1887

Abstract

The nucleotide sequence of genome segment A cDNA of the STC strain of infectious bursal disease virus (IBDV) was determined and compared with sequences of the homologous genome segment of the 002–73 strain of IBDV and the Jasper strain of infectious pancreatic necrosis virus (IPNV). The STC-IBDV genome segment A was determined to be 3262 base pairs (bp), which is close to the estimated total length of 3300 bp for genome segment A in IBDV, although there is no proof that it is the real length of this genome segment. The STC-IBDV genome segment A contains two major overlapping open reading frames (ORFs). The large ORF of 3036 bp predicts a polyprotein of 109358, whereas the small ORF is 435 bp and predicts a protein of 16550 in STC-IBDV. STC-IBDV and 002–73-IBDV polyproteins are closely related (97·4% amino acid homology). Most of the amino acid mismatches are in VP2 sequences, mainly within the area of the conformation-dependent epitope. Comparison with the Jasper-IPNV polyprotein reveals levels of amino acid sequence homology of about 40% in VP2, 32% in VP3 and 21% in VP4. Within the VP2 molecule the conformation-dependent epitope area is again the least homologous, but the heterogeneity is more conspicuous than between the two IBDV strains, which is not surprising since IBDV and IPNV are serologically unrelated. The small ORF proteins have about 88% amino acid sequence homology between STC-IBDV and 002–73-IBDV, and 30% between each IBDV strain and Jasper-IPNV. There is no homology at all in the non-coding regions of IBDV and IPNV. These comparative sequence data will be useful for subgrouping the Birnaviridae family.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-569
1990-03-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/3/JV0710030569.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-3-569&mimeType=html&fmt=ahah

References

  1. Ansorge W., Labeit S. 1984; Field gradients improve resolution on DNA sequencing gels. Journal of Biochemical and Biophysical Methods 10:237–243
    [Google Scholar]
  2. Antczak J. B., Chmelo R., Pickup D. J., Joklik W. K. 1982; Sequences at both termini of the ten genes of reovirus serotype 3 (strain Dearing). Virology 121:307–319
    [Google Scholar]
  3. Azad A. A., Jagadish M. N., Brown M. A., Hudson P. J. 1987; Deletion mapping and expression in Escherichia coliof the large genomic segment of a bimavirus. Virology 161:145–152
    [Google Scholar]
  4. Becht H., Müller H., Müller H. K. 1988; Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. Journal of General Virology 69:631–640
    [Google Scholar]
  5. Birnboim H. C. 1983; A rapid alkaline extraction method for isolation of plasmid DNA. Methods in Enzymology 100:243–255
    [Google Scholar]
  6. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  7. Brown F. 1986; The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Sendai, September 1984. Intervirology 25:141–143
    [Google Scholar]
  8. Chen E. Y., Seeburg P. H. 1985; Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  9. Cheville N. F. 1967; Studies on the pathogenesis of Gumboro disease in the bursa of Fabricius, spleen and thymus of the chicken. American Journal of Pathology 51:527–551
    [Google Scholar]
  10. Clerx-Van Haaster C. M., Clerx J. P. M., Ushijima H., Akashi H., Fuller F., Bishop D. H. L. 1982; The 3ʹ terminal RNA sequences of bunyaviruses and nairoviruses (Bunyaviridae): evidence of end sequence generic differences within the virus family. Journal of General Virology 61:289–292
    [Google Scholar]
  11. Collins J. F., Coulson A. F. W. 1987; Molecular sequence comparison and alignment. In Nucleic Acid and Protein Sequence Analysis: A Practical Approach pp 323–358 Bishop M. J., Rawlings J. C. Edited by Washington: IRL Press;
    [Google Scholar]
  12. Dobos P., Hill B. J., Hallet R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. Journal of Virology 32:593–605
    [Google Scholar]
  13. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  14. Duncan R., Nagy E., Krell P. J., Dobos P. 1987; Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions. Journal of Virology 61:3655–3664
    [Google Scholar]
  15. Fahey K. J., Erny K., Crooks J. 1989; A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. Journal of General Virology 70:1473–1481
    [Google Scholar]
  16. Gouy M. 1987; Secondary structure prediction of RNA. In Nucleic Acid and Protein Sequence Analysis: A Practical Approach pp 259–284 Bishop M. J., Rawlings C. J. Edited by Oxford & Washington: IRL Press;
    [Google Scholar]
  17. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences U.S.A: 753961–3965
    [Google Scholar]
  18. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  19. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  20. Hudson P. J., McKern N. M., Power B. E., Azad A. A. 1986; Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Research 14:5001–5012
    [Google Scholar]
  21. Ismail N. M., Saif Y. M., Moorhead P. D. 1988; Lack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens. Avian Diseases 32:757–759
    [Google Scholar]
  22. Jackwood D. J., Kibenge F. S. B., Mercado C. C. 1989; The use of biotin labeled cDNA probes for the detection of infectious bursal disease viruses. Avian Diseases in press
    [Google Scholar]
  23. Jagadish M. N., Staton V. J., Hudson P. J., Azad A. A. 1988; Bimavirus precursor polyprotein is processed in Escherichia coliby its own virus-encoded polypeptide. Journal of Virology 62:1084–1087
    [Google Scholar]
  24. Kibenge F. S. B., Dhillon A. S., Russell R. G. 1988; Biochemistry and immunology of infectious bursal disease virus. Journal of General Virology 69:1757–1775
    [Google Scholar]
  25. Kimmel A. R., Berger S. L. 1987; Preparation of cDNA and the generation of cDNA libraries: overview. Methods in Enzymology 152:307–316
    [Google Scholar]
  26. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  27. Li J. K., Keene J. D., Scheible P. P., Joklik W. K. 1980; Nature of the 3ʹ terminal sequences of the plus and minus strands of the SI gene of reovirus serotypes 1, 2 and 3. Virology 105:41–51
    [Google Scholar]
  28. Macdonald R. D., Dobos P. 1981; Identification of the proteins encoded by each genome segment of infectious pancreatic necrosis virus. Virology 114:414–422
    [Google Scholar]
  29. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Morgan M. M., Macreadie I. G., Harley V. R., Hudson P. J., Azad A. A. 1988; Sequence of the small double-stranded RNA genomic segment of infectious bursal disease virus and its deduced 90-kDa product. Virology 163:240–242
    [Google Scholar]
  31. Muller H., Nitschke R. 1987a; Molecular weight determination of two segments of double-stranded RNA of infectious bursal disease virus, a member of the bimavirus group. Medical Microbiology and Immunology 176:113–121
    [Google Scholar]
  32. Muller H., Nitschke R. 1987b; The two segments of infectious bursal disease virus are circularized by a 90,000 Da protein. Virology 159:174–177
    [Google Scholar]
  33. Omura T., Minobe Y., Tsuchizaki T. 1988; Nucleotide sequence of segment S10 of the rice dwarf virus genome. Journal of General Virology 69:227–231
    [Google Scholar]
  34. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences U.S.A.: 852444–2448
    [Google Scholar]
  35. Porter A. G., Fellner P., Black D. N., Rowlands D. J., Harris T. J. R., Brown F. 1978; 3ʹ-Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature; London: 276298–301
    [Google Scholar]
  36. Raymond G. J., Bryant P. K.Jr Nelson A., Johnson J. D. 1988; Large-scale isolation of covalently closed circular DNA using gel filtration chromatography. Analytical Biochemistry 173:125–133
    [Google Scholar]
  37. Spies U., Muller H., Becht H. 1987; Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Research 8:127–140
    [Google Scholar]
  38. Winterfield R. W., Thacker H. L. 1978; Immune response and pathogenicity of different strains of infectious bursal disease virus applied as vaccines. Avian Diseases 22:721–731
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-569
Loading
/content/journal/jgv/10.1099/0022-1317-71-3-569
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error