1887

Abstract

SUMMARY

Interferon production in Namalwa cells, a human lymphoblastoid line, was enhanced by lowering the incubation temperature after induction. The optimum conditions for this effect were established. At the lower temperature interferon synthesis proceeded at a lower rate but continued for longer. Interferon mRNA was shown to be associated with the polysomes for longer after induction. Addition of drugs that inhibit transcription did not prevent the increased production of interferon. Thus, the increased production of interferon is due to the prolonged translation of interferon mRNA.

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1981-09-01
2024-04-25
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References

  1. Adolf G. R., Swetly P. 1979; Interferon production by human lymphoblastoid cells is stimulated by inducers of Friend cell differentiation. Virology 99:158–166
    [Google Scholar]
  2. Allen G., Fantes K. H. 1980; A family of structural genes for human lymphoblastoid (leukocyte-type) interferon. Nature, London 287:408–411
    [Google Scholar]
  3. Atherton K. T., Burke D. C. 1975; Interferon induction by viruses and polynucleotides: a differential effect of camptothecin. Journal of General Virology 29:297–304
    [Google Scholar]
  4. Baker P. N., Bradshaw T. K., Morser J., Burke D. C. 1979; The effect of 5-bromodeoxyuridine on interferon production in human cells. Journal of General Virology 45:177–184
    [Google Scholar]
  5. Baker P. N., Morser J., Burke D. C. 1980; Effects of sodium butyrate on a human lymphoblastoid cell line (Namalwa) and its interferon production. Journal of Interferon Research 1:71–77
    [Google Scholar]
  6. Berger S. L., Hitchcock M. J. M., Zoon K. C., Birkenmeir C. S., Friedman R. M., Chang E. H. 1980; Characterization of interferon messenger RNA synthesis in Namalwa cells. Journal of Biological Chemistry 255:2955–2961
    [Google Scholar]
  7. Cavalieri R. L., Havell E. A., Vilcek J., Pestka S. 1977; Induction and decay of human fibroblast interferon mRNA. Proceedings of the National Academy of Sciences of the United States of America 74:4415–4419
    [Google Scholar]
  8. Colman A., Morser J. 1979; Export of proteins from oocytes of Xenopus laevis . Cell 17:517–526
    [Google Scholar]
  9. Colman A., Morser J., Valle G., Lane C., Besley J. 1981; The involvement of the cytoskeleton in protein secretion by Xenopus laevis oocytes. Journal of Cell Biology (in press)
    [Google Scholar]
  10. Havell E. A., Vilcek J. 1973; Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature. Proceedings of the National Academy of Sciences of the United States of America 70:3909–3913
    [Google Scholar]
  11. Havell E. A., Yip Y. K., Vilcek J. 1977; Characteristics of human lymphoblastoid (Namalwa) interferon. Journal of General Virology 38:51–59
    [Google Scholar]
  12. Interferon Nomenclature 1980 Nature, London 286:110
    [Google Scholar]
  13. Johnston M. D. 1980; Enhanced production of interferon from human lymphoblastoid (Nawalwa) cells pre-treated with sodium butyrate. Journal of General Virology 50:191–194
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Morser J., Colman A. 1980; Post-translational events in the production of human lymphoblastoid interferon. Journal of General Virology 51:117–124
    [Google Scholar]
  16. Morser J., Flint J., Meager A., Graves H., Baker P. N., Colman A., Burke D. C. 1979; Characterization of interferon messenger RNA from human lymphoblastoid cells. Journal of General Virology 44:231–234
    [Google Scholar]
  17. Morser J., Meager A., Colman A. 1980; Enhancement of interferon mRNA levels in butyric-acid treated Namalwa cells. FEBS Letters 112:203–206
    [Google Scholar]
  18. Secher D. S. 1981; An immunoradiometric assay for human leucocyte interferon using monoclonal antibody. Nature, London 290:501–503
    [Google Scholar]
  19. Secher D. S., Burke D. C. 1980; A monoclonal antibody for large scale purification of human leukocyte interferon. Nature, London 285:446–450
    [Google Scholar]
  20. Stewart W. E. II 1979 The Interferon System Vienna and New York: Springer-Verlag;
    [Google Scholar]
  21. Tovey M. G., Begon-Lours J., Gresser I., Morris A. 1977; Marked enhancement of interferon production in 5-bromodeoxyuridine treated human lymphoblastoid cells. Nature, London 267:455–457
    [Google Scholar]
  22. Zoon K. C., Buckler C. E., Bridgen P. J., Gurari-Rotman D. 1978; Production of human lymphoblastoid interferon by Namalwa cells. Journal of Clinical Microbiology 7:44–51
    [Google Scholar]
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