RT Journal Article SR Electronic(1) A1 Fuller, Frederick A1 Bhown, Ajit S. A1 Bishop, David H. L.YR 1983 T1 Bunyavirus Nucleoprotein, N, and a Non-structural Protein, NSS, Are Coded by Overlapping Reading Frames in the S RNA JF Journal of General Virology, VO 64 IS 8 SP 1705 OP 1714 DO https://doi.org/10.1099/0022-1317-64-8-1705 PB Microbiology Society, SN 1465-2099, AB SUMMARY It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 × 103 and 10.5 × 103 respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for nonstructural proteins (NSS and NSM, respectively). In the present report, in vitro translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NSS. Compositional analyses of selected tryptic peptides of N and NSS have provided proof that N is the product of the first open reading frame, and NSS the product of the second., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-8-1705