1887

Abstract

SUMMARY

Turnip rosette virus (TRosV) and its RNA have been entrapped in multilamellar vesicles (MLVs) composed of phosphatidylcholine and β-sitosterol (neutral MLVs) or of phosphatidylcholine and sterylamine (positively charged MLVs). TRosV RNA was also entrapped in vesicles of phosphatidylcholine, β-sitosterol and dicetylphosphate prepared by the reverse phase evaporation method (REVs). TRosV in positively charged or neutral MLVs will infect turnip protoplasts in the presence of fusogenic agents (polyethylene glycol and/or CaCl); TRosV RNA in such MLVs did not infect protoplasts. TRosV RNA in REVs infected protoplasts whereas the unentrapped RNA did not.

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1982-06-01
2024-04-24
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References

  1. Bangham A. D., Standish M. M., Watkins J. C. 1965; Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of molecular biology 13:238–252
    [Google Scholar]
  2. Cassells A. C. 1978; Uptake of charged lipid vesicles by isolated tomato protoplasts. Nature, London 275:760
    [Google Scholar]
  3. Dawson J. R. O., Dickerson P. E., King J. M., Sakai F., Trim A. R. H., Watts J. W. 1978; Improved methods for infection of plant protoplasts with viral ribonucleic acid. Zeitschrift für Naturforschung 33 c:548–551
    [Google Scholar]
  4. Denloye A. O., Homer R. B., Hull R. 1978; Circular dichroism studies on turnip rosette virus. Journal of General Virology 41:77–85
    [Google Scholar]
  5. Fraley R., Subramani S., Berg P., Papahadjopoulos D. 1980; Introduction of liposome-encapsulated SV40 DNA into cells. Journal of Biological Chemistry 255:10431–10435
    [Google Scholar]
  6. Fukunaga Y., Nagata T., Takebe I. 1981; Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology 113:752–760
    [Google Scholar]
  7. Hull R. 1977; The banding behaviour of the viruses of southern bean mosaic virus group in gradients of caesium sulphate. Virology 79:50–57
    [Google Scholar]
  8. Hull R. 1978; The possible use of plant viral DNAs in genetic manipulations in plants. Trends in Biochemical Sciences 3:254–256
    [Google Scholar]
  9. Hull R., Covey S. N., Stanley J., Davies J. W. 1979; The polarity of the cauliflower mosaic virus genome. Nucleic Acids Research 7:669–677
    [Google Scholar]
  10. Lurquin P. F. 1979; Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts. Nucleic Acids Research 6:3773–3784
    [Google Scholar]
  11. Lurquin P. F. 1981; Finding of plasmid loaded liposomes to plant protoplasts. Validity of biochemical methods to evaluate the transfer of exogenous DNA. Plant Science Letters 21:31–40
    [Google Scholar]
  12. Lurquin P. F., Sheehy R. E., Rao N. A. 1981; Quantitative aspects of nucleic acid sequestration in large liposomes and their effects on plant protoplasts. FEBS Letters 125:183–187
    [Google Scholar]
  13. Maule A. J., Boulton M. I., Edmunds C., Wood K. R. 1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  14. Morris Krsinich B. A. M. 1980 Studies on turnip rosette virus replication Ph.D. thesis University of East Anglia;
    [Google Scholar]
  15. Morris Krsinich B. A. M., Hull R., Russo M. 1979; Infection of turnip leaf protoplasts with turnip rosette virus. Journal of General Virology 43:339–347
    [Google Scholar]
  16. Nagata T., Okada K., Takebe I., Matsui C. 1981; Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Molecular and General Genetics 184:161–165
    [Google Scholar]
  17. Rollo F., Sala F., Cella R., Parisi B. 1980; Liposome-mediated association of DNA with plant protoplasts: influence of vesicle lipid composition. In Plant Cell Cultures: Results and Perspectives pp 237–246 Edited by Sala F., Parisi B., Celia R., Ciferri O. Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  18. Rollo F., Galli M. G., Parisi B. 1981; Liposome-mediated transfer of DNA to carrot protoplasts: a biochemical and autoradiographic analysis. Plant Science Letters 20:347–354
    [Google Scholar]
  19. Szeto W. W., Hamer D. H., Carlson P. S., Thomas C. A. 1977; Cloning of cauliflower mosaic virus (CaMV) DNA in Escherichia coli. Science 196:210–214
    [Google Scholar]
  20. Szoka F. Tr., Papahadjopoulos D. 1978; Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proceedings of the National Academy of Sciences of the United States of America 75:4194–4198
    [Google Scholar]
  21. Taber R., Wilson T., Papahadjopoulos D. 1978; The encapsulation of picornaviruses by lipid vesicles: physical and biological properties. In Liposomes and Their Uses in Biology and Medicine pp 268–274 Edited by Papahadjopoulos D. New York: The New York Academy of Sciences;
    [Google Scholar]
  22. Wilson T., Papahadjopoulos D., Taber R. 1977; Biological properties of poliovirus encapsulated in lipid vesicles: antibody resistance and infectivity in virus-resistant cells. Proceedings of the National Academy of Sciences of the United States of America 74:3471–3475
    [Google Scholar]
  23. Wilson T., Papahadjopoulos D., Taber R. 1979; The introduction of poliovirus RNA into cells via lipid vesicles (liposomes). Cell 17:77–84
    [Google Scholar]
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