1887

Abstract

SUMMARY

Spontaneous osteomas in strain 101 mice, a strain which has a high incidence of benign bone tumours, harbour numerous C-type virus-like particles with pleomorphic characteristics. A cell-free extract from osteomas from two mice induced bone tumours, together with osteopetrosis and lymphomas, in newborn mice of the low incidence NMRI strain after a latent period of 12 to 15 months. When C3H embryo fibroblasts were infected with the osteoma extract, the resulting cell line produced virus (OA MuLV) with a high titre. OA MuLV was cloned by serial endpoint dilution and NIH 3T3 cells were productively infected. The resulting virus was named OA MuLV. OA MuLV and OA MuLV also induced bone tumours, osteopetrosis and lymphomas 12 to 15 months after injection into newborn NMRI mice. The isolated virus showed typical characteristics of the murine retrovirus group. Fv-1 host range restriction assays classified the viruses as N-ecotropic and XC-positive. Tryptic p30 peptide analysis and RNase T fingerprint analysis of OA MuLV and OA MuLV indicated that OA MuLV contains an Akv-like virus as well as additional components, whereas OA MuLV is closely related to Akv, but not identical to it. Serological analysis of the envelope proteins using monoclonal antibodies also showed the virus to be similar, but not identical, to Akv virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-12-2237
1984-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/12/JV0650122237.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-12-2237&mimeType=html&fmt=ahah

References

  1. Amsel S., Dell E. S. 1972; Bone formation by hemopoietic tissue: separation of preosteoblast from hemopoietic stem cell function in the rat. Blood 39:267–272
    [Google Scholar]
  2. Baltimore D., Smoler D. 1971; Primer requirements and template specificity of the DNA polymerase of RNA tumor viruses. Proceedings of the National Academy of Sciences, U.S.A 68:1507–1511
    [Google Scholar]
  3. Bates D. L., Perham R. N., Caggins J. R. 1975; Methods for obtaining peptide maps of proteins on a subnano-mole scale. Analytical Biochemistry 68:175–184
    [Google Scholar]
  4. Breslow N. E., Day N. E., Tomatis L., Turusov V. S. 1974; Association between tumor types in a large-scale carcinogenesis study of CF-1 mice. Journal of the National Cancer Institute 52:233–239
    [Google Scholar]
  5. Charles R. T., Turusov V. S. 1974; Bone tumours in CF-1 mice. Laboratory Animals 8:137–144
    [Google Scholar]
  6. Chen T. R. 1977; In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research 104:255–262
    [Google Scholar]
  7. Duc-Nguyen H. 1968; Enhancing effect of diethylaminoethyldextran on the focus forming titer of a murine sarcoma virus (Harvey strain). Journal of Virology 2:643–644
    [Google Scholar]
  8. Dunn T. B. 1954; Normal and pathologic anatomy of the reticular tissue in laboratory mice, with a classification and discussion of neoplasms. Journal of the National Cancer Institute 14:1281–1433
    [Google Scholar]
  9. Erfle V., Neumann G. 1980; Bacteriological examination of specified-pathogen-free animals: a procedure for small rodents. Laboratory Animals 14:123–126
    [Google Scholar]
  10. Erfle V., Schulte-Overberg S., Marquart K.-H., Adler I.-D., Luz A. 1979; Establishment and characterisation of C-type RNA virus-producing cell lines from radiation-induced murine osteosarcomas. Journal of Cancer Research and Clinical Oncology 94:149–162
    [Google Scholar]
  11. Erfle V., Hehlmann R., Schetters H., Meier A., Luz A. 1980; Time course of C-type retrovirus expression in mice submitted to osteosarcomagenic doses of 224Radium. International Journal of Cancer 26:107–113
    [Google Scholar]
  12. Etzerodt M., Mikkelsen T., Pedersen F. S., Kjeldgaard N. O., Jorgensen P. 1984; The nucleotide sequence of the Akv murine leukemia virus genome. Virology 133: (in press)
    [Google Scholar]
  13. Finkel M. P., Reilly C. A. Jr 1973; Observations suggesting the viral etiology of radiation-induced tumours, particularly osteogenic sarcomas. In Radionucleotide Carcinogenesis pp 278–288 Edited by Sanders C. L., Busch R., Ballon J. E., Mahlum D. D. Oak Ridge, Tenn.: United States Atomic Energy Commission;
    [Google Scholar]
  14. Finkel M. P., Reilly C. A. Jr, Biskis B. O., Greco I. L. 1973; Bone tumour viruses. In Bone – Certain Aspects of Neoplasia (Colston Papers, no. 24), pp 353–364 Edited by Price C. H. G., Ross F. G. M. London: Butterworths;
    [Google Scholar]
  15. Finkel M. P., Reilly C. A. Jr, Biskis B. O. 1976; Pathogenesis of radiation and virus-induced bone tumours. Recent Results in Cancer Research 54:92–103
    [Google Scholar]
  16. Furth J. 1946; Prolongation of life with prevention of leukemia by thymectomy in mice. Journal of Gerontology 1:46–54
    [Google Scholar]
  17. Goodman N. C., Spiegelman S. 1971; Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proceedings of the National Academy of Sciences, U.S.A 67:1789–1796
    [Google Scholar]
  18. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 31I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  19. Holmes J. R. 1964; Avian osteopetrosis. National Cancer Institute Monographs 17:63–79
    [Google Scholar]
  20. Ihle J. N., Yurconic M. Jr, Hanna M. G. Jr 1973; Autogenous immunity to endogenous RNA tumour virus: radioimmunoprecipitation assay of mouse serum antibody levels. Journal of Experimental Medicine 138:194–208
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Lostrom M. E., Stone M. R., Tam M. R., Burnette W. N., Pinter A., Nowinski R. C. 1979; Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the p 15 (E) and gp70 envelope proteins. Virology 98:336–350
    [Google Scholar]
  23. Loutit J. F., Sansom J. M. 1976; Osteopetrosis of microphthalmic mice – a defect of the hematopoietic stem cell?. Calcified Tissue Research 20:251–259
    [Google Scholar]
  24. Loutit J. F., Marshall M. J., Nisbet N. W., Vaughan J. M. 1982; Versatile stem cells in bone marrow. Lancet ii:1090–1093
    [Google Scholar]
  25. Marquart K. H. 1979; Pleomorphic C-type virus particles in bone tissue of strain 101 mice and (C3H × 101) F, hybrids. Calcified Tissue International 28:259–262
    [Google Scholar]
  26. Merwin R. M., Redmon L. W. 1969; Skeletal and reticular tissue disorders produced in mice by agent(s) from sarcoma 37. Journal of the National Cancer Institute 43:365–376
    [Google Scholar]
  27. Nexø B. A. 1977; A plaque assay for murine leukemia virus using enzyme coupled antibodies. Virology 77:849–852
    [Google Scholar]
  28. O’donnell P. V., Nowinski R. 1980; Serologic analysis of antigenic determinants on the env gene products of AKR dual tropic (MCF) murine leukemia viruses. Virology 107:81–88
    [Google Scholar]
  29. Oroszlan S., Foreman C., Kellof G., Gilden R. V. 1971; The group specific antigen and other structural proteins of hamster and mouse C-type viruses. Virology 43:665–674
    [Google Scholar]
  30. Pedersen F. S., Haseltine W. A. 1980; Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA. Journal of Virology 33:349–365
    [Google Scholar]
  31. Pedersen F. S., Buchhagen D. L., Chen C. Y., Hays E. F., Haseltine W. A. 1980; Characterisation of virus produced by a lymphoma induced by inoculation of MCF 247 virus. Journal of Virology 35:211–218
    [Google Scholar]
  32. Pedersen F. S., Crowther R. L., Tenney D. Y., Reimold A. M., Haseltine W. A. 1981; Novel leukemogenic retrovirus isolated from cell line derived from spontaneous AKR tumour. Nature, London 292:167–170
    [Google Scholar]
  33. Pedersen F. S., Crowther R. L., Hays E. F., Nowinski R. C., Haseltine W. A. 1982; Structure of retroviral RNAs produced by cell lines derived from spontaneous lymphomas of AKR mice. Journal of Virology 41:18–29
    [Google Scholar]
  34. Rapp U. R. 1983; Kinetics of expression of infectious ecotropic, xenotropic and mink cell focus-forming murine leukemia virus after 5-iododeoxyuridine induction of cell from high- and low-leukemia mouse strains. Journal of Virology 45:755–765
    [Google Scholar]
  35. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  36. Rowe W. P., Hartley J. W., Lander M. R., Pugh W. E., Teich Y. 1971; Non-infectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology 46:866–876
    [Google Scholar]
  37. Scolnick E., Rands E., Aaronson S. A., Todaro G. J. 1970; RNA–dependent DNA polymerase activity in five RNA viruses: divalent cation requirements. Proceedings of the National Academy of Sciences, U.S.A 67:1789–1796
    [Google Scholar]
  38. Smith R., Morgan J. H. 1982; Identification of plaque isolates of an avian retrovirus causing rapid and slow onset of osteopetrosis. Virology 115:488–499
    [Google Scholar]
  39. Smith R. E., Moscovici C. 1969; The oncogenic effects of nontransforming viruses from avian myeloblastosis virus. Cancer Research 29:1356–1366
    [Google Scholar]
  40. Stone M. R., Nowinski R. C. 1980; Topological mapping of murine leukemia virus proteins by competitionbinding assay with monoclonal antibodies. Virology 100:370–381
    [Google Scholar]
  41. Van Gorp T. H. M., Swaen G. J. V. 1969; Myelofibrosis in rats experimentally infected with a murine leukemia virus. Journal of Pathology 97:235–240
    [Google Scholar]
  42. Vaughan J. M. 1973; The Effects of Irradiation on the Skeleton. Oxford: Clarendon Press;
    [Google Scholar]
  43. Waxdal M. J., Konigsberg W. H., Henley W. L., Edelmann G. M. 1968; The covalent structure of a human je G-immunoglobulin. Biochemistry 7:1959–1966
    [Google Scholar]
  44. Weber K. O., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate–polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-12-2237
Loading
/content/journal/jgv/10.1099/0022-1317-65-12-2237
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error