%0 Journal Article %A Fuller, Frederick %A Bhown, Ajit S. %A Bishop, David H. L. %T Bunyavirus Nucleoprotein, N, and a Non-structural Protein, NSS, Are Coded by Overlapping Reading Frames in the S RNA %D 1983 %J Journal of General Virology, %V 64 %N 8 %P 1705-1714 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-8-1705 %K snowshoe hare bunyavirus %K bunyavirus mRNA coding assignments %K La Crosse bunyavirus %I Microbiology Society, %X SUMMARY It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 × 103 and 10.5 × 103 respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for nonstructural proteins (NSS and NSM, respectively). In the present report, in vitro translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NSS. Compositional analyses of selected tryptic peptides of N and NSS have provided proof that N is the product of the first open reading frame, and NSS the product of the second. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-8-1705