1887

Abstract

Herpes simplex virus type 2 (HSV-2) causes lethal illness after intravaginal (IVAG) inoculation into BALB/cJ mice. In the present studies, we demonstrated in mice that primary IVAG vaccination with an attenuated strain of HSV-2 induced humoral immunity in sera and in vaginal secretions. Secondary genital exposure to HSV-2 enhanced this response. However, intraperitoneal exposure to attenuated HSV-2 elicited an antiviral antibody response in sera but not in vaginal secretions. In both sera and vaginal secretions, antiviral IgG antibodies were the major isotype. Systemic exposure to HSV-2 elicited antibodies only in sera that were specific for the major viral antigens whereas IVAG inoculation with HSV-2 stimulated both serum and vaginal antibody responses. Intravenous transfer of antiviral monoclonal antibodies protected against systemic HSV-2 infection but were ineffective against vaginal infection due to a lack of transudation into vaginal secretions. These results suggested that local humoral immunity in the genital tract is important in resistance to HSV-2.

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1990-07-01
2024-03-29
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References

  1. Ashley R., Mertz G., Clark H., Schick M., Saller D., Corey L. 1985; Humoral immune response to herpes simplex virus type 2 glycoproteins in patients receiving a glycoprotein subunit vaccine. Journal of Virology 56:475–481
    [Google Scholar]
  2. Balachandran N., Bacchetti S., Rawls W. E. 1982a; Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. Infection and Immunity 37:1132–1137
    [Google Scholar]
  3. Balachandran N., Harnish D., Rawls W. E., Bacchetti S. 1982b; Glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies. Journal of Virology 44:344–355
    [Google Scholar]
  4. Batty I., Warrack G. H. 1955; Local antibody production in the mammary gland, spleen, uterus, vagina and appendix of the rabbit. Journal of Pathology and Bacteriology 70:335–363
    [Google Scholar]
  5. Beer A. E., Heaves W. B. 1978; Antigenic status of semen from the viewpoints of the female and the male. Fertility and Sterility 29:3–22
    [Google Scholar]
  6. Berman P. W., Gregory T., Laskey L. A. 1985; Protection from genital herpes simplex type 2 infection by vaccination with cloned type 1 glycoprotein D. Science 227:1490–1492
    [Google Scholar]
  7. Bienenstock J., Befus A. D. 1980; Mucosal immunology. Immunology 41:249–270
    [Google Scholar]
  8. Blacklaws B. A., Nash A. A., Darby G. 1987; Specificity of the immune response of mice to herpes simplex virus glycoproteins B and D constitutively expressed on L cell lines. Journal of General Virology 68:1103–1114
    [Google Scholar]
  9. Brunham R. C., Kuo C.-C., Cles L., Holmes K. K. 1983; Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix. Infection and Immunity 39:1491–1494
    [Google Scholar]
  10. Chipperfield E. J., Evans B. A. 1972; The influence of local infection on immunoglobulin formation in the human endocervix. Clinical and Experimental Immunology 11:219–233
    [Google Scholar]
  11. Chipperfield E. J., Evans B. A. 1975; Effect of local infection and oral contraception on immunoglobulins in bovine cervicovaginal mucus; variation with parenteral immunization and local infection with Vibrio fetus . Journal of Reproduction and Fertility 31:359–365
    [Google Scholar]
  12. Clark D. A., Mcdermott M. R. 1981; Active suppression of host-vs-graft reactions in pregnant mice. Journal of Immunology 127:1267–1273
    [Google Scholar]
  13. Clark D. A., Slapsys R., Croy B. A., Rossant J., Mcdermott M. R. 1983; Regulation of cytotoxic T cells in pregnant mice. In Immunology of Reproduction pp. 343–361 Wegman T., Gill T. J. Edited by New York: Oxford University Press;
    [Google Scholar]
  14. Clarke D. N. 1984; Detection of antispermatozoal antibodies of IgG, IgA and IgM immunoglobulin classes in cervical mucus. American Journal of Reproductive Immunology 6:195–197
    [Google Scholar]
  15. Corey L., Spear P. G. 1986; Infections with herpes simplex viruses (parts 1 and 2). New England Journal of Medicine 314:686–691
    [Google Scholar]
  16. Cremer K. J., Mackett M., Wohlenberg C., Notkins A. B., Moss B. 1985; Vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D prevents latent herpes in mice. Science 228:737–740
    [Google Scholar]
  17. Engler H., Zawatzky R., Goldbach A., Schröder C. H., Weyand C., Hämmerling G. J., Kirchner H. 1981; Experimental infection of inbred mice with herpes simplex virus. II. Interferon production and activation of natural killer cells in the peritoneal exudate. Journal of General Virology 55:25–30
    [Google Scholar]
  18. Fitzgerald S.-A., Lopez C. 1982; Role of interferon in natural kill of herpes virus infected fibroblasts. In NK Cells and Other Natural Effector Cells pp. 287–393 Herberman R. B. Edited by New York: Academic Press;
    [Google Scholar]
  19. Johnson D. C., Feenstra V. 1987; Identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with gE and binds immunoglobulin. Journal of Virology 61:2208–2216
    [Google Scholar]
  20. Kerr W. R., Robertson M. 1953; Active and passive sensitization of the uterus of the cow in vivo against Trichomonas fetus antigen and evidence for the local production of antibody in that site. Journal of Hygiene 51:405–415
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  22. Larsen H. S., Russell R. G., Rouse B. T. 1983; Recovery from lethal herpes simplex virus type 1 infection is mediated by cytotoxic T lymphocytes. Infection and Immunity 41:197–204
    [Google Scholar]
  23. Leung K. N., Nash A. A., Sia D. Y., Wildy P. 1984; Clonal analysis of T-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper T-cell clone. Immunology 53:623–633
    [Google Scholar]
  24. Levitt D., Barol J. 1987; The immunobiology of Chlamydia . Immunology Today 8:246–251
    [Google Scholar]
  25. Ligas M., Johnson D. 1988; A herpes simplex virus mutant in which glycoprotein D sequences are replaced by β-galactosidase sequences binds to but is unable to penetrate into cells. Journal of Virology 62:1486–1494
    [Google Scholar]
  26. Long D., Madara T. J., Ponce de Leon M., Cohen G. H., Montgomery P. C., Eisenberg R. J. 1984; Glycoprotein D protects mice against lethal challenge with herpes simplex virus types 1 and 2. Infection and Immunity 37:761–764
    [Google Scholar]
  27. Mcdermott M. R., Bienenstock J. 1979; Evidence for a common mucosal immunologic system. I. Migration of B immunoblasts into intestinal, respiratory and genital tissues. Journal of Immunology 122:1892–1898
    [Google Scholar]
  28. Mcdermott M. R., Clark D. A., Bienenstock J. 1980; Evidence for a common mucosal immunologic system. II. Influence of the oestrous cycle on B immunoblast migration into genital and intestinal tissues. Journal of Immunology 124:2536–2539
    [Google Scholar]
  29. Mcdermott M. R., Befus A. D., Bienenstock J. 1982; The structural basis for immunity in the respiratory tract. International Review of Experimental Pathology 23:48–112
    [Google Scholar]
  30. Mcdermott M. R., Smiley J. R., Brais P. L. J., Rudzroga H. E., Bienenstock J. 1984; Immunity in the female genital tract after intravaginal vaccination of mice with an attenuated strain of herpes simplex virus type 2. Journal of Virology 51:747–753
    [Google Scholar]
  31. Mcdermott M. R., Brais P. L. J., Goettsche G. C., Evelegh M. J., Goldsmith C. H. 1987; Expression of immunity to intravaginal herpes simplex virus type 2 infection in the genital tract and associated lymph nodes. Archives of Virology 93:51–68
    [Google Scholar]
  32. Mcdermott M. R., Goldsmith C. H., Rosenthal K. L., Brais P. L. J. 1989; T lymphocytes in genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2. Journal of Infectious Diseases 159:460–466
    [Google Scholar]
  33. Mendis L. N., Best J. M., Banatvala J. G. 1981; Class-specific antibodies (IgG and IgA) to membrane antigens of herpes simplex type-2 infected cells in patients with cervical dysplasia and neoplasia. International Journal of Cancer 27:669–677
    [Google Scholar]
  34. Merrinran H., Woods S., Winter C., Fahnlander A., Corey L. 1984; Secretory IgA antibody in cervicovaginal secretions from women with genital infection due to herpes simplex virus. Journal of Infectious Diseases 149:505–510
    [Google Scholar]
  35. Morahan P. S., Breinig M. C., Mcgeorge M. B. 1977; Immune responses to vaginal or systemic infection of BALB/c mice with herpes simplex virus type 2. Journal of Immunology 119:2030–2036
    [Google Scholar]
  36. Morahan P. S., Morse S. S., Mcgeorge M. B. 1980; Macrophage extrinsic antiviral activity during herpes simplex virus infection. Journal of General Virology 46:291–300
    [Google Scholar]
  37. Nahmias A. J., Naib Z. M., Aighsmith A. K., Josey W. E. 1967; Experimental genital herpes simplex infection in the mouse. Pediatric Research 1:209–210
    [Google Scholar]
  38. Nash A. A., Ashford N. P. N. 1982; Split T cell tolerance in herpes simplex virus-infected mice and its implications for anti-viral immunity. Immunology 45:761–767
    [Google Scholar]
  39. Nash A. A., Gell P. G. H. 1983; Membrane phenotype of murine effector and suppressor T cells involved in delayed hypersensitivity and protective immunity to herpes simplex virus. Cellular Immunology 75:348–355
    [Google Scholar]
  40. Nash A. A., Gell P. G. H., Wildy P. 1981; Tolerance and immunity in mice infected with herpes simplex virus: simultaneous induction of protective immunity and tolerance to delayed-type hypersensitivity. Immunology 43:153–159
    [Google Scholar]
  41. Ninno G. R., Lew A. M., Stanley C. M., Steward M. W. 1984; Influence of antibody affinity on the performance of different antibody assays. Journal of Immunological Methods 72:177–187
    [Google Scholar]
  42. Ogra P. C., Ogra S. S. 1973; Local antibody response to polio vaccine in the human female genital tract. Journal of Immunology 110:1307–1311
    [Google Scholar]
  43. Parr M. B., Parr E. L. 1985; Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract. Journal of Reproduction and Fertility 74:361–370
    [Google Scholar]
  44. Plaeger-Marshall S., Wilson L. A., Smith J. W. 1983; Alteration of rabbit alveolar and peritoneal macrophage function by herpes simplex virus. Infection and Immunity 41:1376–1379
    [Google Scholar]
  45. Rawls W. E. 1985; Herpes simplex virus. In Virology pp. 527–561 Fields B. N. Edited by New York: Academic Press;
    [Google Scholar]
  46. Richmond S. J., Milne J. D., Hilton A. L., Caul E. O. 1980; Antibodies to Chlamydia trachomatis in cervicovaginal secretions: relation to serum antibodies and current chlamydial infections. Sexually Transmitted Diseases 7:11–15
    [Google Scholar]
  47. Rosenthal K. L., Smiley J. R., South S., Johnson D. C. 1987; Cells expressing herpes simplex virus glycoprotein gC but not gG, gD or gE are recognized by murine virus-specific cytotoxic T lymphocytes. Journal of Virology 61:2438–2447
    [Google Scholar]
  48. Sethi K. K., Omata Y., Schneweis K. E. 1983; Protection of mice from fatal herpes simplex virus type 1 infection by adoptive transfer of cloned virus specific and H-2-restricted cytotoxic T lymphocytes. Journal of General Virology 64:443–447
    [Google Scholar]
  49. Shore S. L., Cromeans T. L., Norrild B. 1979; Early damage of herpes-infected cells by antibody dependent cellular cytotoxicity: relative roles of virus-specified cell surface antigens and input virus. Journal of Immunology 123:2239–2244
    [Google Scholar]
  50. Simmons A., Nash A. A. 1985; Role of antibody in primary and recurrent herpes simplex virus infection. Journal of Virology 53:944–948
    [Google Scholar]
  51. Spear P. G. 1984; Glycoproteins specified by herpes simplex viruses. In The Herpes Viruses pp. 315–356 Roizman B. Edited by New York: Plenum Press;
    [Google Scholar]
  52. Tuffrey M., Falder P., Taylor-Robinson D. 1985; Effect on Chlamydia trachomatis infection of the murine genital tract of adoptive transfer of congenic immune cells or specific antibody. British Journal of Experimental Pathology 66:427–433
    [Google Scholar]
  53. Waldman R. H., Cruz J. M., Rowe D. S. 1972; Immunoglobulin levels and antibody to Candida albicans in human cervicovaginal secretions. Clinical and Experimental Immunology 10:427–434
    [Google Scholar]
  54. Walz M. A., Price R. W., Notkins A. L. 1974; Latent ganglionic infection with herpes simplex virus types 1 and 2: viral reactivation in vivo after neurectomy. Science 184:1175–1187
    [Google Scholar]
  55. Weisz-Carrington P., Roux M. E., Mcwilliams M., Phillips-Quagliata J. M., Lamm M. E. 1979; Organ and isotype distribution of plasma cells producing specific antibody after oral immunization: evidence for a generalized secretory immune system. Journal of Immunology 123:1705–1708
    [Google Scholar]
  56. Widders P. R., Stokes C. R., David J. S. E., Bourne F. J. 1985; Specific antibody in the equine genital tract following systemic and local immunization. Immunology 54:763–769
    [Google Scholar]
  57. Wilkie B. N., Duncan J. R., Winter A. J. 1972; The origin, class and specificity of immunoglobulins in bovine cervicovaginal mucus: variation with parenteral immunization and local infection with Vibrio fetus . Journal of Reproduction and Fertility 31:359–365
    [Google Scholar]
  58. Wira C. R., Sandoe C. P. 1977; Sex hormone regulation of IgA and IgG in rat uterine secretions. Nature; London: 268534–536
    [Google Scholar]
  59. Yang S.-L., Schumacher G. F. B. 1979; Immune response after vaginal application of antigens in the rhesus monkey. Fertility and Sterility 32:588–598
    [Google Scholar]
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