology 3:422–428
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-27-3-329
Loading
/content/journal/jgv/10.1099/0022-1317-27-3-329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error