Bovine Immunodeficiency Virus: Immunochemical Characterization and Serological Survey
Horzinek, Marian and Keldermans, Lian and Stuurman, Thea and Black, John and Herrewegh, Arnold and Sillekens, Peter and Koolen, Marck,, 72, 2923-2928 (1991),
doi = https://doi.org/10.1099/0022-1317-72-12-2923,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with M r values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.,
language=,
type=