1887

Abstract

SUMMARY

Recombinant baculoviruses expressing glycoprotein D (gD) of herpes simplex virus type 1 (HSV-1) have been generated. The proteins expressed from the recombinants have been characterized using monoclonal antibodies on Western blots and by immunoprecipitation. Partially glycosylated 48K polypeptides have been identified as products of the gD gene. Polyclonal sera from H-2k mice infected with HSV-1 recognized the same polypeptides. Furthermore, draining lymph node cells from H-2k mice infected with HSV-1 proliferated in response to recombinant-infected cell extracts. Immunization with such extracts generated high titre complement-dependent and -independent neutralizing antibody and the mice were protected against a challenge with HSV-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-7-1805
1989-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/7/JV0700071805.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-7-1805&mimeType=html&fmt=ahah

References

  1. Blacklaws B. A., Nash A. A., Darby G. 1987; Specificity of the immune response of mice to herpes simplex virus glycoproteins B and D constitutively expressed on L cell lines. Journal of General Virology 68:1103–1114
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Brown M., Faulkner P. 1977; A plaque assay for nuclear polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  4. Coelingh L. L. V. W., Murphy B. R., Collins P. L., Lebacq-Verheyden A. M., Battey J. F. 1987; Expression of biologically active and antigenically authentic parainfluenza type 3 hemagglutinin-neuraminidase glycoprotein by a recombinant baculovirus. Virology 160:465–472
    [Google Scholar]
  5. Cohen G. H., Isola V. J., Kuhns J., Berman P. W., Eisenberg R. I. 1986; Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing (‘native’) gel system of polyacrylamide gel electrophoresis coupled with Western blotting. Journal of Virology 60:157–166
    [Google Scholar]
  6. Chan W. L., Lukig M. L., Liew F. Y. 1985; Helper T cells induced by an immunopurified herpes simplex virus type 1 (HSV-1) 115 kilodalton glycoprotein B (gB) protect mice against HSV-1 infection. Journal of Experimental Medicine 162:1304–1318
    [Google Scholar]
  7. Cremer K. J., Macket M., Wohlenberg C., Notkins A., Moss B. 1985; Vaccinia virus recombinant expressing herpes simplex type 1 glycoprotein D prevents latent herpes in mice. Science 228:737–740
    [Google Scholar]
  8. Eisenberg R. J., Long D., Pereira L., Hampar B., Zweig B., Cohen G. H. 1982; Effect of monoclonal antibody on limited proteolysis of native glycoprotein D of herpes simplex type 1. Journal of Virology 41:478–488
    [Google Scholar]
  9. Eisenberg R. J., Long D., Ponce De Leon M., Mathews J. T., Spear P. G., Gibson M. G., Laskey L. A., Berman P., Golub E., Cohen G. H. 1985; Localization of epitopes of herpes simplex virus type 1 glycoprotein D. Journal of Virology 53:634–644
    [Google Scholar]
  10. Estes M. K., Crawford S. E., Penaranda M. E., Burns J., Chan W., Erickson B., Smithans G. E., Summers M. D. 1987; Synthesis and immunogenicity of rotavirus major capsid antigen using a baculovirus expression system. Journal of Virology 61:1488–1494
    [Google Scholar]
  11. Glorioso J., Kees U., Kumel G., Kircher H., Krammer P. H. 1985; Identification of herpes simplex virus type 1 (HSV-1) glycoprotein gC as the immunodominant antigen for HSV-1 specific memory cytotoxic T lymphocytes. Journal of Immunology 135:575–582
    [Google Scholar]
  12. Gompels U., Minson A. 1986; The properties and sequence of glycoprotein H of herpes simplex virus type 1. Virology 153:230–247
    [Google Scholar]
  13. Hu S. L., Kosowski S. G., Schaaf K. F. 1987; Expression of envelope glycoproteins of human immunodeficiency virus by an insect virus vector. Journal of Virology 61:3617–3620
    [Google Scholar]
  14. Kuroda K., Hauser C., Rott R., Klenk H., Doerfler W. 1986; Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO Journal 5:1359–1365
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227:680–685
    [Google Scholar]
  16. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Biotechnology 6:47–55
    [Google Scholar]
  17. Mclaughlin-Taylor E., Willey D. E., Cantin E. D., Eberle R., Moss B., Openshaw H. 1988; A recombinant vaccinia virus expressing herpes simplex virus type 1 glycoprotein B induces cytotoxic T lymphocytes in mice. Journal of General Virology 69:1731–1734
    [Google Scholar]
  18. Marsden H. S. 1987; Herpes simplex virus glycoproteins and pathogenesis. In Molecular Basis of Virus DiseaseSociety for General Microbiology Symposium40 Russell W. C., Almond J. W. Cambridge: Cambridge University Press;
    [Google Scholar]
  19. Martin S., Rouse B. T. 1987; The mechanism of antiviral immunity induced by a vaccinia recombinant expressing herpes simplex virus type 1 glycoprotein D. II. Clearance of local infection. Journal of Immunology 138:3431–3437
    [Google Scholar]
  20. Martin S., Moss B., Berman P. W., Laskey L. A., Rouse B. T. 1987; Mechanisms of antiviral immunity induced by a vaccinia recombinant expressing herpes simplex virus type 1 glycoprotein D. Journal of Virology 61:726–734
    [Google Scholar]
  21. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  22. Minson A. C., Hodgman T. C., Digard P., Hancock D. C., Bell S. E., Buckmaster E. A. 1986; An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. Journal of General Virology 67:1001–1013
    [Google Scholar]
  23. Nash A. A., Leung K. N., Wildy P. 1985; The T cell mediated immune response of mice to HSV. In The Herpesviruses 487–102 Roizman B., Lopez C. New York: Plenum Press;
    [Google Scholar]
  24. Norrild B. 1985; Humoral responses to herpes simplex infections. In The Herpesviruses 469–86 Roizman B., Lopez C. New York: Plenum Press;
    [Google Scholar]
  25. Overton H. A., Ihara T., Bishop D. H. L. 1987; Identification of the N and NSs proteins coded by the ambisense S RNA of Punta Toro phlebovirus using monospecific antisera raised to baculovirus expressed N and NSs proteins. Virology 157:338–350
    [Google Scholar]
  26. Possee R. D. 1986; Cell surface expression of influenza virus haemagglutinin in insect cells using a baculovirus vector. Virus Research 5:43–59
    [Google Scholar]
  27. Rosenthal K. L., Smiley J. R., South S., Johnson D. C. 1987; Cells expressing herpes simplex virus glycoprotein gC but not gB, gD, or gE are recognized by murine virus-specific cytotoxic T lymphocytes. Journal of Virology 61:2438–2447
    [Google Scholar]
  28. Simmons A., Nash A. A. 1985; Role of antibody in primary and recurrent herpes simplex infections. Journal of Virology 53:944–948
    [Google Scholar]
  29. Sullivan V., Smith G. L. 1987; Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by a recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody. Journal of General Virology 68:2587–2598
    [Google Scholar]
  30. Sullivan V., Smith G. L. 1988; The herpes simplex virus type 1 US7 gene product is a 66K glycoprotein and is a target for complement-dependent neutralization. Journal of General Virology 69:859–867
    [Google Scholar]
  31. Watson R. J., Weiss H., Salstrom J. S., Enquist L. W. 1982; HSV-1 glycoprotein D gene: nucleotide sequence and expression in E. coli. Science 218:381–384
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-7-1805
Loading
/content/journal/jgv/10.1099/0022-1317-70-7-1805
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error