Virus-specific IgM and IgG Antibody Production by B Cells during Herpes Simplex Virus Type 2-induced Immunosuppression as Analysed by an Immunospot Assay
Nick, S. and Metzger, B. and Muller, S. and Falke, D.,, 68, 1951-1959 (1987),
doi = https://doi.org/10.1099/0022-1317-68-7-1951,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= SUMMARY The mechanism of herpes simplex virus (HSV)-2-induced immunosuppression was analysed by determination of the number of IgM and IgG antibody-secreting B cells in female BALB/c mice using an immunospot assay. Primary HSV-1 or -2 as well as homologous or heterologous booster infections at different times were performed. In accordance with earlier results on humoral antibody generation, in contrast to HSV-1, HSV-2 induced only very low numbers of antibody-producing B cells in dose-response experiments. They appeared late after infection compared to HSV-1. Despite a homologous humoral booster reaction against HSV-1 at day 8 no IgM- or IgG-secreting cells in the spleen could be detected. This non-reactivity of the spleen had vanished 10 days later, when secondary reactions of B cells could be observed. Secondary infections with a high homologous dose of HSV-2 after a low primary dose produced only a low booster response of IgG-secreting B cells. Suppression of humoral antibody production induced by HSV-2 (high dose) waned after more than 50 days, indicating that the HSV- 2-induced suppression did not impair antigen presentation or memory cell generation.,
language=,
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