1887

Abstract

A recombinant baculovirus expressing the A antigen (A Ag) of Marek’s disease virus (MDV) was constructed. In (Sf) cells infected with the recombinant virus, A Ag expression was localized to the cell surface. Only a small amount of recombinant A Ag was detected in the culture supernatant of infected Sf cells, but authentic A Ag is mainly secreted into the culture supernatant of MDV-infected chicken embryo fibroblasts. Cell surface-associated recombinant A Ag seemed to be slightly larger than authentic A Ag, whereas the secreted recombinant A Ag seemed to be smaller. The recombinant A Ag was shown to be reactive with the sera of MDV-infected chickens by immunodiffusion studies and ELISA. Sera of chickens immunized with recombinant A Ag formed a precipitin line with the culture supernatant of MDV-infected chicken embryo fibroblasts in an immunodiffusion test. These results indicate that the recombinant A Ag expressed by the recombinant baculovirus retains the antigenic and immunogenic properties of the authentic A Ag.

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1991-05-01
2024-04-16
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References

  1. Binns M. M., Ross N. L. J. 1989; Nucleotide sequence of the Marek’s disease virus (MDV) RB-1B A antigengene and the identification of the MDV A antigen as the herpes simplex virus-1 glycoprotein C homologue. Virus Research 12:371–382
    [Google Scholar]
  2. Brown M., Faulkner P. 1977; A plaque assay for nuclear polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  3. Calnek B. W., Witter R. L. 1984; Marek’s disease. In Diseases of Poultry pp 325–360 Edited by Hofstad S. M., Barnes H. J., Reid W. M., Yoder W. H. Jr Ames: Iowa State University Press;
    [Google Scholar]
  4. Chubb R. C., Churchill A. E. 1968; Precipitating antibodies associated with Marek’s disease. Veterinary Record 83:4–7
    [Google Scholar]
  5. Churchill A. E., Chubb R. C., Baxendale W. 1969; The attenuation, with loss of oncogenicity, of the herpes-type viruses of Marek’s disease (strain hprs-16) on passage in cell culture. Journal of General Virology 4:557–564
    [Google Scholar]
  6. Coussens P. M., Velicer L. F. 1988; Structure and complete nucleotide sequence of the Marek’s disease herpesvirus gp57–65 gene. Journal of Virology 62:2373–2379
    [Google Scholar]
  7. Fukuchi K., Sudo M., Lee Y.-S., Tanaka A., Nonoyama M. 1984; Structure of Marek’s disease virus DNA: detailed restriction enzyme map. Journal of Virology 51:102–109
    [Google Scholar]
  8. Glaubiger C., Nazerian K., Velicer L. F. 1983; Marek’s disease herpesvirus. IV. Molecular characterization of Marek’s disease herpesvirus A antigen. Journal of Virology 45:1228–1234
    [Google Scholar]
  9. Ikuta K., Nishi Y., Kato S., Hirai K. 1981; Immunoprecipitation of Marekʼs disease virus-specific polypeptides with chicken antibodies purified by affinity chromatography. Virology 114:277–281
    [Google Scholar]
  10. Ikuta K., Ueda S., Kato S., Hirai K. 1983; Monoclonalantibodies reactive with the surface and secreted glycoproteins of Marek’s disease virus and herpesvirus of turkeys. Journal of General Virology 64:2597–2610
    [Google Scholar]
  11. Ikuta K., Ueda S., Kato S., Hirai K. 1984; Identification with monoclonal antibodies of glycoproteins of Marek’s disease virus and herpesvirus of turkeys related to virus neutralization. Journal of Virology 49:1014–1017
    [Google Scholar]
  12. Ikuta K., Nakajima K., Ueda S., Kato S., Hirai K. 1985; Differences in the processing of secreted glycoprotein A induced by Marek’s disease virus and herpesvirus of turkeys. Journal of General Virology 66:1131–1137
    [Google Scholar]
  13. Isfort R. J., Stringer R. A., Kung H.-J., Velicer L. F. 1986; Synthesis, processing, and secretion of the Marek’s disease herpesvirus A antigen glycoprotein. Journal of Virology 57:464–474
    [Google Scholar]
  14. Isfort R. J., Kung H.-J., Velicer L. F. 1987; Identification of the gene encoding Marek’s disease herpesvirus A antigen. Journal of Virology 61:2614–2620
    [Google Scholar]
  15. Jarvis D., Summers M. D. 1989; Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Molecular and Cellular Biology 9:214–223
    [Google Scholar]
  16. Kuroda K., Geyer H., Geyer R., Doerfler W., Klenk H. D. 1990; The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology 174:418–429
    [Google Scholar]
  17. Long P. A., Clark J. L., Velicer L. F. 1975a; Marekʼs disease herpesviruses. II. Purification and further characterization of Marek’s disease herpesvirus A antigen. Journal of Virology 15:1192–1201
    [Google Scholar]
  18. Long P. A., Kaveh-Yamini P., Velicer L. F. 1975b; Marek’s disease herpesviruses. I. Production and preliminary characterization of Marek’s disease herpesvirus A antigen. Journal of Virology 15:1182–1191
    [Google Scholar]
  19. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  20. Maeda S., Kawai T., Obinata M., Fujiwara H., Horiuchi T., Seki Y., Sato Y., Furusawa M. 1985; Production of human ##a- interferon in silkworm using a baculovirus vector. Nature, London 315:592–594
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982; Large scale isolation of plasmid DNA. In Molecular Cloning: A Laboratory Manual pp 86–96 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Matsuura Y., Possee R. D., Bishop D. H. L. 1986; Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector. Journal of General Virology 67:1515–1529
    [Google Scholar]
  23. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  24. Matsuura Y., Miyamoto M., Sato T., Morita C., Yasu K. 1989; Characterization of Japanese encephalitis virus envelope protein expressed by recombinant baculoviruses. Virology 173:674–682
    [Google Scholar]
  25. Nishikawa K., Isomura S., Suzuki S., Watanabe E., Hamaguchi M., Yoshida T., Nagai Y. 1983; Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparison. Virology 130:318–330
    [Google Scholar]
  26. Okazaki W., Purchase H. G., Burmester B. R. 1970; Protection against Marek’s disease by vaccination with herpesvirus of turkeys. Avian Disease 14:413–429
    [Google Scholar]
  27. Purchase H. G., Okazaki W., Burmester B. R. 1972; Long-term field trials with the herpesvirus of turkeys vaccine against Marek’s disease. Avian Disease 16:57–71
    [Google Scholar]
  28. Sharma J. M. 1980; Marek’s disease. In Isolation and Identification of Avian Pathogens pp 91–95 Edited by Hitchner B. S., Domermuth C. H., Purchase H. G., Williams J. E. Texas: American Association of Avian Pathologists;
    [Google Scholar]
  29. Smith G. E., Ju G., Ericson B. L., Moschera J., Lahm H.-W., Chizzonite R., Summers M. D. 1985; Modification and secretion of human interleukin 2 in insect cells by a baculovirus expression vector. Proceedings of the National Academy of Sciences, U.S.A. 82:8404–8408
    [Google Scholar]
  30. Van Zaane D., Brinkhof J. M. A., Westenbrink F., Gielkens A. L. J. 1982; Molecular-biological characterization of Marek’s disease virus. I. Identification of virus-specific polypeptides in infected cells. Virology 121:116–132
    [Google Scholar]
  31. Velicer L. F., Yager D. R., Clark J. L. 1978; Marek’s disease herpesviruses. III. Purification and characterization of Marek’s disease herpesvirus B antigen. Journal of Virology 27:205–217
    [Google Scholar]
  32. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
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