@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-10-2811, author = "Staunton, David and Nuttall, Patricia A. and Bishop, David H. L.", title = "Sequence Analyses of Thogoto Viral RNA Segment 3: Evidence for a Distant Relationship between an Arbovirus and Members of the Orthomyxoviridae", journal= "Journal of General Virology", year = "1989", volume = "70", number = "10", pages = "2811-2817", doi = "https://doi.org/10.1099/0022-1317-70-10-2811", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-10-2811", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "arboviruses", keywords = "Thogoto virus", keywords = "orthomyxoviruses", abstract = "SUMMARY The genome of Thogoto (THO) virus, an unclassified tick-borne virus, comprises six segments of single-stranded RNA. The complete sequence of the third largest RNA segment has been determined from overlapping cDNA clones and by primer extension studies. Segment 3 RNA consists of 1865 nucleotides (approx. 6·2 × 105 M r). It has a large open reading frame (ORF1 ; 597 amino acids, 68·6K) in its virus-complementary sequence, confirming that the RNA has a negative-sense coding strategy. A transcription termination (polyadenylation) site located after the end of ORF1 has been identified. A second ORF (ORF2; 98 amino acids in length), overlapping ORF1, is also present in the virus-complementary sequence although whether it is translated is not known. The 3′ and 5′sequences of the segment 3 RNA are complementary and similar to those of the tick-borne Dhori (DHO) and the mammalian and avian influenza viruses. Protein database searches have identified regions of homology between the sequence of the THO ORF 1 gene product and regions of the PA protein of influenza virus strain A/NT/60/68 (approx. 20% aligned homology) and the corresponding protein of influenza B/Sing/222/79 virus (approx. 15% aligned homology). Although the THO protein sequence is not as closely related to those of the influenza viruses as they are to each other (40% aligned homology), the indicated sequence data provide further evidence of relationships between the tick-borne THO and DHO viruses and the vertebrate orthomyxoviruses.", }