In vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis
Bossens, Michel and van Pachterbeke, Catherine and Tuynder, Marcel and Parent, Dominique and Heenen, Michel and Rommelaere, Jean,, 73, 3269-3273 (1992),
doi = https://doi.org/10.1099/0022-1317-73-12-3269,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (HPV-1), the agent responsible for deep plantar warts. Upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. Under these highly differentiating conditions, HPV-1 DNA amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 days thereafter. The extent of keratinocyte differentiation may be insufficient to allow a complete HPV infectious cycle.,
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