@article{mbs:/content/journal/jgv/10.1099/0022-1317-69-5-1079, author = "Stephanopoulos, Dimitrios E. and Kappes, John C. and Bernstein, David I.", title = "Enhanced in vitro Reactivation of Herpes Simplex Virus Type 2 from Latently Infected Guinea-pig Neural Tissues by 5-Azacytidine", journal= "Journal of General Virology", year = "1988", volume = "69", number = "5", pages = "1079-1083", doi = "https://doi.org/10.1099/0022-1317-69-5-1079", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-69-5-1079", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "latency", keywords = "methylation", keywords = "5-azacytidine", keywords = "HSV-2", abstract = "Summary 5-Azacytidine (5-AZC) reduces cytosine methylation in DNA and has been reported to activate quiescent virus genes. Treatment of explant cultures of latently herpes simplex virus type 2 (HSV-2)-infected guinea-pig dorsal root ganglia and spinal cords in vitro with 5-AZC significantly enhanced the rate of HSV recovery. Both the number of isolates from ganglia (P < 0.001) and the rate of recovery (P < 0.001) were significantly increased with the addition of 50 µM-5-AZC to explant cultures. Increased virus recovery appeared to be due to the induction of reactivation of latent virus, rather than an increase in replication, since 5-AZC inhibited HSV replication. These data support a role for methylation in HSV latency and reactivation.", }