1887

Abstract

SUMMARY

In Berne virus-infected cells and in gradient-purified virions two major proteins with mol. wt. of 20K and 22K were detected. The 22K species is thought to represent the main envelope polypeptide; in infectious culture media it was present in a low density substructure which could be quantitatively converted into slowly sedimenting material by detergent treatment. The 20K polypeptide (accounting for about 80% of the C-amino acid label in the virion) was phosphorylated, occurred in an intracellular substructure of higher density than the virion (ρ = 1.36 g/ml in CsCl) and was the only viral protein possessing RNA-binding properties; it was recognized preferentially by heterologous animal sera in immune precipitation. The 20K species is therefore identified as the main capsid protein. Two additional polypeptides (19K and 17K) were regularly detected in extracts of infected cells; they appeared to share oligopeptides with the 20K protein and are interpreted as being proteolytic cleavage products. The nucleocapsid of Berne virus was visualized after ether treatment as a flexible bacilliform structure with conspicuous transverse striation. Demonstration of a 20K nucleocapsid protein further supports the authors′ proposal that Berne virus is a representative of a new family of enveloped RNA viruses (Toroviridae).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-6-1287
1985-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/6/JV0660061287.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-6-1287&mimeType=html&fmt=ahah

References

  1. Beards G. M., Hall C., Oreen J., Flewett T. H., Lamouliatte F., Du Pasquier P. 1984; An enveloped virus in stools of children and adults with gastroenteritis that resembles the Breda virus of calves. Lancet ii:1050–1052
    [Google Scholar]
  2. Bowen B., Steinberg J., Laemmli U. K., Weintraub H. 1980; The detection of DNA-binding proteins by protein blotting. Nucleic Acids Research 8:1–20
    [Google Scholar]
  3. Horzinek M. C. 1984; Nonarbo animal togaviruses and control perspectives. In Control of Virus Diseases pp 163–177 Edited by Kurstak E. (Papers from the 4th International Conference on Comparative Virology, Banff, Alberta, Canada, 1982.)
    [Google Scholar]
  4. Horzinek M. C., Weiss M. 1984; Toroviridae: a taxonomic proposal. Zentralblattfur Veterinarmedizin 31:649–659
    [Google Scholar]
  5. Horzinek M. C., Lutz H., Pedersen N. C. 1982; Antigenic relationships among homologous structural polypeptides of porcine, feline and canine coronaviruses. Infection and Immunity 37:1148–1155
    [Google Scholar]
  6. Horzinek M. C., Weiss M., Ederveen J. 1984; Berne virus is not ‘coronavirus-like’. Journal of General Virology 65:645–649
    [Google Scholar]
  7. Houmard J., Drapeau G. R. 1972; Staphylococcal protease: a proteolytic enzyme specific for glutamyl bonds. Proceedings of the National Academy of Sciences, U,. S,. A 69:3506–3509
    [Google Scholar]
  8. Laskey R. A., Mills A. D. 1975; Quantitative detection of3H andl4C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:331–335
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrok J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Fourth Report of the International Committee on Taxonomy of Viruses Basel; S. Karger.:
    [Google Scholar]
  11. Moussa A., Dannacher G., Fedida M. 1983; Nouveaux virus intervenant dans l’etiologie des ententes neonatales des bovins. Recueil de medecine veterinaire de L’Ecole d′AIfort 159:185–190
    [Google Scholar]
  12. Sokol F., Clark H. F. 1973; Phosphoproteins, structural components of rhabdoviruses. Virology 52:246–263
    [Google Scholar]
  13. Weiss M., Horzinek M. C. 1985; Resistance of Berne virus to physical and chemical treatment. Veterinary Microbiology (in press)
    [Google Scholar]
  14. Weiss M., Steck F., Horzinek M. C. 1983; Purification and partial characterization of a new enveloped RNA virus (Berne virus). Journal of General Virology 64:1849–1858
    [Google Scholar]
  15. Weiss M., Steck F., Kaderli R., Horzinek M. C. 1984; Antibodies to Berne virus in horses and other animals. Veterinary Microbiology 9:523–531
    [Google Scholar]
  16. Woode G. N., Reed D. E., Runnels P. L., Herrig M. A., Hill H. T. 1982; Studies with an unclassified virus isolated from diarrheic calves. Veterinary Microbiology 7:221–240
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-6-1287
Loading
/content/journal/jgv/10.1099/0022-1317-66-6-1287
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error