Characterization of the VPg-dsRNA linkage of infectious pancreatic necrosis virus
Calvert, Jay G. and Nagy, Eva and Soler, Maritza and Dobos, Peter,, 72, 2563-2567 (1991),
doi = https://doi.org/10.1099/0022-1317-72-10-2563,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of 125I-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VP1 (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5′ GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.,
language=,
type=