Loss of Functional Voltage-gated Sodium Channels in Persistent Mumps Virus-infected PC12 Cells
Stauffer, Edward K. and Ziegler, Richard J.,, 70, 749-754 (1989),
doi = https://doi.org/10.1099/0022-1317-70-3-749,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= SUMMARY Rat pheochromocytoma (PC12) cells, persistently infected with mumps virus (MV), failed to generate full-sized stimulus-evoked action potentials (SEAPs) when examined by intracellular electrophysiological recording techniques. Application of tetrodotoxin (TTX) had little or no effect on MV-reduced SEAPs, indicating that the number of functional voltage-gated Na+ channels was decreased or their operation was blocked by the virus. In contrast, MV-infected cells generated normal Ca2+ spikes when bathed in a solution containing TTX, tetraethylammonium ions and a high concentration (20 mM) of Ca2+. In addition, when infected cells bathed in TTX were superfused with Co2+ the SEAP profile reverted to that typical of PC12 cells with functional voltage-gated K+ channels only. These observations indicate that MV affects voltage-gated Na+ channels, but spares voltage-gated Ca2+ and K+ channels of persistently infected cells.,
language=,
type=