1887

Abstract

An antiserum was raised against a fusion protein containing part of the 56K polypeptide (P5) encoded by the open reading frame (ORF) at the 3′ end of the genome of potato leafroll virus (PLRV). This antiserum reacted specifically with 80K and 90K polypeptides in PLRV-infected protoplasts, with a 90K polypeptide in infected potato tissue and with a 53K polypeptide in protein extracted from purified particles of PLRV. Monoclonal antibodies raised against purified PLRV particles also reacted with these polypeptides, as well as with the 23K coat protein. Virus particles partially purified from infected protoplasts contained some 90K polypeptide as well as the major 23K coat protein. The ORFs of the 23K coat protein and P5 are contiguous and in frame. The results suggest that the P5 polypeptide of PLRV occurs in infected cells as part of a readthrough protein comprising the 23K coat protein joined to the P5 amino acid sequence. Moreover the readthrough protein can be assembled into virus particles as a minor component together with the main 23K component. The P5 protein may thus contribute to properties of PLRV determined by its virus particle surface.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-10-2251
1990-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/10/JV0710102251.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-10-2251&mimeType=html&fmt=ahah

References

  1. Harrison B. D. 1984; Potato leafroll virus. CMI/AAB Descriptions of Plant Viruses291
    [Google Scholar]
  2. Hsu Y. H., Brakke M. K. 1985; Cell-free translation of soil-borne wheat mosaic virus RNAs. Virology 143:272–279
    [Google Scholar]
  3. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  5. Massalski P. R., Harrison B. D. 1987; Properties of monoclonal antibodies to potato leafroll luteovirus and their use to distinguish virus isolates differing in aphid transmissibility. Journal of General Virology 68:1813–1821
    [Google Scholar]
  6. Mayo M. A., Barker H. 1983; Effects of actinomycin D on the infection of tobacco protoplasts by four viruses. Journal of General Virology 64:1775–1780
    [Google Scholar]
  7. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70:1037–1051
    [Google Scholar]
  8. Miller W. A., Waterhouse P. M., Gerlach W. L. 1988; Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Research 16:6097–6111
    [Google Scholar]
  9. Morch M. D., Haenni A. L. 1987; Organization of plant virus genomes that comprise a single RNA molecule. In The Molecular Biology of the Positive Strand RNA Viruses pp 153–175 Rowlands D. J., Mayo M. A., Mahy B. W. J. Edited by London: Academic Press;
    [Google Scholar]
  10. Murant A. F., Waterhouse P. M., Raschké J. H., Robinson D. J. 1985; Carrot red leaf and carrot mottle viruses: observations on the composition of the particles in single and mixed infections. Journal of General Virology 66:1575–1579
    [Google Scholar]
  11. Nilsson B., Abrahamsen L., Uhlen M. 1985; Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO Journal 4:1075–1080
    [Google Scholar]
  12. Philipson L., Andersson P., Olshevsky U., Weinberg R., Baltimore D., Gesteland R. 1978; Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with a yeast suppressor tRNA. Cell 13:189–199
    [Google Scholar]
  13. Quillet L., Guilley H., Jonard G., Richards K. 1989; In vitrosynthesis of biologically active beet necrotic yellow vein virus RNA. Virology 172:293–301
    [Google Scholar]
  14. Randall R. E., Young D. F. 1988; Comparison between parainfluenza virus type 2 and simian virus 5: monoclonal antibodies reveal major antigenic differences. Journal of General Virology 69:2051–2060
    [Google Scholar]
  15. Rochow W. F., Duffus J. E. 1981; Luteoviruses and yellows diseases. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 147–170 Kurstak E. Edited by Amsterdam: Elsevier/ North-Holland;
    [Google Scholar]
  16. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukaemia virus. Nature; London: 293543–548
    [Google Scholar]
  17. Tamada T., Harrison B. D., Roberts I. M. 1984; Variation among British isolates of potato leafroll virus. Annals of Applied Biology 104:107–116
    [Google Scholar]
  18. Vanderwilk F., Huisman M. J., Cornelissen B. J. C., Huttinga H., Goldbach R. 1989; Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters 245:51–56
    [Google Scholar]
  19. Veidt I., Lot H., Leiser M., Sacheidecker D., Guilley H., Richards K. E., Jonard G. 1988; Nucleotide sequence of beet western yellows virus RNA. Nucleic Acids Research 16:9917–9932
    [Google Scholar]
  20. Waterhouse P. M. 1981 Purification, properties and relationships of carrot red leaf virus, and its interaction with carrot mottle virus Ph.D Thesis University of Dundee:
    [Google Scholar]
  21. Yoshinaka Y., Katoh I., Copeland T. D., Oroszlan S. 1985; Murine leukemia virus protease is encoded by the gag-polgene and is synthesized through suppression of an amber termination codon. Proceedings of the National Academy of Sciences U.S.A.: 821618–1622
    [Google Scholar]
  22. Ziegler V., Richards K., Guilley H., Jonard G., Putz C. 1985; Cell-free translation of beet necrotic yellow vein virus: readthrough of the coat protein cistron. Journal of General Virology 66:2079–2087
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-10-2251
Loading
/content/journal/jgv/10.1099/0022-1317-71-10-2251
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error