1887

Abstract

Summary

Some properties of virus (TrV), a picorna-like virus recently isolated from , have been studied. Electron microscopic observations of purified viral preparations showed the presence of non-enveloped viral particles 30 nm in diameter. The sedimentation coefficient of virus particles was about 165S and the buoyant density in CsCl was 1.39 g/ml. The viral genome was composed of one single-stranded RNA molecule with an of 3 × 10. Three major polypeptides with values of 39K, 37K and 33K and a minor one of about 45K were found in the virus particle. TrV particles contain about 35% RNA and 65% protein by weight. These data support the classification of this virus in the family .

Keyword(s): picornavirus , Triatoma infestans and TrV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-11-2929
1988-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/11/JV0690112929.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-11-2929&mimeType=html&fmt=ahah

References

  1. BACHRACH H. L. 1968; Foot and mouth disease virus. Annual Review of Microbiology 2:201–244
    [Google Scholar]
  2. DAHLBERG A. E., DINGHAM C. W., PEACOCK A. C. 1969; Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. Journal of Molecular Biology 41:139–146
    [Google Scholar]
  3. DENOYA C. D., SCODELLER E. A., GIMENEZ B. H., VASQUEZ C., LA TORRE J. L. 1978; Foot and mouth disease virus. I. Stability of its ribonucleic acid. Virology 84:174–179
    [Google Scholar]
  4. GRACE T. C. C. 1962; Establishment of four strains of cells from insect tissues grown. in vitro. Nature, London 195:788–789
    [Google Scholar]
  5. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  7. MCMASTER G. K., CARMICHAEL G. C. 1977; Analysis of single- and double-stranded nucleic acids on Polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  8. MATHEKA H. D., BACHRACH H. L. 1975; N-terminal amino acid sequences in the major capsid proteins of foot and mouth disease virus types A, O and C. Journal of Virology 16:1248–1253
    [Google Scholar]
  9. MATTHEWS R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  10. MERRIL C. R., GOLDMAN D., SEDMAN S. A., EBERT M. H. 1981; Ultrasensitive stain for proteins in Polyacrylamide gels shows regional variations in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  11. MOORE N. F., KEARNS A., PULLIN J. S. K. 1980; Characterization of cricket paralysis virus-induced polypeptides in Drosophila cells. Virology 33:1–9
    [Google Scholar]
  12. MUSCIO O. A., LA TORRE J. L., SCODELLER E. A. 1987; Small nonoccluded viruses from triatomine bug Triatoma infestans (Hemiptera: Reduviidae). Journal of Invertebrate Pathology 49:218–220
    [Google Scholar]
  13. O’FARRELL P. H., GOODMAN H. M., O’FARRELL P. H. 1977; High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142
    [Google Scholar]
  14. PERRY R. P., LA TORRE J. L., KELLEY D. E., GREENBERG J. R. 1972; On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochimica et biophysica acta 262:220–226
    [Google Scholar]
  15. SHATKIN A. I. 1969 Colorimetric reactions for DNA, RNA and protein determination. Fundamental Techniques in Virology231–237 Edited by Hable K., Salzman N. P. New York & London: Academic Press;
    [Google Scholar]
  16. VASQUEZ C, DENOYA C. D., LA TORRE J. L., PALMA E. L. 1979; Structure of foot and mouth disease virus capsid. Virology 97:195–200
    [Google Scholar]
  17. VASQUEZ C, GRIGERA P. R., TISMINETZKY S. G., DENOYA C. D., LA TORRE J. L. 1981; Heterogeneity of infection-associated particles in foot and mouth disease virus. Virology 114:370–378
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-11-2929
Loading
/content/journal/jgv/10.1099/0022-1317-69-11-2929
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error